MiR-194 regulates chondrogenic differentiation of human adipose-derived stem cells by targeting Sox5.

Osteoarthritis, also known as degenerative arthritis or degenerative joint disease, causes pain and disability worldwide. Cartilage regeneration is key to finding a cure for this disease. Adipose-derived stem cells (ASCs) are capable of differentiating into cartilage lineages in vitro and they have...

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Autores principales:	Jun Xu, Yan Kang, Wei-ming Liao, Ling Yu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3145f140499348999fa83e4c9d5922e6
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spelling	oai:doaj.org-article:3145f140499348999fa83e4c9d5922e62021-11-18T07:26:19ZMiR-194 regulates chondrogenic differentiation of human adipose-derived stem cells by targeting Sox5.1932-620310.1371/journal.pone.0031861https://doaj.org/article/3145f140499348999fa83e4c9d5922e62012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22396742/?tool=EBIhttps://doaj.org/toc/1932-6203Osteoarthritis, also known as degenerative arthritis or degenerative joint disease, causes pain and disability worldwide. Cartilage regeneration is key to finding a cure for this disease. Adipose-derived stem cells (ASCs) are capable of differentiating into cartilage lineages in vitro and they have shown promise in the field of regenerative medicine. However, the underlying mechanisms remain unclear. In this study, we demonstrated that miR-194 levels gradually decreased during the chondrogenic differentiation of human ASCs (hASCs). After predicting the target of miR-194 using Pictar and Targetscan, we hypothesized that Sox5 is potentially the key link between miR-194 and the chondrogenesis of ASCs. Initially, we demonstrated that Sox5 is a target of miR194 according to luciferase assay analysis. We further demonstrated that the differentiation of ASCs can be controlled by miR-194 through gain or loss of function experiments, and we observed that the down-regulation of miR-194 increases its direct target gene, Sox5, and results in enhanced chondrogenic differentiation of hASCs, whereas up-regulation decreases Sox5 and inhibits chondrogenesis. We also found that miR-194 correlates with Sox5 in osteoarthritis. These findings, taken together, are the first to illustrate the critical role of miR-194 in hASC chondrogenesis, and may provide novel insight beneficial to cell manipulation methods during cartilage regeneration.Jun XuYan KangWei-ming LiaoLing YuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e31861 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jun Xu Yan Kang Wei-ming Liao Ling Yu MiR-194 regulates chondrogenic differentiation of human adipose-derived stem cells by targeting Sox5.
description	Osteoarthritis, also known as degenerative arthritis or degenerative joint disease, causes pain and disability worldwide. Cartilage regeneration is key to finding a cure for this disease. Adipose-derived stem cells (ASCs) are capable of differentiating into cartilage lineages in vitro and they have shown promise in the field of regenerative medicine. However, the underlying mechanisms remain unclear. In this study, we demonstrated that miR-194 levels gradually decreased during the chondrogenic differentiation of human ASCs (hASCs). After predicting the target of miR-194 using Pictar and Targetscan, we hypothesized that Sox5 is potentially the key link between miR-194 and the chondrogenesis of ASCs. Initially, we demonstrated that Sox5 is a target of miR194 according to luciferase assay analysis. We further demonstrated that the differentiation of ASCs can be controlled by miR-194 through gain or loss of function experiments, and we observed that the down-regulation of miR-194 increases its direct target gene, Sox5, and results in enhanced chondrogenic differentiation of hASCs, whereas up-regulation decreases Sox5 and inhibits chondrogenesis. We also found that miR-194 correlates with Sox5 in osteoarthritis. These findings, taken together, are the first to illustrate the critical role of miR-194 in hASC chondrogenesis, and may provide novel insight beneficial to cell manipulation methods during cartilage regeneration.
format	article
author	Jun Xu Yan Kang Wei-ming Liao Ling Yu
author_facet	Jun Xu Yan Kang Wei-ming Liao Ling Yu
author_sort	Jun Xu
title	MiR-194 regulates chondrogenic differentiation of human adipose-derived stem cells by targeting Sox5.
title_short	MiR-194 regulates chondrogenic differentiation of human adipose-derived stem cells by targeting Sox5.
title_full	MiR-194 regulates chondrogenic differentiation of human adipose-derived stem cells by targeting Sox5.
title_fullStr	MiR-194 regulates chondrogenic differentiation of human adipose-derived stem cells by targeting Sox5.
title_full_unstemmed	MiR-194 regulates chondrogenic differentiation of human adipose-derived stem cells by targeting Sox5.
title_sort	mir-194 regulates chondrogenic differentiation of human adipose-derived stem cells by targeting sox5.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/3145f140499348999fa83e4c9d5922e6
work_keys_str_mv	AT junxu mir194regulateschondrogenicdifferentiationofhumanadiposederivedstemcellsbytargetingsox5 AT yankang mir194regulateschondrogenicdifferentiationofhumanadiposederivedstemcellsbytargetingsox5 AT weimingliao mir194regulateschondrogenicdifferentiationofhumanadiposederivedstemcellsbytargetingsox5 AT lingyu mir194regulateschondrogenicdifferentiationofhumanadiposederivedstemcellsbytargetingsox5
_version_	1718423465677029376

MiR-194 regulates chondrogenic differentiation of human adipose-derived stem cells by targeting Sox5.

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