Fish environmental RNA enables precise ecological surveys with high positive predictivity

Metabarcoding analysis using environmental DNA (eDNA) is increasingly being used for monitoring biota. However, knowledge regarding abundance of fish environmental RNA (eRNA) and its utility in ecological survey is limited. In this study, we aimed to perform eDNA/eRNA metabarcoding analyses of fishe...

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Autores principales:	Kaede Miyata, Yasuaki Inoue, Yuto Amano, Tohru Nishioka, Masayuki Yamane, Takamitsu Kawaguchi, Osamu Morita, Hiroshi Honda
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Metabarcoding Environmental DNA Environmental RNA Sustainable development goals Traditional field surveys Ecology QH540-549.5
Acceso en línea:	https://doaj.org/article/3156b5d9a685438ebb0cebb36df22a5c
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spelling	oai:doaj.org-article:3156b5d9a685438ebb0cebb36df22a5c2021-12-01T04:53:45ZFish environmental RNA enables precise ecological surveys with high positive predictivity1470-160X10.1016/j.ecolind.2021.107796https://doaj.org/article/3156b5d9a685438ebb0cebb36df22a5c2021-09-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1470160X21004611https://doaj.org/toc/1470-160XMetabarcoding analysis using environmental DNA (eDNA) is increasingly being used for monitoring biota. However, knowledge regarding abundance of fish environmental RNA (eRNA) and its utility in ecological survey is limited. In this study, we aimed to perform eDNA/eRNA metabarcoding analyses of fishes using water samples from rivers in Japan. Sufficient amounts of eRNA from various species were present in the environmental water samples, which can support ecological survey based on eRNA metabarcoding. The sensitivity of eRNA was equivalent to that of eDNA; however, its positive predictivity was superior to that of eDNA. In addition, correlation with abundance in the ecological survey was higher for eRNA than for eDNA. Only eRNA (and the ratio of eRNA and eDNA) enabled distinguishing saltwater or brackish water fishes (false positives) that could be generated due to contamination from domestic wastewater containing their corpses or body parts or from bird feces. To the best of our knowledge, this is the first report of the possibility of eRNA to serve as a critical indicator of living biotic assemblages that actually inhabit sampling points.Kaede MiyataYasuaki InoueYuto AmanoTohru NishiokaMasayuki YamaneTakamitsu KawaguchiOsamu MoritaHiroshi HondaElsevierarticleMetabarcodingEnvironmental DNAEnvironmental RNASustainable development goalsTraditional field surveysEcologyQH540-549.5ENEcological Indicators, Vol 128, Iss , Pp 107796- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Metabarcoding Environmental DNA Environmental RNA Sustainable development goals Traditional field surveys Ecology QH540-549.5
spellingShingle	Metabarcoding Environmental DNA Environmental RNA Sustainable development goals Traditional field surveys Ecology QH540-549.5 Kaede Miyata Yasuaki Inoue Yuto Amano Tohru Nishioka Masayuki Yamane Takamitsu Kawaguchi Osamu Morita Hiroshi Honda Fish environmental RNA enables precise ecological surveys with high positive predictivity
description	Metabarcoding analysis using environmental DNA (eDNA) is increasingly being used for monitoring biota. However, knowledge regarding abundance of fish environmental RNA (eRNA) and its utility in ecological survey is limited. In this study, we aimed to perform eDNA/eRNA metabarcoding analyses of fishes using water samples from rivers in Japan. Sufficient amounts of eRNA from various species were present in the environmental water samples, which can support ecological survey based on eRNA metabarcoding. The sensitivity of eRNA was equivalent to that of eDNA; however, its positive predictivity was superior to that of eDNA. In addition, correlation with abundance in the ecological survey was higher for eRNA than for eDNA. Only eRNA (and the ratio of eRNA and eDNA) enabled distinguishing saltwater or brackish water fishes (false positives) that could be generated due to contamination from domestic wastewater containing their corpses or body parts or from bird feces. To the best of our knowledge, this is the first report of the possibility of eRNA to serve as a critical indicator of living biotic assemblages that actually inhabit sampling points.
format	article
author	Kaede Miyata Yasuaki Inoue Yuto Amano Tohru Nishioka Masayuki Yamane Takamitsu Kawaguchi Osamu Morita Hiroshi Honda
author_facet	Kaede Miyata Yasuaki Inoue Yuto Amano Tohru Nishioka Masayuki Yamane Takamitsu Kawaguchi Osamu Morita Hiroshi Honda
author_sort	Kaede Miyata
title	Fish environmental RNA enables precise ecological surveys with high positive predictivity
title_short	Fish environmental RNA enables precise ecological surveys with high positive predictivity
title_full	Fish environmental RNA enables precise ecological surveys with high positive predictivity
title_fullStr	Fish environmental RNA enables precise ecological surveys with high positive predictivity
title_full_unstemmed	Fish environmental RNA enables precise ecological surveys with high positive predictivity
title_sort	fish environmental rna enables precise ecological surveys with high positive predictivity
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/3156b5d9a685438ebb0cebb36df22a5c
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Fish environmental RNA enables precise ecological surveys with high positive predictivity

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