Immunoinformatics mapping of potential epitopes in SARS-CoV-2 structural proteins.

All approved coronavirus disease 2019 (COVID-19) vaccines in current use are safe, effective, and reduce the risk of severe illness. Although data on the immunological presentation of patients with COVID-19 is limited, increasing experimental evidence supports the significant contribution of B and T...

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Autores principales:	Yengkhom Damayanti Devi, Himanshu Ballav Goswami, Sushmita Konwar, Chandrima Doley, Anutee Dolley, Arpita Devi, Chen Chongtham, Dikshita Dowerah, Vashkar Biswa, Latonglila Jamir, Aditya Kumar, Siddhartha Shankar Satapathy, Suvendra Kumar Ray, Ramesh Chandra Deka, Robin Doley, Manabendra Mandal, Sandeep Das, Chongtham Shyamsunder Singh, Partha Pratim Borah, Pabitra Nath, Nima D Namsa
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3141d809c7674c04ad839292d586c3f9
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spelling	oai:doaj.org-article:3141d809c7674c04ad839292d586c3f92021-12-02T20:16:21ZImmunoinformatics mapping of potential epitopes in SARS-CoV-2 structural proteins.1932-620310.1371/journal.pone.0258645https://doaj.org/article/3141d809c7674c04ad839292d586c3f92021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0258645https://doaj.org/toc/1932-6203All approved coronavirus disease 2019 (COVID-19) vaccines in current use are safe, effective, and reduce the risk of severe illness. Although data on the immunological presentation of patients with COVID-19 is limited, increasing experimental evidence supports the significant contribution of B and T cells towards the resolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Despite the availability of several COVID-19 vaccines with high efficacy, more effective vaccines are still needed to protect against the new variants of SARS-CoV-2. Employing a comprehensive immunoinformatic prediction algorithm and leveraging the genetic closeness with SARS-CoV, we have predicted potential immune epitopes in the structural proteins of SARS-CoV-2. The S and N proteins of SARS-CoV-2 and SARS-CoVs are main targets of antibody detection and have motivated us to design four multi-epitope vaccines which were based on our predicted B- and T-cell epitopes of SARS-CoV-2 structural proteins. The cardinal epitopes selected for the vaccine constructs are predicted to possess antigenic, non-allergenic, and cytokine-inducing properties. Additionally, some of the predicted epitopes have been experimentally validated in published papers. Furthermore, we used the C-ImmSim server to predict effective immune responses induced by the epitope-based vaccines. Taken together, the immune epitopes predicted in this study provide a platform for future experimental validations which may facilitate the development of effective vaccine candidates and epitope-based serological diagnostic assays.Yengkhom Damayanti DeviHimanshu Ballav GoswamiSushmita KonwarChandrima DoleyAnutee DolleyArpita DeviChen ChongthamDikshita DowerahVashkar BiswaLatonglila JamirAditya KumarSiddhartha Shankar SatapathySuvendra Kumar RayRamesh Chandra DekaRobin DoleyManabendra MandalSandeep DasChongtham Shyamsunder SinghPartha Pratim BorahPabitra NathNima D NamsaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0258645 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yengkhom Damayanti Devi Himanshu Ballav Goswami Sushmita Konwar Chandrima Doley Anutee Dolley Arpita Devi Chen Chongtham Dikshita Dowerah Vashkar Biswa Latonglila Jamir Aditya Kumar Siddhartha Shankar Satapathy Suvendra Kumar Ray Ramesh Chandra Deka Robin Doley Manabendra Mandal Sandeep Das Chongtham Shyamsunder Singh Partha Pratim Borah Pabitra Nath Nima D Namsa Immunoinformatics mapping of potential epitopes in SARS-CoV-2 structural proteins.
description	All approved coronavirus disease 2019 (COVID-19) vaccines in current use are safe, effective, and reduce the risk of severe illness. Although data on the immunological presentation of patients with COVID-19 is limited, increasing experimental evidence supports the significant contribution of B and T cells towards the resolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Despite the availability of several COVID-19 vaccines with high efficacy, more effective vaccines are still needed to protect against the new variants of SARS-CoV-2. Employing a comprehensive immunoinformatic prediction algorithm and leveraging the genetic closeness with SARS-CoV, we have predicted potential immune epitopes in the structural proteins of SARS-CoV-2. The S and N proteins of SARS-CoV-2 and SARS-CoVs are main targets of antibody detection and have motivated us to design four multi-epitope vaccines which were based on our predicted B- and T-cell epitopes of SARS-CoV-2 structural proteins. The cardinal epitopes selected for the vaccine constructs are predicted to possess antigenic, non-allergenic, and cytokine-inducing properties. Additionally, some of the predicted epitopes have been experimentally validated in published papers. Furthermore, we used the C-ImmSim server to predict effective immune responses induced by the epitope-based vaccines. Taken together, the immune epitopes predicted in this study provide a platform for future experimental validations which may facilitate the development of effective vaccine candidates and epitope-based serological diagnostic assays.
format	article
author	Yengkhom Damayanti Devi Himanshu Ballav Goswami Sushmita Konwar Chandrima Doley Anutee Dolley Arpita Devi Chen Chongtham Dikshita Dowerah Vashkar Biswa Latonglila Jamir Aditya Kumar Siddhartha Shankar Satapathy Suvendra Kumar Ray Ramesh Chandra Deka Robin Doley Manabendra Mandal Sandeep Das Chongtham Shyamsunder Singh Partha Pratim Borah Pabitra Nath Nima D Namsa
author_facet	Yengkhom Damayanti Devi Himanshu Ballav Goswami Sushmita Konwar Chandrima Doley Anutee Dolley Arpita Devi Chen Chongtham Dikshita Dowerah Vashkar Biswa Latonglila Jamir Aditya Kumar Siddhartha Shankar Satapathy Suvendra Kumar Ray Ramesh Chandra Deka Robin Doley Manabendra Mandal Sandeep Das Chongtham Shyamsunder Singh Partha Pratim Borah Pabitra Nath Nima D Namsa
author_sort	Yengkhom Damayanti Devi
title	Immunoinformatics mapping of potential epitopes in SARS-CoV-2 structural proteins.
title_short	Immunoinformatics mapping of potential epitopes in SARS-CoV-2 structural proteins.
title_full	Immunoinformatics mapping of potential epitopes in SARS-CoV-2 structural proteins.
title_fullStr	Immunoinformatics mapping of potential epitopes in SARS-CoV-2 structural proteins.
title_full_unstemmed	Immunoinformatics mapping of potential epitopes in SARS-CoV-2 structural proteins.
title_sort	immunoinformatics mapping of potential epitopes in sars-cov-2 structural proteins.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/3141d809c7674c04ad839292d586c3f9
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Immunoinformatics mapping of potential epitopes in SARS-CoV-2 structural proteins.

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