The CURE for Cultivating Fastidious Microbes

Course-Based Undergraduate Research Experiences (CUREs) expand the scientific educational benefits of research to large groups of students in a course setting. As part of an ongoing effort to integrate CUREs into first-year biology labs, we developed a microbiology CURE (mCURE) that uses a modified...

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Autores principales:	Arundhati Bakshi, Austen T. Webber, Lorelei E. Patrick, William Wischusen, Cameron Thrash
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/314273b6184745e685821e7e5d332031
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spelling	oai:doaj.org-article:314273b6184745e685821e7e5d3320312021-11-15T15:04:15ZThe CURE for Cultivating Fastidious Microbes10.1128/jmbe.v20i1.16351935-78851935-7877https://doaj.org/article/314273b6184745e685821e7e5d3320312019-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v20i1.1635https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885Course-Based Undergraduate Research Experiences (CUREs) expand the scientific educational benefits of research to large groups of students in a course setting. As part of an ongoing effort to integrate CUREs into first-year biology labs, we developed a microbiology CURE (mCURE) that uses a modified dilution-to-extinction high throughput culturing protocol for isolating abundant yet fastidious aquatic bacterioplankton during one semester. Students learn common molecular biology techniques like nucleic acid extraction, PCR, and molecular characterization; read and evaluate scientific literature; and receive training in scientific communication through written and oral exercises that incorporate social media elements. In the first three semesters, the mCUREs achieved similar cultivability success as implementation of the protocol in a standard laboratory setting. Our modular framework facilitates customization of the curriculum for use in multiple settings and we provide classroom exercises, assignments, assessment tools, and examples of student output to assist with implementation.Arundhati BakshiAusten T. WebberLorelei E. PatrickWilliam WischusenCameron ThrashAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 20, Iss 1 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Arundhati Bakshi Austen T. Webber Lorelei E. Patrick William Wischusen Cameron Thrash The CURE for Cultivating Fastidious Microbes
description	Course-Based Undergraduate Research Experiences (CUREs) expand the scientific educational benefits of research to large groups of students in a course setting. As part of an ongoing effort to integrate CUREs into first-year biology labs, we developed a microbiology CURE (mCURE) that uses a modified dilution-to-extinction high throughput culturing protocol for isolating abundant yet fastidious aquatic bacterioplankton during one semester. Students learn common molecular biology techniques like nucleic acid extraction, PCR, and molecular characterization; read and evaluate scientific literature; and receive training in scientific communication through written and oral exercises that incorporate social media elements. In the first three semesters, the mCUREs achieved similar cultivability success as implementation of the protocol in a standard laboratory setting. Our modular framework facilitates customization of the curriculum for use in multiple settings and we provide classroom exercises, assignments, assessment tools, and examples of student output to assist with implementation.
format	article
author	Arundhati Bakshi Austen T. Webber Lorelei E. Patrick William Wischusen Cameron Thrash
author_facet	Arundhati Bakshi Austen T. Webber Lorelei E. Patrick William Wischusen Cameron Thrash
author_sort	Arundhati Bakshi
title	The CURE for Cultivating Fastidious Microbes
title_short	The CURE for Cultivating Fastidious Microbes
title_full	The CURE for Cultivating Fastidious Microbes
title_fullStr	The CURE for Cultivating Fastidious Microbes
title_full_unstemmed	The CURE for Cultivating Fastidious Microbes
title_sort	cure for cultivating fastidious microbes
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/314273b6184745e685821e7e5d332031
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The CURE for Cultivating Fastidious Microbes

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