Combined laser and ozone therapy for onychomycosis in an in vitro and ex vivo model.

In order to develop a fast combined method for onychomycosis treatment using an in vitro and an ex vivo models, a combination of two dual-diode lasers at 405 nm and 639 nm wavelengths, in a continuous manner, together with different ozone concentrations (until 80 ppm), was used for performing the ex...

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Autores principales:	Javier Fernández, Iván Del Valle Fernández, Claudio J Villar, Felipe Lombó
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/317e606b7fb8460dbbd01d96aa4b2eb5
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spelling	oai:doaj.org-article:317e606b7fb8460dbbd01d96aa4b2eb52021-12-02T20:15:42ZCombined laser and ozone therapy for onychomycosis in an in vitro and ex vivo model.1932-620310.1371/journal.pone.0253979https://doaj.org/article/317e606b7fb8460dbbd01d96aa4b2eb52021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253979https://doaj.org/toc/1932-6203In order to develop a fast combined method for onychomycosis treatment using an in vitro and an ex vivo models, a combination of two dual-diode lasers at 405 nm and 639 nm wavelengths, in a continuous manner, together with different ozone concentrations (until 80 ppm), was used for performing the experiments on fungal strains growing on PDA agar medium or on pig's hooves samples. In the in vitro model experiments, with 30 min combined treatment, all species are inhibited at 40 ppm ozone concentration, except S. brevicaulis, which didn't show an inhibition in comparison with only ozone treatment. In the ex vivo model experiments, with the same duration and ozone concentration, A. chrysogenum and E. floccosum showed total inhibition; T. mentagrophytes and T. rubrum showed a 75% growth inhibition; M. canis showed a delay in sporulation; and S. brevicaulis and A. terreus did not show growth inhibition. This combined laser and ozone treatment may be developed as a fast therapy for human onychomycosis, as a potential alternative to the use of antifungal drugs with potential side effects and long duration treatments.Javier FernándezIván Del Valle FernándezClaudio J VillarFelipe LombóPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0253979 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Javier Fernández Iván Del Valle Fernández Claudio J Villar Felipe Lombó Combined laser and ozone therapy for onychomycosis in an in vitro and ex vivo model.
description	In order to develop a fast combined method for onychomycosis treatment using an in vitro and an ex vivo models, a combination of two dual-diode lasers at 405 nm and 639 nm wavelengths, in a continuous manner, together with different ozone concentrations (until 80 ppm), was used for performing the experiments on fungal strains growing on PDA agar medium or on pig's hooves samples. In the in vitro model experiments, with 30 min combined treatment, all species are inhibited at 40 ppm ozone concentration, except S. brevicaulis, which didn't show an inhibition in comparison with only ozone treatment. In the ex vivo model experiments, with the same duration and ozone concentration, A. chrysogenum and E. floccosum showed total inhibition; T. mentagrophytes and T. rubrum showed a 75% growth inhibition; M. canis showed a delay in sporulation; and S. brevicaulis and A. terreus did not show growth inhibition. This combined laser and ozone treatment may be developed as a fast therapy for human onychomycosis, as a potential alternative to the use of antifungal drugs with potential side effects and long duration treatments.
format	article
author	Javier Fernández Iván Del Valle Fernández Claudio J Villar Felipe Lombó
author_facet	Javier Fernández Iván Del Valle Fernández Claudio J Villar Felipe Lombó
author_sort	Javier Fernández
title	Combined laser and ozone therapy for onychomycosis in an in vitro and ex vivo model.
title_short	Combined laser and ozone therapy for onychomycosis in an in vitro and ex vivo model.
title_full	Combined laser and ozone therapy for onychomycosis in an in vitro and ex vivo model.
title_fullStr	Combined laser and ozone therapy for onychomycosis in an in vitro and ex vivo model.
title_full_unstemmed	Combined laser and ozone therapy for onychomycosis in an in vitro and ex vivo model.
title_sort	combined laser and ozone therapy for onychomycosis in an in vitro and ex vivo model.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/317e606b7fb8460dbbd01d96aa4b2eb5
work_keys_str_mv	AT javierfernandez combinedlaserandozonetherapyforonychomycosisinaninvitroandexvivomodel AT ivandelvallefernandez combinedlaserandozonetherapyforonychomycosisinaninvitroandexvivomodel AT claudiojvillar combinedlaserandozonetherapyforonychomycosisinaninvitroandexvivomodel AT felipelombo combinedlaserandozonetherapyforonychomycosisinaninvitroandexvivomodel
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Combined laser and ozone therapy for onychomycosis in an in vitro and ex vivo model.

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