The Effects of Human Amniotic Membrane on Healing of Colonic Anastomosis in Dogs

Background Anastomotic leakage is claimed to be responsible for about one third of deaths following colon surgeries. Therefore, research on applied materials that may prevent leakage and improve healing requires more attention. Objectives This study was conducted to determine surgical and histo...

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Autores principales:	Neda Najibpour, Mohammad Bagher Jahantab, Massood Hosseinzadeh, Reza Roshanravan, Sam Moslemi, Salar Rahimikazerooni, Ali Reza Safarpour, Leila Ghahramani, Seyed Vahid Hosseini
Formato:	article
Lenguaje:	EN
Publicado:	Shiraz University of Medical Sciences 2013
Materias:	colon colorectal surgery biologic dressing biocompatible materials Medicine R
Acceso en línea:	https://doaj.org/article/317571605974488fb16f54d62666ebe4
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spelling	oai:doaj.org-article:317571605974488fb16f54d62666ebe42021-11-14T07:53:44ZThe Effects of Human Amniotic Membrane on Healing of Colonic Anastomosis in Dogs2783-243010.17795/acr-16139https://doaj.org/article/317571605974488fb16f54d62666ebe42013-12-01T00:00:00Zhttps://colorectalresearch.sums.ac.ir/article_45461_a6ca4b7c76f86d09dd4d3ef70f50fc32.pdfhttps://doaj.org/toc/2783-2430Background Anastomotic leakage is claimed to be responsible for about one third of deaths following colon surgeries. Therefore, research on applied materials that may prevent leakage and improve healing requires more attention. Objectives This study was conducted to determine surgical and histological outcomes of applying human amniotic membrane (HAM) in colonic anastomosis in dogs. Materials and Methods Eight cross-breed male dogs were divided into two equal groups. After anesthesia and exploration, 5cm of left colon was resected, and end-to-end anastomosis was performed in a single layer. In the treatment group (B), HAM patch measuring 2×3 cm was wrapped around the anastomotic line. Mann-Whitney U test was used to compare the results in the two groups due to small sample size, and normal distribution of data was examined using the kolmogorov-simirnov test (P = 0.03). Results Modified scoring system for surgical wound healing was used to identify the grade of healing in all samples. The healing score was significantly higher in the HAM group (P = 0.01). Conclusions HAM plays a positive role in healing of colonic anastomosis, and would lead to better histological outcomes compared to simple anastomosis in dogs.Neda NajibpourMohammad Bagher JahantabMassood HosseinzadehReza RoshanravanSam MoslemiSalar RahimikazerooniAli Reza SafarpourLeila GhahramaniSeyed Vahid HosseiniShiraz University of Medical Sciencesarticlecoloncolorectal surgerybiologic dressingbiocompatible materialsMedicineRENIranian Journal of Colorectal Research, Vol 1, Iss 3, Pp 9-1 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	colon colorectal surgery biologic dressing biocompatible materials Medicine R
spellingShingle	colon colorectal surgery biologic dressing biocompatible materials Medicine R Neda Najibpour Mohammad Bagher Jahantab Massood Hosseinzadeh Reza Roshanravan Sam Moslemi Salar Rahimikazerooni Ali Reza Safarpour Leila Ghahramani Seyed Vahid Hosseini The Effects of Human Amniotic Membrane on Healing of Colonic Anastomosis in Dogs
description	Background Anastomotic leakage is claimed to be responsible for about one third of deaths following colon surgeries. Therefore, research on applied materials that may prevent leakage and improve healing requires more attention. Objectives This study was conducted to determine surgical and histological outcomes of applying human amniotic membrane (HAM) in colonic anastomosis in dogs. Materials and Methods Eight cross-breed male dogs were divided into two equal groups. After anesthesia and exploration, 5cm of left colon was resected, and end-to-end anastomosis was performed in a single layer. In the treatment group (B), HAM patch measuring 2×3 cm was wrapped around the anastomotic line. Mann-Whitney U test was used to compare the results in the two groups due to small sample size, and normal distribution of data was examined using the kolmogorov-simirnov test (P = 0.03). Results Modified scoring system for surgical wound healing was used to identify the grade of healing in all samples. The healing score was significantly higher in the HAM group (P = 0.01). Conclusions HAM plays a positive role in healing of colonic anastomosis, and would lead to better histological outcomes compared to simple anastomosis in dogs.
format	article
author	Neda Najibpour Mohammad Bagher Jahantab Massood Hosseinzadeh Reza Roshanravan Sam Moslemi Salar Rahimikazerooni Ali Reza Safarpour Leila Ghahramani Seyed Vahid Hosseini
author_facet	Neda Najibpour Mohammad Bagher Jahantab Massood Hosseinzadeh Reza Roshanravan Sam Moslemi Salar Rahimikazerooni Ali Reza Safarpour Leila Ghahramani Seyed Vahid Hosseini
author_sort	Neda Najibpour
title	The Effects of Human Amniotic Membrane on Healing of Colonic Anastomosis in Dogs
title_short	The Effects of Human Amniotic Membrane on Healing of Colonic Anastomosis in Dogs
title_full	The Effects of Human Amniotic Membrane on Healing of Colonic Anastomosis in Dogs
title_fullStr	The Effects of Human Amniotic Membrane on Healing of Colonic Anastomosis in Dogs
title_full_unstemmed	The Effects of Human Amniotic Membrane on Healing of Colonic Anastomosis in Dogs
title_sort	effects of human amniotic membrane on healing of colonic anastomosis in dogs
publisher	Shiraz University of Medical Sciences
publishDate	2013
url	https://doaj.org/article/317571605974488fb16f54d62666ebe4
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The Effects of Human Amniotic Membrane on Healing of Colonic Anastomosis in Dogs

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