TRIM E3 ligases interfere with early and late stages of the retroviral life cycle.

Members of the TRIpartite interaction Motif (TRIM) family of E3 ligases have been shown to exhibit antiviral activities. Here we report a near comprehensive screen for antiretroviral activities of 55 TRIM proteins (36 human, 19 mouse). We identified approximately 20 TRIM proteins that, when transien...

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Autores principales:	Pradeep D Uchil, Brian D Quinlan, Wai-Tsing Chan, Joseph M Luna, Walther Mothes
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3152df98a5454c9d9bd6fbef1f78b15c
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spelling	oai:doaj.org-article:3152df98a5454c9d9bd6fbef1f78b15c2021-11-25T05:46:46ZTRIM E3 ligases interfere with early and late stages of the retroviral life cycle.1553-73661553-737410.1371/journal.ppat.0040016https://doaj.org/article/3152df98a5454c9d9bd6fbef1f78b15c2008-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18248090/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Members of the TRIpartite interaction Motif (TRIM) family of E3 ligases have been shown to exhibit antiviral activities. Here we report a near comprehensive screen for antiretroviral activities of 55 TRIM proteins (36 human, 19 mouse). We identified approximately 20 TRIM proteins that, when transiently expressed in HEK293 cells, affect the entry or release of human immunodeficiency virus 1 (HIV), murine leukemia virus (MLV), or avian leukosis virus (ALV). While TRIM11 and 31 inhibited HIV entry, TRIM11 enhanced N-MLV entry by interfering with Ref1 restriction. Strikingly, many TRIM proteins affected late stages of the viral life cycle. Gene silencing of endogenously expressed TRIM 25, 31, and 62 inhibited viral release indicating that they play an important role at late stages of the viral life cycle. In contrast, downregulation of TRIM11 and 15 enhanced virus release suggesting that these proteins contribute to the endogenous restriction of retroviruses in cells.Pradeep D UchilBrian D QuinlanWai-Tsing ChanJoseph M LunaWalther MothesPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 4, Iss 2, p e16 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Pradeep D Uchil Brian D Quinlan Wai-Tsing Chan Joseph M Luna Walther Mothes TRIM E3 ligases interfere with early and late stages of the retroviral life cycle.
description	Members of the TRIpartite interaction Motif (TRIM) family of E3 ligases have been shown to exhibit antiviral activities. Here we report a near comprehensive screen for antiretroviral activities of 55 TRIM proteins (36 human, 19 mouse). We identified approximately 20 TRIM proteins that, when transiently expressed in HEK293 cells, affect the entry or release of human immunodeficiency virus 1 (HIV), murine leukemia virus (MLV), or avian leukosis virus (ALV). While TRIM11 and 31 inhibited HIV entry, TRIM11 enhanced N-MLV entry by interfering with Ref1 restriction. Strikingly, many TRIM proteins affected late stages of the viral life cycle. Gene silencing of endogenously expressed TRIM 25, 31, and 62 inhibited viral release indicating that they play an important role at late stages of the viral life cycle. In contrast, downregulation of TRIM11 and 15 enhanced virus release suggesting that these proteins contribute to the endogenous restriction of retroviruses in cells.
format	article
author	Pradeep D Uchil Brian D Quinlan Wai-Tsing Chan Joseph M Luna Walther Mothes
author_facet	Pradeep D Uchil Brian D Quinlan Wai-Tsing Chan Joseph M Luna Walther Mothes
author_sort	Pradeep D Uchil
title	TRIM E3 ligases interfere with early and late stages of the retroviral life cycle.
title_short	TRIM E3 ligases interfere with early and late stages of the retroviral life cycle.
title_full	TRIM E3 ligases interfere with early and late stages of the retroviral life cycle.
title_fullStr	TRIM E3 ligases interfere with early and late stages of the retroviral life cycle.
title_full_unstemmed	TRIM E3 ligases interfere with early and late stages of the retroviral life cycle.
title_sort	trim e3 ligases interfere with early and late stages of the retroviral life cycle.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/3152df98a5454c9d9bd6fbef1f78b15c
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_version_	1718414450751438848

TRIM E3 ligases interfere with early and late stages of the retroviral life cycle.

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