Accumulation of Antibiotic Resistance Genes in Carbapenem-Resistant <italic toggle="yes">Acinetobacter baumannii</italic> Isolates Belonging to Lineage 2, Global Clone 1, from Outbreaks in 2012–2013 at a Tehran Burns Hospital

ABSTRACT The worldwide distribution of carbapenem-resistant Acinetobacter baumannii (CRAB) has become a global concern, particularly in countries where antibiotic prescription is not tightly regulated. However, knowledge of the genomic aspects of CRAB from many parts of the world is still limited. H...

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Autores principales:	Masoumeh Douraghi, Johanna J. Kenyon, Parisa Aris, Mahla Asadian, Sedighe Ghourchian, Mohammad Hamidian
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	AbaR4 Acinetobacter baumannii GC1 Iran Tehran Tn2006 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/3139e310f44e441f96b30f38e56022b7
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spelling	oai:doaj.org-article:3139e310f44e441f96b30f38e56022b72021-11-15T15:29:17ZAccumulation of Antibiotic Resistance Genes in Carbapenem-Resistant <italic toggle="yes">Acinetobacter baumannii</italic> Isolates Belonging to Lineage 2, Global Clone 1, from Outbreaks in 2012–2013 at a Tehran Burns Hospital10.1128/mSphere.00164-202379-5042https://doaj.org/article/3139e310f44e441f96b30f38e56022b72020-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00164-20https://doaj.org/toc/2379-5042ABSTRACT The worldwide distribution of carbapenem-resistant Acinetobacter baumannii (CRAB) has become a global concern, particularly in countries where antibiotic prescription is not tightly regulated. However, knowledge of the genomic aspects of CRAB from many parts of the world is still limited. Here, 50 carbapenem-resistant A. baumannii isolates recovered at a single hospital in Tehran, Iran, during several outbreaks in 2012 and 2013 were found to be resistant to multiple antibiotics. They were examined using PCR mapping and multilocus sequence typing (MLST). All Iranian strains belonged to sequence type 328 in the Institut Pasteur MLST scheme (ST328IP), a single-locus variant of ST81IP, and all Iranian strains contained two carbapenem resistance genes, oxa23 and oxa24. The oxa23 gene is in the transposon Tn2006 in AbaR4, which interrupts the chromosomal comM gene. Phylogenetic analysis using whole-genome sequence (WGS) data for 9 isolates showed that they belonged to the same clade, designated the ST81/ST328 clade, within lineage 2 of global clone 1 (GC1). However, there were two groups that included either KL13 or KL18 at the K locus (KL) for capsular polysaccharide synthesis and either a tet39 or an aadB resistance gene, respectively. The genetic context of the resistance genes was determined, and the oxa24 (OXA-72 variant) and tet39 (tetracycline resistance) genes were each in a pdif module in different plasmids. The aadB gene cassette (which encodes gentamicin, kanamycin, and tobramycin resistance) was harbored by pRAY*, and the aphA6 gene (which encodes amikacin resistance) and sul2 gene (which encodes sulfamethoxazole resistance) were each harbored by a different plasmid. The sequences obtained here will underpin future studies of GC1 CRAB strains from the Middle East region. IMPORTANCE Carbapenem-resistant Acinetobacter baumannii strains are among the most critical antibiotic-resistant bacteria causing hospital-acquired infections and treatment failures. The global spread of two clones has been responsible for the bulk of the resistance, in particular, carbapenem resistance. However, there is a substantial gap in our knowledge of which clones and which specific lineages within each clone are circulating in many parts of the world, including Africa and the Middle East region. This is the first genomic analysis of carbapenem-resistant A. baumannii strains from Iran. All the isolates, from a single hospital, belonged to lineage 2 of global clone 1 (GC1) but fell into two groups distinguished by genes in the locus for capsule biosynthesis. The analysis suggests a potential origin of multiply antibiotic-resistant lineage 2 in the Middle East region and highlights the ongoing evolution of carbapenem-resistant GC1 A. baumannii strains. It will enhance future studies on the local and global GC1 population structure.Masoumeh DouraghiJohanna J. KenyonParisa ArisMahla AsadianSedighe GhourchianMohammad HamidianAmerican Society for MicrobiologyarticleAbaR4Acinetobacter baumanniiGC1IranTehranTn2006MicrobiologyQR1-502ENmSphere, Vol 5, Iss 2 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	AbaR4 Acinetobacter baumannii GC1 Iran Tehran Tn2006 Microbiology QR1-502
spellingShingle	AbaR4 Acinetobacter baumannii GC1 Iran Tehran Tn2006 Microbiology QR1-502 Masoumeh Douraghi Johanna J. Kenyon Parisa Aris Mahla Asadian Sedighe Ghourchian Mohammad Hamidian Accumulation of Antibiotic Resistance Genes in Carbapenem-Resistant <italic toggle="yes">Acinetobacter baumannii</italic> Isolates Belonging to Lineage 2, Global Clone 1, from Outbreaks in 2012–2013 at a Tehran Burns Hospital
