Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease

Abstract In this study, label-free fluorescence spectroscopy was used for the first time to determine spectral profiles of tryptophan, reduced nicotinamide adenine dinucleotide (NADH), and flavin denine dinucleotide (FAD) in fresh brain samples of a mouse model of Alzheimer’s disease (AD). Our resul...

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Autores principales: Lingyan Shi, Luyao Lu, George Harvey, Thomas Harvey, Adrián Rodríguez-Contreras, Robert R. Alfano
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Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/3147d78c140b4a9dbb2b094ac76b91a2
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spelling oai:doaj.org-article:3147d78c140b4a9dbb2b094ac76b91a22021-12-02T15:06:09ZLabel-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease10.1038/s41598-017-02673-52045-2322https://doaj.org/article/3147d78c140b4a9dbb2b094ac76b91a22017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02673-5https://doaj.org/toc/2045-2322Abstract In this study, label-free fluorescence spectroscopy was used for the first time to determine spectral profiles of tryptophan, reduced nicotinamide adenine dinucleotide (NADH), and flavin denine dinucleotide (FAD) in fresh brain samples of a mouse model of Alzheimer’s disease (AD). Our results showed that the emission spectral profile levels of tryptophan and NADH were higher in AD samples than normal samples. The intensity ratio of tryptophan to NADH and the change rate of fluorescence intensity with respect to wavelength also increased in AD brain. These results yield an optical method for detecting early stage of AD by comparing spectral profiles of biomolecules.Lingyan ShiLuyao LuGeorge HarveyThomas HarveyAdrián Rodríguez-ContrerasRobert R. AlfanoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Lingyan Shi
Luyao Lu
George Harvey
Thomas Harvey
Adrián Rodríguez-Contreras
Robert R. Alfano
Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease
description Abstract In this study, label-free fluorescence spectroscopy was used for the first time to determine spectral profiles of tryptophan, reduced nicotinamide adenine dinucleotide (NADH), and flavin denine dinucleotide (FAD) in fresh brain samples of a mouse model of Alzheimer’s disease (AD). Our results showed that the emission spectral profile levels of tryptophan and NADH were higher in AD samples than normal samples. The intensity ratio of tryptophan to NADH and the change rate of fluorescence intensity with respect to wavelength also increased in AD brain. These results yield an optical method for detecting early stage of AD by comparing spectral profiles of biomolecules.
format article
author Lingyan Shi
Luyao Lu
George Harvey
Thomas Harvey
Adrián Rodríguez-Contreras
Robert R. Alfano
author_facet Lingyan Shi
Luyao Lu
George Harvey
Thomas Harvey
Adrián Rodríguez-Contreras
Robert R. Alfano
author_sort Lingyan Shi
title Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease
title_short Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease
title_full Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease
title_fullStr Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease
title_full_unstemmed Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease
title_sort label-free fluorescence spectroscopy for detecting key biomolecules in brain tissue from a mouse model of alzheimer’s disease
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/3147d78c140b4a9dbb2b094ac76b91a2
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