Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease

Abstract In this study, label-free fluorescence spectroscopy was used for the first time to determine spectral profiles of tryptophan, reduced nicotinamide adenine dinucleotide (NADH), and flavin denine dinucleotide (FAD) in fresh brain samples of a mouse model of Alzheimer’s disease (AD). Our resul...

Full description

Saved in:

Bibliographic Details
Main Authors:	Lingyan Shi, Luyao Lu, George Harvey, Thomas Harvey, Adrián Rodríguez-Contreras, Robert R. Alfano
Format:	article
Language:	EN
Published:	Nature Portfolio 2017
Subjects:	Medicine R Science Q
Online Access:	https://doaj.org/article/3147d78c140b4a9dbb2b094ac76b91a2
Tags:	Add Tag No Tags, Be the first to tag this record!

id	oai:doaj.org-article:3147d78c140b4a9dbb2b094ac76b91a2
record_format	dspace
spelling	oai:doaj.org-article:3147d78c140b4a9dbb2b094ac76b91a22021-12-02T15:06:09ZLabel-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease10.1038/s41598-017-02673-52045-2322https://doaj.org/article/3147d78c140b4a9dbb2b094ac76b91a22017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02673-5https://doaj.org/toc/2045-2322Abstract In this study, label-free fluorescence spectroscopy was used for the first time to determine spectral profiles of tryptophan, reduced nicotinamide adenine dinucleotide (NADH), and flavin denine dinucleotide (FAD) in fresh brain samples of a mouse model of Alzheimer’s disease (AD). Our results showed that the emission spectral profile levels of tryptophan and NADH were higher in AD samples than normal samples. The intensity ratio of tryptophan to NADH and the change rate of fluorescence intensity with respect to wavelength also increased in AD brain. These results yield an optical method for detecting early stage of AD by comparing spectral profiles of biomolecules.Lingyan ShiLuyao LuGeorge HarveyThomas HarveyAdrián Rodríguez-ContrerasRobert R. AlfanoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lingyan Shi Luyao Lu George Harvey Thomas Harvey Adrián Rodríguez-Contreras Robert R. Alfano Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease
description	Abstract In this study, label-free fluorescence spectroscopy was used for the first time to determine spectral profiles of tryptophan, reduced nicotinamide adenine dinucleotide (NADH), and flavin denine dinucleotide (FAD) in fresh brain samples of a mouse model of Alzheimer’s disease (AD). Our results showed that the emission spectral profile levels of tryptophan and NADH were higher in AD samples than normal samples. The intensity ratio of tryptophan to NADH and the change rate of fluorescence intensity with respect to wavelength also increased in AD brain. These results yield an optical method for detecting early stage of AD by comparing spectral profiles of biomolecules.
format	article
author	Lingyan Shi Luyao Lu George Harvey Thomas Harvey Adrián Rodríguez-Contreras Robert R. Alfano
author_facet	Lingyan Shi Luyao Lu George Harvey Thomas Harvey Adrián Rodríguez-Contreras Robert R. Alfano
author_sort	Lingyan Shi
title	Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease
title_short	Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease
title_full	Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease
title_fullStr	Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease
title_full_unstemmed	Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease
title_sort	label-free fluorescence spectroscopy for detecting key biomolecules in brain tissue from a mouse model of alzheimer’s disease
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3147d78c140b4a9dbb2b094ac76b91a2
work_keys_str_mv	AT lingyanshi labelfreefluorescencespectroscopyfordetectingkeybiomoleculesinbraintissuefromamousemodelofalzheimersdisease AT luyaolu labelfreefluorescencespectroscopyfordetectingkeybiomoleculesinbraintissuefromamousemodelofalzheimersdisease AT georgeharvey labelfreefluorescencespectroscopyfordetectingkeybiomoleculesinbraintissuefromamousemodelofalzheimersdisease AT thomasharvey labelfreefluorescencespectroscopyfordetectingkeybiomoleculesinbraintissuefromamousemodelofalzheimersdisease AT adrianrodriguezcontreras labelfreefluorescencespectroscopyfordetectingkeybiomoleculesinbraintissuefromamousemodelofalzheimersdisease AT robertralfano labelfreefluorescencespectroscopyfordetectingkeybiomoleculesinbraintissuefromamousemodelofalzheimersdisease
_version_	1718388571845427200

Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease

Similar Items