Expression and characterization of purinergic receptors in rat middle meningeal artery-potential role in migraine.

The dura mater and its vasculature have for decades been central in the hypothesis of migraine and headache pathophysiology. Although recent studies have questioned the role of the vasculature as the primary cause, dural vessel physiology is still relevant in understanding the complex pathophysiolog...

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Autores principales:	Kristian Agmund Haanes, Lars Edvinsson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/315558f103554c538e6a160b876d421d
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spelling	oai:doaj.org-article:315558f103554c538e6a160b876d421d2021-11-25T05:58:52ZExpression and characterization of purinergic receptors in rat middle meningeal artery-potential role in migraine.1932-620310.1371/journal.pone.0108782https://doaj.org/article/315558f103554c538e6a160b876d421d2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0108782https://doaj.org/toc/1932-6203The dura mater and its vasculature have for decades been central in the hypothesis of migraine and headache pathophysiology. Although recent studies have questioned the role of the vasculature as the primary cause, dural vessel physiology is still relevant in understanding the complex pathophysiology of migraine. The aim of the present study was to isolate the middle meningeal artery (MMA) from rodents and characterize their purinergic receptors using a sensitive wire myograph method and RT-PCR. The data presented herein suggest that blood flow through the MMA is, at least in part, regulated by purinergic receptors. P2X1 and P2Y6 receptors are the strongest contractile receptors and, surprisingly, ADPβS caused contraction most likely via P2Y1 or P2Y13 receptors, which is not observed in other arteries. Adenosine addition, however, caused relaxation of the MMA. The adenosine relaxation could be inhibited by SCH58261 (A2A receptor antagonist) and caffeine (adenosine receptor antagonist). This gives one putative molecular mechanism for the effect of caffeine, often used as an adjuvant remedy of cranial pain. Semi-quantitative RT-PCR expression data for the receptors correlate well with the functional findings. Together these observations could be used as targets for future understanding of the in vivo role of purinergic receptors in the MMA.Kristian Agmund HaanesLars EdvinssonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e108782 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kristian Agmund Haanes Lars Edvinsson Expression and characterization of purinergic receptors in rat middle meningeal artery-potential role in migraine.
description	The dura mater and its vasculature have for decades been central in the hypothesis of migraine and headache pathophysiology. Although recent studies have questioned the role of the vasculature as the primary cause, dural vessel physiology is still relevant in understanding the complex pathophysiology of migraine. The aim of the present study was to isolate the middle meningeal artery (MMA) from rodents and characterize their purinergic receptors using a sensitive wire myograph method and RT-PCR. The data presented herein suggest that blood flow through the MMA is, at least in part, regulated by purinergic receptors. P2X1 and P2Y6 receptors are the strongest contractile receptors and, surprisingly, ADPβS caused contraction most likely via P2Y1 or P2Y13 receptors, which is not observed in other arteries. Adenosine addition, however, caused relaxation of the MMA. The adenosine relaxation could be inhibited by SCH58261 (A2A receptor antagonist) and caffeine (adenosine receptor antagonist). This gives one putative molecular mechanism for the effect of caffeine, often used as an adjuvant remedy of cranial pain. Semi-quantitative RT-PCR expression data for the receptors correlate well with the functional findings. Together these observations could be used as targets for future understanding of the in vivo role of purinergic receptors in the MMA.
format	article
author	Kristian Agmund Haanes Lars Edvinsson
author_facet	Kristian Agmund Haanes Lars Edvinsson
author_sort	Kristian Agmund Haanes
title	Expression and characterization of purinergic receptors in rat middle meningeal artery-potential role in migraine.
title_short	Expression and characterization of purinergic receptors in rat middle meningeal artery-potential role in migraine.
title_full	Expression and characterization of purinergic receptors in rat middle meningeal artery-potential role in migraine.
title_fullStr	Expression and characterization of purinergic receptors in rat middle meningeal artery-potential role in migraine.
title_full_unstemmed	Expression and characterization of purinergic receptors in rat middle meningeal artery-potential role in migraine.
title_sort	expression and characterization of purinergic receptors in rat middle meningeal artery-potential role in migraine.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/315558f103554c538e6a160b876d421d
work_keys_str_mv	AT kristianagmundhaanes expressionandcharacterizationofpurinergicreceptorsinratmiddlemeningealarterypotentialroleinmigraine AT larsedvinsson expressionandcharacterizationofpurinergicreceptorsinratmiddlemeningealarterypotentialroleinmigraine
_version_	1718414330714652672

Expression and characterization of purinergic receptors in rat middle meningeal artery-potential role in migraine.

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