Characterization of Five New Earthworm Mitogenomes (Oligochaeta: Lumbricidae): Mitochondrial Phylogeny of Lumbricidae
Identification based on conventional morphological characteristics is typically difficult and time-consuming. The development of molecular techniques provides a novel strategy that relies on specific mitochondrial gene fragments to conduct authentication. For this study, five newly sequenced partial...
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| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/313b4a3c381a403299eb76683320f4fd |
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| Sumario: | Identification based on conventional morphological characteristics is typically difficult and time-consuming. The development of molecular techniques provides a novel strategy that relies on specific mitochondrial gene fragments to conduct authentication. For this study, five newly sequenced partial mitogenomes of earthworms (<i>Bimastos parvus</i>, <i>Dendrobaena octaedra</i>, <i>Eisenia andrei</i>, <i>Eisenia nordenskioldi</i>, and <i>Octolasion tyrtaeum</i>) with lengths ranging from 14,977 to 15,715 were presented. Each mitogenome possessed a putative control region that resided between tRNA-Arg and tRNA-His. All of the PCGs were under negative selection according to the value of Ka/Ks. The phylogenetic trees supported the classification of <i>Eisenia</i> and <i>Lumbricus</i>; however, the trees based on <i>cox1</i> did not. Through various comparisons, it was determined that <i>cox1</i> fragments might be more suitable for molecular identification. These results lay the foundation for further phylogenetic studies on Lumbricidae. |
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