A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species
This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of <i>Melissa officinalis</i>, <i>Mentha</i> × <i>piperita</i>, <i>Ocimum basilicum</i>, <i>Rosma...
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2021
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oai:doaj.org-article:314079af2c6c47ff93a84651105ccd932021-11-25T18:45:06ZA Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species10.3390/plants101123062223-7747https://doaj.org/article/314079af2c6c47ff93a84651105ccd932021-10-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2306https://doaj.org/toc/2223-7747This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of <i>Melissa officinalis</i>, <i>Mentha</i> × <i>piperita</i>, <i>Ocimum basilicum</i>, <i>Rosmarinus officinalis</i>, <i>Salvia officinalis</i>, and <i>Satureja montana</i> (Lamiaceae), in different model systems. The polyphenols in these extracts were quantified both spectrophotometrically and using HPLC-DAD technique, while DPPH assay was used to assess the antioxidant activity. The genoprotective potential was tested on pUC19 <i>Escherichia coli</i> XL1-blue, and the antigenotoxicity on <i>Salmonella typhimurium</i> TA1535/pSK1002 and human lung fibroblasts, while the antitumor activity was assessed on colorectal cancer cells. Rosmarinic acid, quercetin, rutin, and luteolin-7-<i>O</i>-glucoside were among the identified compounds. Methanolic extracts had the best DPPH-scavenging and SOS-inducing activities, while ethanolic extracts exhibited the highest antigenotoxicity. Additionally, all extracts exhibited genoprotective potential on plasmid DNA. The antitumor effect was mediated by modulation of reactive oxygen species (ROS), nitric oxide (NO) production, and exhibition of genotoxic effects on tumor cells, especially with <i>O. basilicum</i> ethanolic extract. Generally, the investigated extracts were able to provide antioxidant protection for the acellular, prokaryotic, and normal human DNA, while also modulating the production of ROS and NO in tumor cells, leading to genotoxicity toward these cells and their decrease in proliferation.Mariana Oalđe PavlovićStoimir KolarevićJelena ĐorđevićJovana Jovanović MarićTanja LunićMarija MandićMargareta Kračun KolarevićJelena ŽivkovićAna Alimpić AradskiPetar D. MarinKatarina ŠavikinBranka Vuković-GačićBiljana Božić NedeljkovićSonja Duletić-LauševićMDPI AGarticleLamiaceaephenolic compoundsantioxidant activityantigenotoxicitygenoprotective effectantitumor activityBotanyQK1-989ENPlants, Vol 10, Iss 2306, p 2306 (2021) |
| institution |
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| collection |
DOAJ |
| language |
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| topic |
Lamiaceae phenolic compounds antioxidant activity antigenotoxicity genoprotective effect antitumor activity Botany QK1-989 |
| spellingShingle |
Lamiaceae phenolic compounds antioxidant activity antigenotoxicity genoprotective effect antitumor activity Botany QK1-989 Mariana Oalđe Pavlović Stoimir Kolarević Jelena Đorđević Jovana Jovanović Marić Tanja Lunić Marija Mandić Margareta Kračun Kolarević Jelena Živković Ana Alimpić Aradski Petar D. Marin Katarina Šavikin Branka Vuković-Gačić Biljana Božić Nedeljković Sonja Duletić-Laušević A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species |
| description |
This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of <i>Melissa officinalis</i>, <i>Mentha</i> × <i>piperita</i>, <i>Ocimum basilicum</i>, <i>Rosmarinus officinalis</i>, <i>Salvia officinalis</i>, and <i>Satureja montana</i> (Lamiaceae), in different model systems. The polyphenols in these extracts were quantified both spectrophotometrically and using HPLC-DAD technique, while DPPH assay was used to assess the antioxidant activity. The genoprotective potential was tested on pUC19 <i>Escherichia coli</i> XL1-blue, and the antigenotoxicity on <i>Salmonella typhimurium</i> TA1535/pSK1002 and human lung fibroblasts, while the antitumor activity was assessed on colorectal cancer cells. Rosmarinic acid, quercetin, rutin, and luteolin-7-<i>O</i>-glucoside were among the identified compounds. Methanolic extracts had the best DPPH-scavenging and SOS-inducing activities, while ethanolic extracts exhibited the highest antigenotoxicity. Additionally, all extracts exhibited genoprotective potential on plasmid DNA. The antitumor effect was mediated by modulation of reactive oxygen species (ROS), nitric oxide (NO) production, and exhibition of genotoxic effects on tumor cells, especially with <i>O. basilicum</i> ethanolic extract. Generally, the investigated extracts were able to provide antioxidant protection for the acellular, prokaryotic, and normal human DNA, while also modulating the production of ROS and NO in tumor cells, leading to genotoxicity toward these cells and their decrease in proliferation. |
| format |
article |
| author |
Mariana Oalđe Pavlović Stoimir Kolarević Jelena Đorđević Jovana Jovanović Marić Tanja Lunić Marija Mandić Margareta Kračun Kolarević Jelena Živković Ana Alimpić Aradski Petar D. Marin Katarina Šavikin Branka Vuković-Gačić Biljana Božić Nedeljković Sonja Duletić-Laušević |
| author_facet |
Mariana Oalđe Pavlović Stoimir Kolarević Jelena Đorđević Jovana Jovanović Marić Tanja Lunić Marija Mandić Margareta Kračun Kolarević Jelena Živković Ana Alimpić Aradski Petar D. Marin Katarina Šavikin Branka Vuković-Gačić Biljana Božić Nedeljković Sonja Duletić-Laušević |
| author_sort |
Mariana Oalđe Pavlović |
| title |
A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species |
| title_short |
A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species |
| title_full |
A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species |
| title_fullStr |
A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species |
| title_full_unstemmed |
A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species |
| title_sort |
study of phytochemistry, genoprotective activity, and antitumor effects of extracts of the selected lamiaceae species |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/314079af2c6c47ff93a84651105ccd93 |
| work_keys_str_mv |
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