Protective Role of Combined Polyphenols and Micronutrients against Influenza A Virus and SARS-CoV-2 Infection In Vitro

Polyphenols have been widely studied for their antiviral effect against respiratory virus infections. Among these, resveratrol (RV) has been demonstrated to inhibit influenza virus replication and more recently, it has been tested together with pterostilbene against severe acute respiratory syndrome...

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Autores principales:	Marta De Angelis, David Della-Morte, Gabriele Buttinelli, Angela Di Martino, Francesca Pacifici, Paola Checconi, Luigina Ambrosio, Paola Stefanelli, Anna Teresa Palamara, Enrico Garaci, Camillo Ricordi, Lucia Nencioni
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	SARS-CoV-2 influenza virus antivirals polyphenols micronutrients COVID-19 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3144b63ceb8b4be4adc02d4e6d741ae1
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spelling	oai:doaj.org-article:3144b63ceb8b4be4adc02d4e6d741ae12021-11-25T16:51:19ZProtective Role of Combined Polyphenols and Micronutrients against Influenza A Virus and SARS-CoV-2 Infection In Vitro10.3390/biomedicines91117212227-9059https://doaj.org/article/3144b63ceb8b4be4adc02d4e6d741ae12021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1721https://doaj.org/toc/2227-9059Polyphenols have been widely studied for their antiviral effect against respiratory virus infections. Among these, resveratrol (RV) has been demonstrated to inhibit influenza virus replication and more recently, it has been tested together with pterostilbene against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. In the present work, we evaluated the antiviral activity of polydatin, an RV precursor, and a mixture of polyphenols and other micronutrients, named A5+, against influenza virus and SARS-CoV-2 infections. To this end, we infected Vero E6 cells and analyzed the replication of both respiratory viruses in terms of viral proteins synthesis and viral titration. We demonstrated that A5+ showed a higher efficacy in inhibiting both influenza virus and SARS-CoV-2 infections compared to polydatin treatment alone. Indeed, post infection treatment significantly decreased viral proteins expression and viral release, probably by interfering with any step of virus replicative cycle. Intriguingly, A5+ treatment strongly reduced IL-6 cytokine production in influenza virus-infected cells, suggesting its potential anti-inflammatory properties during the infection. Overall, these results demonstrate the synergic and innovative antiviral efficacy of A5+ mixture, although further studies are needed to clarify the mechanisms underlying its inhibitory effect.Marta De AngelisDavid Della-MorteGabriele ButtinelliAngela Di MartinoFrancesca PacificiPaola ChecconiLuigina AmbrosioPaola StefanelliAnna Teresa PalamaraEnrico GaraciCamillo RicordiLucia NencioniMDPI AGarticleSARS-CoV-2influenza virusantiviralspolyphenolsmicronutrientsCOVID-19Biology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1721, p 1721 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SARS-CoV-2 influenza virus antivirals polyphenols micronutrients COVID-19 Biology (General) QH301-705.5
spellingShingle	SARS-CoV-2 influenza virus antivirals polyphenols micronutrients COVID-19 Biology (General) QH301-705.5 Marta De Angelis David Della-Morte Gabriele Buttinelli Angela Di Martino Francesca Pacifici Paola Checconi Luigina Ambrosio Paola Stefanelli Anna Teresa Palamara Enrico Garaci Camillo Ricordi Lucia Nencioni Protective Role of Combined Polyphenols and Micronutrients against Influenza A Virus and SARS-CoV-2 Infection In Vitro
description	Polyphenols have been widely studied for their antiviral effect against respiratory virus infections. Among these, resveratrol (RV) has been demonstrated to inhibit influenza virus replication and more recently, it has been tested together with pterostilbene against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. In the present work, we evaluated the antiviral activity of polydatin, an RV precursor, and a mixture of polyphenols and other micronutrients, named A5+, against influenza virus and SARS-CoV-2 infections. To this end, we infected Vero E6 cells and analyzed the replication of both respiratory viruses in terms of viral proteins synthesis and viral titration. We demonstrated that A5+ showed a higher efficacy in inhibiting both influenza virus and SARS-CoV-2 infections compared to polydatin treatment alone. Indeed, post infection treatment significantly decreased viral proteins expression and viral release, probably by interfering with any step of virus replicative cycle. Intriguingly, A5+ treatment strongly reduced IL-6 cytokine production in influenza virus-infected cells, suggesting its potential anti-inflammatory properties during the infection. Overall, these results demonstrate the synergic and innovative antiviral efficacy of A5+ mixture, although further studies are needed to clarify the mechanisms underlying its inhibitory effect.
format	article
author	Marta De Angelis David Della-Morte Gabriele Buttinelli Angela Di Martino Francesca Pacifici Paola Checconi Luigina Ambrosio Paola Stefanelli Anna Teresa Palamara Enrico Garaci Camillo Ricordi Lucia Nencioni
author_facet	Marta De Angelis David Della-Morte Gabriele Buttinelli Angela Di Martino Francesca Pacifici Paola Checconi Luigina Ambrosio Paola Stefanelli Anna Teresa Palamara Enrico Garaci Camillo Ricordi Lucia Nencioni
author_sort	Marta De Angelis
title	Protective Role of Combined Polyphenols and Micronutrients against Influenza A Virus and SARS-CoV-2 Infection In Vitro
title_short	Protective Role of Combined Polyphenols and Micronutrients against Influenza A Virus and SARS-CoV-2 Infection In Vitro
title_full	Protective Role of Combined Polyphenols and Micronutrients against Influenza A Virus and SARS-CoV-2 Infection In Vitro
title_fullStr	Protective Role of Combined Polyphenols and Micronutrients against Influenza A Virus and SARS-CoV-2 Infection In Vitro
title_full_unstemmed	Protective Role of Combined Polyphenols and Micronutrients against Influenza A Virus and SARS-CoV-2 Infection In Vitro
title_sort	protective role of combined polyphenols and micronutrients against influenza a virus and sars-cov-2 infection in vitro
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3144b63ceb8b4be4adc02d4e6d741ae1
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Protective Role of Combined Polyphenols and Micronutrients against Influenza A Virus and SARS-CoV-2 Infection In Vitro

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