Disagreement between human papillomavirus assays: an unexpected challenge for the choice of an assay in primary cervical screening.

We aimed to determine the disagreement in primary cervical screening between four human papillomavirus assays: Hybrid Capture 2, cobas, CLART, and APTIMA. Material from 5,064 SurePath samples of women participating in routine cervical screening in Copenhagen, Denmark, was tested with the four assays...

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Autores principales:	Matejka Rebolj, Sarah Preisler, Ditte Møller Ejegod, Carsten Rygaard, Elsebeth Lynge, Jesper Bonde
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3160af342dcb4f6eb54102b13a5fdef5
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spelling	oai:doaj.org-article:3160af342dcb4f6eb54102b13a5fdef52021-11-18T08:37:09ZDisagreement between human papillomavirus assays: an unexpected challenge for the choice of an assay in primary cervical screening.1932-620310.1371/journal.pone.0086835https://doaj.org/article/3160af342dcb4f6eb54102b13a5fdef52014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24466262/?tool=EBIhttps://doaj.org/toc/1932-6203We aimed to determine the disagreement in primary cervical screening between four human papillomavirus assays: Hybrid Capture 2, cobas, CLART, and APTIMA. Material from 5,064 SurePath samples of women participating in routine cervical screening in Copenhagen, Denmark, was tested with the four assays. Positive agreement between the assays was measured as the conditional probability that the results of all compared assays were positive given that at least one assay returned a positive result. Of all 5,064 samples, 1,679 (33.2%) tested positive on at least one of the assays. Among these, 41% tested positive on all four. Agreement was lower in women aged ≥ 30 years (30%, vs. 49% at <30 years), in primary screening samples (29%, vs. 38% in follow-up samples), and in women with concurrent normal cytology (22%, vs. 68% with abnormal cytology). Among primary screening samples from women aged 30-65 years (n = 2,881), 23% tested positive on at least one assay, and 42 to 58% of these showed positive agreement on any compared pair of the assays. While 4% of primary screening samples showed abnormal cytology, 6 to 10% were discordant on any pair of assays. A literature review corroborated our findings of considerable disagreement between human papillomavirus assays. This suggested that the extent of disagreement in primary screening is neither population- nor storage media-specific, leaving assay design differences as the most probable cause. The substantially different selection of women testing positive on the various human papillomavirus assays represents an unexpected challenge for the choice of an assay in primary cervical screening, and for follow up of in particular HPV positive/cytology normal women.Matejka ReboljSarah PreislerDitte Møller EjegodCarsten RygaardElsebeth LyngeJesper BondePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e86835 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Matejka Rebolj Sarah Preisler Ditte Møller Ejegod Carsten Rygaard Elsebeth Lynge Jesper Bonde Disagreement between human papillomavirus assays: an unexpected challenge for the choice of an assay in primary cervical screening.
description	We aimed to determine the disagreement in primary cervical screening between four human papillomavirus assays: Hybrid Capture 2, cobas, CLART, and APTIMA. Material from 5,064 SurePath samples of women participating in routine cervical screening in Copenhagen, Denmark, was tested with the four assays. Positive agreement between the assays was measured as the conditional probability that the results of all compared assays were positive given that at least one assay returned a positive result. Of all 5,064 samples, 1,679 (33.2%) tested positive on at least one of the assays. Among these, 41% tested positive on all four. Agreement was lower in women aged ≥ 30 years (30%, vs. 49% at <30 years), in primary screening samples (29%, vs. 38% in follow-up samples), and in women with concurrent normal cytology (22%, vs. 68% with abnormal cytology). Among primary screening samples from women aged 30-65 years (n = 2,881), 23% tested positive on at least one assay, and 42 to 58% of these showed positive agreement on any compared pair of the assays. While 4% of primary screening samples showed abnormal cytology, 6 to 10% were discordant on any pair of assays. A literature review corroborated our findings of considerable disagreement between human papillomavirus assays. This suggested that the extent of disagreement in primary screening is neither population- nor storage media-specific, leaving assay design differences as the most probable cause. The substantially different selection of women testing positive on the various human papillomavirus assays represents an unexpected challenge for the choice of an assay in primary cervical screening, and for follow up of in particular HPV positive/cytology normal women.
format	article
author	Matejka Rebolj Sarah Preisler Ditte Møller Ejegod Carsten Rygaard Elsebeth Lynge Jesper Bonde
author_facet	Matejka Rebolj Sarah Preisler Ditte Møller Ejegod Carsten Rygaard Elsebeth Lynge Jesper Bonde
author_sort	Matejka Rebolj
title	Disagreement between human papillomavirus assays: an unexpected challenge for the choice of an assay in primary cervical screening.
title_short	Disagreement between human papillomavirus assays: an unexpected challenge for the choice of an assay in primary cervical screening.
title_full	Disagreement between human papillomavirus assays: an unexpected challenge for the choice of an assay in primary cervical screening.
title_fullStr	Disagreement between human papillomavirus assays: an unexpected challenge for the choice of an assay in primary cervical screening.
title_full_unstemmed	Disagreement between human papillomavirus assays: an unexpected challenge for the choice of an assay in primary cervical screening.
title_sort	disagreement between human papillomavirus assays: an unexpected challenge for the choice of an assay in primary cervical screening.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/3160af342dcb4f6eb54102b13a5fdef5
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Disagreement between human papillomavirus assays: an unexpected challenge for the choice of an assay in primary cervical screening.

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