Inactivation of Non-Enveloped Viruses and Bacteria by an Electrically Charged Disinfectant Containing Meso-Structure Nanoparticles via Modification of the Genome

Akikazu Sakudo,1,2 Risa Yamashiro,2 Makoto Haritani,3 Koichi Furusaki,4 Rumiko Onishi,5 Takashi Onodera3 1Faculty of Veterinary Medicine, Okayama University of Science, Imabari, Ehime 794-8555, Japan; 2Laboratory of Biometabolic Chemistry, School of Health Sciences, University of the Ryukyus, Nishih...

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Autores principales:	Sakudo A, Yamashiro R, Haritani M, Furusaki K, Onishi R, Onodera T
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	class i disinfectant e. coli feline calicivirus food safety meso-structure salmonella Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/31545ce7ffaf4c0591ed0388033f53fa
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spelling	oai:doaj.org-article:31545ce7ffaf4c0591ed0388033f53fa2021-12-02T03:22:47ZInactivation of Non-Enveloped Viruses and Bacteria by an Electrically Charged Disinfectant Containing Meso-Structure Nanoparticles via Modification of the Genome1178-2013https://doaj.org/article/31545ce7ffaf4c0591ed0388033f53fa2020-02-01T00:00:00Zhttps://www.dovepress.com/inactivation-of-non-enveloped-viruses-and-bacteria-by-an-electrically--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Akikazu Sakudo,1,2 Risa Yamashiro,2 Makoto Haritani,3 Koichi Furusaki,4 Rumiko Onishi,5 Takashi Onodera3 1Faculty of Veterinary Medicine, Okayama University of Science, Imabari, Ehime 794-8555, Japan; 2Laboratory of Biometabolic Chemistry, School of Health Sciences, University of the Ryukyus, Nishihara, Okinawa 903-0215, Japan; 3Research Center for Food Safety, The University of Tokyo, Bunkyo-Ku, Tokyo 113-8657, Japan; 4Mineral Activation Technical Research Center, Omuta, Fukuoka 836-0041, Japan; 5Santa Mineral Co., Ltd., Minato-Ku, Tokyo 105-0013, JapanCorrespondence: Akikazu SakudoFaculty of Veterinary Medicine, Okayama University of Science, Imabari, Ehime 794-8555, JapanTel/Fax +81-898-52-9198Email akikazusakudo@gmail.comIntroduction: A previous study demonstrated the virucidal effect of an electrically charged disinfectant (CAC-717), which contains meso-structure nanoparticles, on enveloped viruses (influenza viruses). However, the effect of CAC-717 on other microorganisms and the mechanisms by which CAC-717 inactivates the microorganisms remain unclear. In this study, CAC-717 was further evaluated in terms of its biocidal and virucidal activity as well as its effect on bacterial and viral nucleic acids.Methods: The inactivation effects of CAC-717 against various microorganisms [non-enveloped virus, feline calicivirus (FCV); bacteria, Salmonella enterica and Escherichia coli] were investigated by comparing the viral titer of the medium tissue culture infectious dose (TCID50) and the D value (estimated treatment time required to reduce the number of microorganisms by 90%). Furthermore, the effects of CAC-717 on viral and bacterial genomic RNA/DNA were examined using a polymerase chain reaction (PCR).Results: Treatment of an equal volume of CAC-717 with cell lysate infected with a non-enveloped virus, feline calicivirus (FCV), reduced the TCID50. Viral titer dropped below the detection limit after 2 min of treatment. The D value of FCV was 0.256 min (average of multiple endpoint D values) and endpoint D value was 0.341 min. The D value for E. coli and S. enterica was 0.290 min and 0.080 min (average of multiple endpoint D values), respectively and the endpoint D value was 0.545 min and 0.054 min, respectively. In addition, PCR showed the inhibition of nucleic acid amplification of the RNA and DNA genome of FCV and bacteria, respectively.Conclusion: Our findings suggest that CAC-717 inactivates viruses and bacteria by modifying the viral and bacterial nucleic acids.Keywords: class I disinfectant, E. coli, feline calicivirus, food safety, meso-structure, SalmonellaSakudo AYamashiro RHaritani MFurusaki KOnishi ROnodera TDove Medical Pressarticleclass i disinfectante. colifeline calicivirusfood safetymeso-structuresalmonellaMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 1387-1395 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	class i disinfectant e. coli feline calicivirus food safety meso-structure salmonella Medicine (General) R5-920
