Characterisation of lung macrophage subpopulations in COPD patients and controls

Abstract Lung macrophage subpopulations have been identified based on size. We investigated characteristics of small and large macrophages in the alveolar spaces and lung interstitium of COPD patients and controls. Alveolar and interstitial cells were isolated from lung resection tissue from 88 pati...

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Autores principales:	Jennifer A. Dewhurst, Simon Lea, Elizabeth Hardaker, Josiah V. Dungwa, Arjun K. Ravi, Dave Singh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/316b156eddba4147a7d699541ed2fda1
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spelling	oai:doaj.org-article:316b156eddba4147a7d699541ed2fda12021-12-02T11:40:59ZCharacterisation of lung macrophage subpopulations in COPD patients and controls10.1038/s41598-017-07101-22045-2322https://doaj.org/article/316b156eddba4147a7d699541ed2fda12017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07101-2https://doaj.org/toc/2045-2322Abstract Lung macrophage subpopulations have been identified based on size. We investigated characteristics of small and large macrophages in the alveolar spaces and lung interstitium of COPD patients and controls. Alveolar and interstitial cells were isolated from lung resection tissue from 88 patients. Macrophage subpopulation cell-surface expression of immunological markers and phagocytic ability were assessed by flow cytometry. Inflammatory related gene expression was measured. Alveolar and interstitial macrophages had subpopulations of small and large macrophages based on size and granularity. Alveolar macrophages had similar numbers of small and large cells; interstitial macrophages were mainly small. Small macrophages expressed significantly higher cell surface HLA-DR, CD14, CD38 and CD36 and lower CD206 compared to large macrophages. Large alveolar macrophages showed lower marker expression in COPD current compared to ex-smokers. Small interstitial macrophages had the highest pro-inflammatory gene expression levels, while large alveolar macrophages had the lowest. Small alveolar macrophages had the highest phagocytic ability. Small alveolar macrophage CD206 expression was lower in COPD patients compared to smokers. COPD lung macrophages include distinct subpopulations; Small interstitial and small alveolar macrophages with more pro-inflammatory and phagocytic function respectively, and large alveolar macrophages with low pro-inflammatory and phagocytic ability.Jennifer A. DewhurstSimon LeaElizabeth HardakerJosiah V. DungwaArjun K. RaviDave SinghNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jennifer A. Dewhurst Simon Lea Elizabeth Hardaker Josiah V. Dungwa Arjun K. Ravi Dave Singh Characterisation of lung macrophage subpopulations in COPD patients and controls
description	Abstract Lung macrophage subpopulations have been identified based on size. We investigated characteristics of small and large macrophages in the alveolar spaces and lung interstitium of COPD patients and controls. Alveolar and interstitial cells were isolated from lung resection tissue from 88 patients. Macrophage subpopulation cell-surface expression of immunological markers and phagocytic ability were assessed by flow cytometry. Inflammatory related gene expression was measured. Alveolar and interstitial macrophages had subpopulations of small and large macrophages based on size and granularity. Alveolar macrophages had similar numbers of small and large cells; interstitial macrophages were mainly small. Small macrophages expressed significantly higher cell surface HLA-DR, CD14, CD38 and CD36 and lower CD206 compared to large macrophages. Large alveolar macrophages showed lower marker expression in COPD current compared to ex-smokers. Small interstitial macrophages had the highest pro-inflammatory gene expression levels, while large alveolar macrophages had the lowest. Small alveolar macrophages had the highest phagocytic ability. Small alveolar macrophage CD206 expression was lower in COPD patients compared to smokers. COPD lung macrophages include distinct subpopulations; Small interstitial and small alveolar macrophages with more pro-inflammatory and phagocytic function respectively, and large alveolar macrophages with low pro-inflammatory and phagocytic ability.
format	article
author	Jennifer A. Dewhurst Simon Lea Elizabeth Hardaker Josiah V. Dungwa Arjun K. Ravi Dave Singh
author_facet	Jennifer A. Dewhurst Simon Lea Elizabeth Hardaker Josiah V. Dungwa Arjun K. Ravi Dave Singh
author_sort	Jennifer A. Dewhurst
title	Characterisation of lung macrophage subpopulations in COPD patients and controls
title_short	Characterisation of lung macrophage subpopulations in COPD patients and controls
title_full	Characterisation of lung macrophage subpopulations in COPD patients and controls
title_fullStr	Characterisation of lung macrophage subpopulations in COPD patients and controls
title_full_unstemmed	Characterisation of lung macrophage subpopulations in COPD patients and controls
title_sort	characterisation of lung macrophage subpopulations in copd patients and controls
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/316b156eddba4147a7d699541ed2fda1
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Characterisation of lung macrophage subpopulations in COPD patients and controls

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