description	ABSTRACT The worldwide distribution of carbapenem-resistant Acinetobacter baumannii (CRAB) has become a global concern, particularly in countries where antibiotic prescription is not tightly regulated. However, knowledge of the genomic aspects of CRAB from many parts of the world is still limited. Here, 50 carbapenem-resistant A. baumannii isolates recovered at a single hospital in Tehran, Iran, during several outbreaks in 2012 and 2013 were found to be resistant to multiple antibiotics. They were examined using PCR mapping and multilocus sequence typing (MLST). All Iranian strains belonged to sequence type 328 in the Institut Pasteur MLST scheme (ST328IP), a single-locus variant of ST81IP, and all Iranian strains contained two carbapenem resistance genes, oxa23 and oxa24. The oxa23 gene is in the transposon Tn2006 in AbaR4, which interrupts the chromosomal comM gene. Phylogenetic analysis using whole-genome sequence (WGS) data for 9 isolates showed that they belonged to the same clade, designated the ST81/ST328 clade, within lineage 2 of global clone 1 (GC1). However, there were two groups that included either KL13 or KL18 at the K locus (KL) for capsular polysaccharide synthesis and either a tet39 or an aadB resistance gene, respectively. The genetic context of the resistance genes was determined, and the oxa24 (OXA-72 variant) and tet39 (tetracycline resistance) genes were each in a pdif module in different plasmids. The aadB gene cassette (which encodes gentamicin, kanamycin, and tobramycin resistance) was harbored by pRAY*, and the aphA6 gene (which encodes amikacin resistance) and sul2 gene (which encodes sulfamethoxazole resistance) were each harbored by a different plasmid. The sequences obtained here will underpin future studies of GC1 CRAB strains from the Middle East region. IMPORTANCE Carbapenem-resistant Acinetobacter baumannii strains are among the most critical antibiotic-resistant bacteria causing hospital-acquired infections and treatment failures. The global spread of two clones has been responsible for the bulk of the resistance, in particular, carbapenem resistance. However, there is a substantial gap in our knowledge of which clones and which specific lineages within each clone are circulating in many parts of the world, including Africa and the Middle East region. This is the first genomic analysis of carbapenem-resistant A. baumannii strains from Iran. All the isolates, from a single hospital, belonged to lineage 2 of global clone 1 (GC1) but fell into two groups distinguished by genes in the locus for capsule biosynthesis. The analysis suggests a potential origin of multiply antibiotic-resistant lineage 2 in the Middle East region and highlights the ongoing evolution of carbapenem-resistant GC1 A. baumannii strains. It will enhance future studies on the local and global GC1 population structure.
format	article
author	Masoumeh Douraghi Johanna J. Kenyon Parisa Aris Mahla Asadian Sedighe Ghourchian Mohammad Hamidian
author_facet	Masoumeh Douraghi Johanna J. Kenyon Parisa Aris Mahla Asadian Sedighe Ghourchian Mohammad Hamidian
author_sort	Masoumeh Douraghi
title	Accumulation of Antibiotic Resistance Genes in Carbapenem-Resistant <italic toggle="yes">Acinetobacter baumannii</italic> Isolates Belonging to Lineage 2, Global Clone 1, from Outbreaks in 2012–2013 at a Tehran Burns Hospital
title_short	Accumulation of Antibiotic Resistance Genes in Carbapenem-Resistant <italic toggle="yes">Acinetobacter baumannii</italic> Isolates Belonging to Lineage 2, Global Clone 1, from Outbreaks in 2012–2013 at a Tehran Burns Hospital
title_full	Accumulation of Antibiotic Resistance Genes in Carbapenem-Resistant <italic toggle="yes">Acinetobacter baumannii</italic> Isolates Belonging to Lineage 2, Global Clone 1, from Outbreaks in 2012–2013 at a Tehran Burns Hospital
title_fullStr	Accumulation of Antibiotic Resistance Genes in Carbapenem-Resistant <italic toggle="yes">Acinetobacter baumannii</italic> Isolates Belonging to Lineage 2, Global Clone 1, from Outbreaks in 2012–2013 at a Tehran Burns Hospital
title_full_unstemmed	Accumulation of Antibiotic Resistance Genes in Carbapenem-Resistant <italic toggle="yes">Acinetobacter baumannii</italic> Isolates Belonging to Lineage 2, Global Clone 1, from Outbreaks in 2012–2013 at a Tehran Burns Hospital
title_sort	accumulation of antibiotic resistance genes in carbapenem-resistant <italic toggle="yes">acinetobacter baumannii</italic> isolates belonging to lineage 2, global clone 1, from outbreaks in 2012–2013 at a tehran burns hospital
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/3139e310f44e441f96b30f38e56022b7
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Accumulation of Antibiotic Resistance Genes in Carbapenem-Resistant <italic toggle="yes">Acinetobacter baumannii</italic> Isolates Belonging to Lineage 2, Global Clone 1, from Outbreaks in 2012–2013 at a Tehran Burns Hospital

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