Studying Gene Expression: Database Searches and Promoter Fusions to Investigate Transcriptional Regulation in Bacteria

A laboratory project was designed to illustrate how to search biological databases and utilize the information provided by these resources to investigate transcriptional regulation in Escherichia coli. The students searched several databases (NCBI Genomes, RegulonDB and EcoCyc) to learn about gene f...

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Autores principales:	Betsy M. Martinez-Vaz, Irina Makarevitch, Shane Stensland
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2010
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/316b2b451b5d4f0bb6388fbb6cc83c85
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spelling	oai:doaj.org-article:316b2b451b5d4f0bb6388fbb6cc83c852021-11-15T15:04:03ZStudying Gene Expression: Database Searches and Promoter Fusions to Investigate Transcriptional Regulation in Bacteria10.1128/jmbe.v11.i1.1011935-78851935-7877https://doaj.org/article/316b2b451b5d4f0bb6388fbb6cc83c852010-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v11.i1.101https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885A laboratory project was designed to illustrate how to search biological databases and utilize the information provided by these resources to investigate transcriptional regulation in Escherichia coli. The students searched several databases (NCBI Genomes, RegulonDB and EcoCyc) to learn about gene function, regulation, and the organization of transcriptional units. A fluorometer and GFP promoter fusions were used to obtain fluorescence data and measure changes in transcriptional activity. The class designed and performed experiments to investigate the regulation of genes necessary for biosynthesis of amino acids and how expression is affected by environmental signals and transcriptional regulators. Assessment data showed that this activity enhanced students’ knowledge of databases, reporter genes and transcriptional regulation.Betsy M. Martinez-VazIrina MakarevitchShane StenslandAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 11, Iss 1, Pp 42-49 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Betsy M. Martinez-Vaz Irina Makarevitch Shane Stensland Studying Gene Expression: Database Searches and Promoter Fusions to Investigate Transcriptional Regulation in Bacteria
description	A laboratory project was designed to illustrate how to search biological databases and utilize the information provided by these resources to investigate transcriptional regulation in Escherichia coli. The students searched several databases (NCBI Genomes, RegulonDB and EcoCyc) to learn about gene function, regulation, and the organization of transcriptional units. A fluorometer and GFP promoter fusions were used to obtain fluorescence data and measure changes in transcriptional activity. The class designed and performed experiments to investigate the regulation of genes necessary for biosynthesis of amino acids and how expression is affected by environmental signals and transcriptional regulators. Assessment data showed that this activity enhanced students’ knowledge of databases, reporter genes and transcriptional regulation.
format	article
author	Betsy M. Martinez-Vaz Irina Makarevitch Shane Stensland
author_facet	Betsy M. Martinez-Vaz Irina Makarevitch Shane Stensland
author_sort	Betsy M. Martinez-Vaz
title	Studying Gene Expression: Database Searches and Promoter Fusions to Investigate Transcriptional Regulation in Bacteria
title_short	Studying Gene Expression: Database Searches and Promoter Fusions to Investigate Transcriptional Regulation in Bacteria
title_full	Studying Gene Expression: Database Searches and Promoter Fusions to Investigate Transcriptional Regulation in Bacteria
title_fullStr	Studying Gene Expression: Database Searches and Promoter Fusions to Investigate Transcriptional Regulation in Bacteria
title_full_unstemmed	Studying Gene Expression: Database Searches and Promoter Fusions to Investigate Transcriptional Regulation in Bacteria
title_sort	studying gene expression: database searches and promoter fusions to investigate transcriptional regulation in bacteria
publisher	American Society for Microbiology
publishDate	2010
url	https://doaj.org/article/316b2b451b5d4f0bb6388fbb6cc83c85
work_keys_str_mv	AT betsymmartinezvaz studyinggeneexpressiondatabasesearchesandpromoterfusionstoinvestigatetranscriptionalregulationinbacteria AT irinamakarevitch studyinggeneexpressiondatabasesearchesandpromoterfusionstoinvestigatetranscriptionalregulationinbacteria AT shanestensland studyinggeneexpressiondatabasesearchesandpromoterfusionstoinvestigatetranscriptionalregulationinbacteria
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Studying Gene Expression: Database Searches and Promoter Fusions to Investigate Transcriptional Regulation in Bacteria

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