MALDI-TOF mass spectrometry is a fast and reliable platform for identification and ecological studies of species from family Rhizobiaceae.

Family Rhizobiaceae includes fast growing bacteria currently arranged into three genera, Rhizobium, Ensifer and Shinella, that contain pathogenic, symbiotic and saprophytic species. The identification of these species is not possible on the basis of physiological or biochemical traits and should be...

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Autores principales:	Laura Ferreira, Fernando Sánchez-Juanes, Paula García-Fraile, Raúl Rivas, Pedro F Mateos, Eustoquio Martínez-Molina, José Manuel González-Buitrago, Encarna Velázquez
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3180b849826f47888a967894428af16b
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spelling	oai:doaj.org-article:3180b849826f47888a967894428af16b2021-11-18T06:52:48ZMALDI-TOF mass spectrometry is a fast and reliable platform for identification and ecological studies of species from family Rhizobiaceae.1932-620310.1371/journal.pone.0020223https://doaj.org/article/3180b849826f47888a967894428af16b2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21655291/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Family Rhizobiaceae includes fast growing bacteria currently arranged into three genera, Rhizobium, Ensifer and Shinella, that contain pathogenic, symbiotic and saprophytic species. The identification of these species is not possible on the basis of physiological or biochemical traits and should be based on sequencing of several genes. Therefore alternative methods are necessary for rapid and reliable identification of members from family Rhizobiaceae. In this work we evaluated the suitability of Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF MS) for this purpose. Firstly, we evaluated the capability of this methodology to differentiate among species of family Rhizobiaceae including those closely related and then we extended the database of MALDI Biotyper 2.0 including the type strains of 56 species from genera Rhizobium, Ensifer and Shinella. Secondly, we evaluated the identification potential of this methodology by using several strains isolated from different sources previously identified on the basis of their rrs, recA and atpD gene sequences. The 100% of these strains were correctly identified showing that MALDI-TOF MS is an excellent tool for identification of fast growing rhizobia applicable to large populations of isolates in ecological and taxonomic studies.Laura FerreiraFernando Sánchez-JuanesPaula García-FraileRaúl RivasPedro F MateosEustoquio Martínez-MolinaJosé Manuel González-BuitragoEncarna VelázquezPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 5, p e20223 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Laura Ferreira Fernando Sánchez-Juanes Paula García-Fraile Raúl Rivas Pedro F Mateos Eustoquio Martínez-Molina José Manuel González-Buitrago Encarna Velázquez MALDI-TOF mass spectrometry is a fast and reliable platform for identification and ecological studies of species from family Rhizobiaceae.
description	Family Rhizobiaceae includes fast growing bacteria currently arranged into three genera, Rhizobium, Ensifer and Shinella, that contain pathogenic, symbiotic and saprophytic species. The identification of these species is not possible on the basis of physiological or biochemical traits and should be based on sequencing of several genes. Therefore alternative methods are necessary for rapid and reliable identification of members from family Rhizobiaceae. In this work we evaluated the suitability of Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF MS) for this purpose. Firstly, we evaluated the capability of this methodology to differentiate among species of family Rhizobiaceae including those closely related and then we extended the database of MALDI Biotyper 2.0 including the type strains of 56 species from genera Rhizobium, Ensifer and Shinella. Secondly, we evaluated the identification potential of this methodology by using several strains isolated from different sources previously identified on the basis of their rrs, recA and atpD gene sequences. The 100% of these strains were correctly identified showing that MALDI-TOF MS is an excellent tool for identification of fast growing rhizobia applicable to large populations of isolates in ecological and taxonomic studies.
format	article
author	Laura Ferreira Fernando Sánchez-Juanes Paula García-Fraile Raúl Rivas Pedro F Mateos Eustoquio Martínez-Molina José Manuel González-Buitrago Encarna Velázquez
author_facet	Laura Ferreira Fernando Sánchez-Juanes Paula García-Fraile Raúl Rivas Pedro F Mateos Eustoquio Martínez-Molina José Manuel González-Buitrago Encarna Velázquez
author_sort	Laura Ferreira
title	MALDI-TOF mass spectrometry is a fast and reliable platform for identification and ecological studies of species from family Rhizobiaceae.
title_short	MALDI-TOF mass spectrometry is a fast and reliable platform for identification and ecological studies of species from family Rhizobiaceae.
title_full	MALDI-TOF mass spectrometry is a fast and reliable platform for identification and ecological studies of species from family Rhizobiaceae.
title_fullStr	MALDI-TOF mass spectrometry is a fast and reliable platform for identification and ecological studies of species from family Rhizobiaceae.
title_full_unstemmed	MALDI-TOF mass spectrometry is a fast and reliable platform for identification and ecological studies of species from family Rhizobiaceae.
title_sort	maldi-tof mass spectrometry is a fast and reliable platform for identification and ecological studies of species from family rhizobiaceae.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/3180b849826f47888a967894428af16b
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MALDI-TOF mass spectrometry is a fast and reliable platform for identification and ecological studies of species from family Rhizobiaceae.

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