Long term conservation of DNA at ambient temperature. Implications for DNA data storage.

DNA conservation is central to many applications. This leads to an ever-increasing number of samples which are more and more difficult and costly to store or transport. A way to alleviate this problem is to develop procedures for storing samples at room temperature while maintaining their stability....

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Autores principales:	Delphine Coudy, Marthe Colotte, Aurélie Luis, Sophie Tuffet, Jacques Bonnet
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/315fe522ad4746d39211681b602e606d
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spelling	oai:doaj.org-article:315fe522ad4746d39211681b602e606d2021-12-02T20:07:34ZLong term conservation of DNA at ambient temperature. Implications for DNA data storage.1932-620310.1371/journal.pone.0259868https://doaj.org/article/315fe522ad4746d39211681b602e606d2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0259868https://doaj.org/toc/1932-6203DNA conservation is central to many applications. This leads to an ever-increasing number of samples which are more and more difficult and costly to store or transport. A way to alleviate this problem is to develop procedures for storing samples at room temperature while maintaining their stability. A variety of commercial systems have been proposed but they fail to completely protect DNA from deleterious factors, mainly water. On the other side, Imagene company has developed a procedure for long-term conservation of biospecimen at room temperature based on the confinement of the samples under an anhydrous and anoxic atmosphere maintained inside hermetic capsules. The procedure has been validated by us and others for purified RNA, and for DNA in buffy coat or white blood cells lysates, but a precise determination of purified DNA stability is still lacking. We used the Arrhenius law to determine the DNA degradation rate at room temperature. We found that extrapolation to 25°C gave a degradation rate constant equivalent to about 1 cut/century/100 000 nucleotides, a stability several orders of magnitude larger than the current commercialized processes. Such a stability is fundamental for many applications such as the preservation of very large DNA molecules (particularly interesting in the context of genome sequencing) or oligonucleotides for DNA data storage. Capsules are also well suited for this latter application because of their high capacity. One can calculate that the 64 zettabytes of data produced in 2020 could be stored, standalone, for centuries, in about 20 kg of capsules.Delphine CoudyMarthe ColotteAurélie LuisSophie TuffetJacques BonnetPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0259868 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Delphine Coudy Marthe Colotte Aurélie Luis Sophie Tuffet Jacques Bonnet Long term conservation of DNA at ambient temperature. Implications for DNA data storage.
description	DNA conservation is central to many applications. This leads to an ever-increasing number of samples which are more and more difficult and costly to store or transport. A way to alleviate this problem is to develop procedures for storing samples at room temperature while maintaining their stability. A variety of commercial systems have been proposed but they fail to completely protect DNA from deleterious factors, mainly water. On the other side, Imagene company has developed a procedure for long-term conservation of biospecimen at room temperature based on the confinement of the samples under an anhydrous and anoxic atmosphere maintained inside hermetic capsules. The procedure has been validated by us and others for purified RNA, and for DNA in buffy coat or white blood cells lysates, but a precise determination of purified DNA stability is still lacking. We used the Arrhenius law to determine the DNA degradation rate at room temperature. We found that extrapolation to 25°C gave a degradation rate constant equivalent to about 1 cut/century/100 000 nucleotides, a stability several orders of magnitude larger than the current commercialized processes. Such a stability is fundamental for many applications such as the preservation of very large DNA molecules (particularly interesting in the context of genome sequencing) or oligonucleotides for DNA data storage. Capsules are also well suited for this latter application because of their high capacity. One can calculate that the 64 zettabytes of data produced in 2020 could be stored, standalone, for centuries, in about 20 kg of capsules.
format	article
author	Delphine Coudy Marthe Colotte Aurélie Luis Sophie Tuffet Jacques Bonnet
author_facet	Delphine Coudy Marthe Colotte Aurélie Luis Sophie Tuffet Jacques Bonnet
author_sort	Delphine Coudy
title	Long term conservation of DNA at ambient temperature. Implications for DNA data storage.
title_short	Long term conservation of DNA at ambient temperature. Implications for DNA data storage.
title_full	Long term conservation of DNA at ambient temperature. Implications for DNA data storage.
title_fullStr	Long term conservation of DNA at ambient temperature. Implications for DNA data storage.
title_full_unstemmed	Long term conservation of DNA at ambient temperature. Implications for DNA data storage.
title_sort	long term conservation of dna at ambient temperature. implications for dna data storage.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/315fe522ad4746d39211681b602e606d
work_keys_str_mv	AT delphinecoudy longtermconservationofdnaatambienttemperatureimplicationsfordnadatastorage AT marthecolotte longtermconservationofdnaatambienttemperatureimplicationsfordnadatastorage AT aurelieluis longtermconservationofdnaatambienttemperatureimplicationsfordnadatastorage AT sophietuffet longtermconservationofdnaatambienttemperatureimplicationsfordnadatastorage AT jacquesbonnet longtermconservationofdnaatambienttemperatureimplicationsfordnadatastorage
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Long term conservation of DNA at ambient temperature. Implications for DNA data storage.

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