Antioxidant and cytoprotective effects of Nigella sativa L. seeds on the testis of monosodium glutamate challenged rats

Abstract Monosodium glutamate (MSG) is one of the most widely spread food additives that might cause male infertility. However, Nigella sativa L. seeds (NSS) could provide a solution. This study was designed to investigate the potential effects of NSS on rats ingesting MSG. To achieve this aim, adul...

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Autores principales:	Mahmoud Abd-Elkareem, Mokhless A. M. Abd El-Rahman, Nasser S. Abou Khalil, Ayman S. Amer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/313b4b67dc6740daad838969598e50c0
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spelling	oai:doaj.org-article:313b4b67dc6740daad838969598e50c02021-12-02T18:18:32ZAntioxidant and cytoprotective effects of Nigella sativa L. seeds on the testis of monosodium glutamate challenged rats10.1038/s41598-021-92977-42045-2322https://doaj.org/article/313b4b67dc6740daad838969598e50c02021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92977-4https://doaj.org/toc/2045-2322Abstract Monosodium glutamate (MSG) is one of the most widely spread food additives that might cause male infertility. However, Nigella sativa L. seeds (NSS) could provide a solution. This study was designed to investigate the potential effects of NSS on rats ingesting MSG. To achieve this aim, adult male albino rats were randomly equally assigned into three groups for 21 days: control group received no treatment, MSG group received MSG as 30 g/kg feed, and MSG + NSS group received MSG as 30 g/kg and NSS as 30 g/kg feed. Testis histomorphometry showed marked deterioration by MSG as atrophic seminiferous tubules with degeneration of their lining cells, damaged Leydig cells and decreased germ cells number. Periodic Acid Schiff stain indicated irregular interrupted basement membranes. Glutathione reductase, superoxide dismutase 2 (SOD2), and caspase-3 immuno-expressions increased in testicular cells. Testosterone levels were significantly decreased in MSG challenged rats along with significant increase in luteinizing hormone levels, whereas NSS normalized this hormonal profile. MSG exposure also caused significantly increased lipid peroxides (LPO), glutathione-S-transferase, and total antioxidant capacity (TAC) whereas nitric oxide and SOD2 were significantly decreased. NSS succeeded in rebalance LPO and TAC and ameliorated the histoarchitectural disturbances. NSS mitigated MSG-induced testicular impairment by its antioxidant and cytoprotective activities.Mahmoud Abd-ElkareemMokhless A. M. Abd El-RahmanNasser S. Abou KhalilAyman S. AmerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mahmoud Abd-Elkareem Mokhless A. M. Abd El-Rahman Nasser S. Abou Khalil Ayman S. Amer Antioxidant and cytoprotective effects of Nigella sativa L. seeds on the testis of monosodium glutamate challenged rats
description	Abstract Monosodium glutamate (MSG) is one of the most widely spread food additives that might cause male infertility. However, Nigella sativa L. seeds (NSS) could provide a solution. This study was designed to investigate the potential effects of NSS on rats ingesting MSG. To achieve this aim, adult male albino rats were randomly equally assigned into three groups for 21 days: control group received no treatment, MSG group received MSG as 30 g/kg feed, and MSG + NSS group received MSG as 30 g/kg and NSS as 30 g/kg feed. Testis histomorphometry showed marked deterioration by MSG as atrophic seminiferous tubules with degeneration of their lining cells, damaged Leydig cells and decreased germ cells number. Periodic Acid Schiff stain indicated irregular interrupted basement membranes. Glutathione reductase, superoxide dismutase 2 (SOD2), and caspase-3 immuno-expressions increased in testicular cells. Testosterone levels were significantly decreased in MSG challenged rats along with significant increase in luteinizing hormone levels, whereas NSS normalized this hormonal profile. MSG exposure also caused significantly increased lipid peroxides (LPO), glutathione-S-transferase, and total antioxidant capacity (TAC) whereas nitric oxide and SOD2 were significantly decreased. NSS succeeded in rebalance LPO and TAC and ameliorated the histoarchitectural disturbances. NSS mitigated MSG-induced testicular impairment by its antioxidant and cytoprotective activities.
format	article
author	Mahmoud Abd-Elkareem Mokhless A. M. Abd El-Rahman Nasser S. Abou Khalil Ayman S. Amer
author_facet	Mahmoud Abd-Elkareem Mokhless A. M. Abd El-Rahman Nasser S. Abou Khalil Ayman S. Amer
author_sort	Mahmoud Abd-Elkareem
title	Antioxidant and cytoprotective effects of Nigella sativa L. seeds on the testis of monosodium glutamate challenged rats
title_short	Antioxidant and cytoprotective effects of Nigella sativa L. seeds on the testis of monosodium glutamate challenged rats
title_full	Antioxidant and cytoprotective effects of Nigella sativa L. seeds on the testis of monosodium glutamate challenged rats
title_fullStr	Antioxidant and cytoprotective effects of Nigella sativa L. seeds on the testis of monosodium glutamate challenged rats
title_full_unstemmed	Antioxidant and cytoprotective effects of Nigella sativa L. seeds on the testis of monosodium glutamate challenged rats
title_sort	antioxidant and cytoprotective effects of nigella sativa l. seeds on the testis of monosodium glutamate challenged rats
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/313b4b67dc6740daad838969598e50c0
work_keys_str_mv	AT mahmoudabdelkareem antioxidantandcytoprotectiveeffectsofnigellasativalseedsonthetestisofmonosodiumglutamatechallengedrats AT mokhlessamabdelrahman antioxidantandcytoprotectiveeffectsofnigellasativalseedsonthetestisofmonosodiumglutamatechallengedrats AT nassersaboukhalil antioxidantandcytoprotectiveeffectsofnigellasativalseedsonthetestisofmonosodiumglutamatechallengedrats AT aymansamer antioxidantandcytoprotectiveeffectsofnigellasativalseedsonthetestisofmonosodiumglutamatechallengedrats
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Antioxidant and cytoprotective effects of Nigella sativa L. seeds on the testis of monosodium glutamate challenged rats

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