Circulating miR-146a expression as a non-invasive predictive biomarker for acute lymphoblastic leukemia

Abstract Dysregulation of non-coding microRNAs during the course of tumor development, invasion and/or progression to the distant organs, makes them a promising candidate marker for the diagnosis of cancer and associated malignancies. This exploratory study aims at evaluating the usefulness of plasm...

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Autores principales:	Samiah Shahid, Wajeehah Shahid, Jawaria Shaheen, M. Waheed Akhtar, Saima Sadaf
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3151dd48426a45ab8b9d62c773b90e29
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spelling	oai:doaj.org-article:3151dd48426a45ab8b9d62c773b90e292021-11-28T12:17:20ZCirculating miR-146a expression as a non-invasive predictive biomarker for acute lymphoblastic leukemia10.1038/s41598-021-02257-42045-2322https://doaj.org/article/3151dd48426a45ab8b9d62c773b90e292021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02257-4https://doaj.org/toc/2045-2322Abstract Dysregulation of non-coding microRNAs during the course of tumor development, invasion and/or progression to the distant organs, makes them a promising candidate marker for the diagnosis of cancer and associated malignancies. This exploratory study aims at evaluating the usefulness of plasma concentration of circulating mir-146a as a non-invasive biomarker for acute lymphoblastic leukemia (ALL). Total RNA including miRNA was isolated from 110 plasma samples of patients (n = 66), healthy controls (n = 24) and follow up (n = 20) cases and reverse transcribed. Relative concentrations were assessed using real-time quantitative PCR and fold-change was calculated by 2−ΔΔCt method. Finally, relative concentrations were correlated to clinicopathological factors. Patients (n = 66) were analyzed to determine fold expression of miR-146a in plasma samples of ALL. Before chemotherapy, pediatric (n = 42) and adult (n = 24) showed overexpression of miR-146a compared with healthy controls (P < 0.0001). There was no effect of age and gender on mir-146a expression in plasma. mirR-146a expression was independent of clinical and hematological features. Moreover, miR-146a levels in plasma of paired samples (n = 20) after treatment showed significant decrease in expression (P < 0.001). Expression of plasma miR-146a may be utilized as non-invasive marker to diagnose and predict prognosis in pediatric and adult patients with ALL. Moreover predicted targets may be utilized for ALL therapy in future.Samiah ShahidWajeehah ShahidJawaria ShaheenM. Waheed AkhtarSaima SadafNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Samiah Shahid Wajeehah Shahid Jawaria Shaheen M. Waheed Akhtar Saima Sadaf Circulating miR-146a expression as a non-invasive predictive biomarker for acute lymphoblastic leukemia
description	Abstract Dysregulation of non-coding microRNAs during the course of tumor development, invasion and/or progression to the distant organs, makes them a promising candidate marker for the diagnosis of cancer and associated malignancies. This exploratory study aims at evaluating the usefulness of plasma concentration of circulating mir-146a as a non-invasive biomarker for acute lymphoblastic leukemia (ALL). Total RNA including miRNA was isolated from 110 plasma samples of patients (n = 66), healthy controls (n = 24) and follow up (n = 20) cases and reverse transcribed. Relative concentrations were assessed using real-time quantitative PCR and fold-change was calculated by 2−ΔΔCt method. Finally, relative concentrations were correlated to clinicopathological factors. Patients (n = 66) were analyzed to determine fold expression of miR-146a in plasma samples of ALL. Before chemotherapy, pediatric (n = 42) and adult (n = 24) showed overexpression of miR-146a compared with healthy controls (P < 0.0001). There was no effect of age and gender on mir-146a expression in plasma. mirR-146a expression was independent of clinical and hematological features. Moreover, miR-146a levels in plasma of paired samples (n = 20) after treatment showed significant decrease in expression (P < 0.001). Expression of plasma miR-146a may be utilized as non-invasive marker to diagnose and predict prognosis in pediatric and adult patients with ALL. Moreover predicted targets may be utilized for ALL therapy in future.
format	article
author	Samiah Shahid Wajeehah Shahid Jawaria Shaheen M. Waheed Akhtar Saima Sadaf
author_facet	Samiah Shahid Wajeehah Shahid Jawaria Shaheen M. Waheed Akhtar Saima Sadaf
author_sort	Samiah Shahid
title	Circulating miR-146a expression as a non-invasive predictive biomarker for acute lymphoblastic leukemia
title_short	Circulating miR-146a expression as a non-invasive predictive biomarker for acute lymphoblastic leukemia
title_full	Circulating miR-146a expression as a non-invasive predictive biomarker for acute lymphoblastic leukemia
title_fullStr	Circulating miR-146a expression as a non-invasive predictive biomarker for acute lymphoblastic leukemia
title_full_unstemmed	Circulating miR-146a expression as a non-invasive predictive biomarker for acute lymphoblastic leukemia
title_sort	circulating mir-146a expression as a non-invasive predictive biomarker for acute lymphoblastic leukemia
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3151dd48426a45ab8b9d62c773b90e29
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Circulating miR-146a expression as a non-invasive predictive biomarker for acute lymphoblastic leukemia

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