spellingShingle	class i disinfectant e. coli feline calicivirus food safety meso-structure salmonella Medicine (General) R5-920 Sakudo A Yamashiro R Haritani M Furusaki K Onishi R Onodera T Inactivation of Non-Enveloped Viruses and Bacteria by an Electrically Charged Disinfectant Containing Meso-Structure Nanoparticles via Modification of the Genome
description	Akikazu Sakudo,1,2 Risa Yamashiro,2 Makoto Haritani,3 Koichi Furusaki,4 Rumiko Onishi,5 Takashi Onodera3 1Faculty of Veterinary Medicine, Okayama University of Science, Imabari, Ehime 794-8555, Japan; 2Laboratory of Biometabolic Chemistry, School of Health Sciences, University of the Ryukyus, Nishihara, Okinawa 903-0215, Japan; 3Research Center for Food Safety, The University of Tokyo, Bunkyo-Ku, Tokyo 113-8657, Japan; 4Mineral Activation Technical Research Center, Omuta, Fukuoka 836-0041, Japan; 5Santa Mineral Co., Ltd., Minato-Ku, Tokyo 105-0013, JapanCorrespondence: Akikazu SakudoFaculty of Veterinary Medicine, Okayama University of Science, Imabari, Ehime 794-8555, JapanTel/Fax +81-898-52-9198Email akikazusakudo@gmail.comIntroduction: A previous study demonstrated the virucidal effect of an electrically charged disinfectant (CAC-717), which contains meso-structure nanoparticles, on enveloped viruses (influenza viruses). However, the effect of CAC-717 on other microorganisms and the mechanisms by which CAC-717 inactivates the microorganisms remain unclear. In this study, CAC-717 was further evaluated in terms of its biocidal and virucidal activity as well as its effect on bacterial and viral nucleic acids.Methods: The inactivation effects of CAC-717 against various microorganisms [non-enveloped virus, feline calicivirus (FCV); bacteria, Salmonella enterica and Escherichia coli] were investigated by comparing the viral titer of the medium tissue culture infectious dose (TCID50) and the D value (estimated treatment time required to reduce the number of microorganisms by 90%). Furthermore, the effects of CAC-717 on viral and bacterial genomic RNA/DNA were examined using a polymerase chain reaction (PCR).Results: Treatment of an equal volume of CAC-717 with cell lysate infected with a non-enveloped virus, feline calicivirus (FCV), reduced the TCID50. Viral titer dropped below the detection limit after 2 min of treatment. The D value of FCV was 0.256 min (average of multiple endpoint D values) and endpoint D value was 0.341 min. The D value for E. coli and S. enterica was 0.290 min and 0.080 min (average of multiple endpoint D values), respectively and the endpoint D value was 0.545 min and 0.054 min, respectively. In addition, PCR showed the inhibition of nucleic acid amplification of the RNA and DNA genome of FCV and bacteria, respectively.Conclusion: Our findings suggest that CAC-717 inactivates viruses and bacteria by modifying the viral and bacterial nucleic acids.Keywords: class I disinfectant, E. coli, feline calicivirus, food safety, meso-structure, Salmonella
format	article
author	Sakudo A Yamashiro R Haritani M Furusaki K Onishi R Onodera T
author_facet	Sakudo A Yamashiro R Haritani M Furusaki K Onishi R Onodera T
author_sort	Sakudo A
title	Inactivation of Non-Enveloped Viruses and Bacteria by an Electrically Charged Disinfectant Containing Meso-Structure Nanoparticles via Modification of the Genome
title_short	Inactivation of Non-Enveloped Viruses and Bacteria by an Electrically Charged Disinfectant Containing Meso-Structure Nanoparticles via Modification of the Genome
title_full	Inactivation of Non-Enveloped Viruses and Bacteria by an Electrically Charged Disinfectant Containing Meso-Structure Nanoparticles via Modification of the Genome
title_fullStr	Inactivation of Non-Enveloped Viruses and Bacteria by an Electrically Charged Disinfectant Containing Meso-Structure Nanoparticles via Modification of the Genome
title_full_unstemmed	Inactivation of Non-Enveloped Viruses and Bacteria by an Electrically Charged Disinfectant Containing Meso-Structure Nanoparticles via Modification of the Genome
title_sort	inactivation of non-enveloped viruses and bacteria by an electrically charged disinfectant containing meso-structure nanoparticles via modification of the genome
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/31545ce7ffaf4c0591ed0388033f53fa
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Inactivation of Non-Enveloped Viruses and Bacteria by an Electrically Charged Disinfectant Containing Meso-Structure Nanoparticles via Modification of the Genome

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