Affinity Purification Coupled to Stable Isotope Dilution LC-MS/MS Analysis to Discover IgG4 Glycosylation Profiles for Autoimmune Pancreatitis

Type 1 autoimmune pancreatitis (AIP) is categorized as an IgG4-related disease (IgG4-RD), where a high concentration of plasma IgG4 is one of the common biomarkers among patients. IgG Fc-glycosylation has been reported to be potential biosignatures for diseases. However, human IgG3 and IgG4 Fc-glyco...

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Autores principales:	Michael X. Chen, Ho-Hsuan Su, Ching-Ya Shiao, Yu-Ting Chang, Ming-Chu Chang, Chih-Chin Kao, San-Yuan Wang, Hsi-Chang Shih, I-Lin Tsai
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	type 1 autoimmune pancreatitis IgG4 N-glycosylation mass spectrometry Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/3187c85526884c67925b6259ef5b0d60
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spelling	oai:doaj.org-article:3187c85526884c67925b6259ef5b0d602021-11-11T16:59:13ZAffinity Purification Coupled to Stable Isotope Dilution LC-MS/MS Analysis to Discover IgG4 Glycosylation Profiles for Autoimmune Pancreatitis10.3390/ijms2221115271422-00671661-6596https://doaj.org/article/3187c85526884c67925b6259ef5b0d602021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11527https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Type 1 autoimmune pancreatitis (AIP) is categorized as an IgG4-related disease (IgG4-RD), where a high concentration of plasma IgG4 is one of the common biomarkers among patients. IgG Fc-glycosylation has been reported to be potential biosignatures for diseases. However, human IgG3 and IgG4 Fc-glycopeptides from populations in Asia were found to be isobaric ions when using LC-MS/MS as an analytical tool. In this study, an analytical workflow that coupled affinity purification and stable isotope dilution LC-MS/MS was developed to dissect IgG4 glycosylation profiles for autoimmune pancreatitis. Comparing the IgG4 and glycosylation profiles among healthy controls, patients with pancreatic ductal adenocarcinoma (PDAC), and AIP, the IgG4 glycosylations from the AIP group were found to have more digalactosylation (compared to PDAC) and less monogalactosylation (compared to HC). In addition, higher fucosylation and sialylation profiles were also discovered for the AIP group. The workflow is efficient and selective for IgG4 glycopeptides, and can be used for clinical biosignature discovery.Michael X. ChenHo-Hsuan SuChing-Ya ShiaoYu-Ting ChangMing-Chu ChangChih-Chin KaoSan-Yuan WangHsi-Chang ShihI-Lin TsaiMDPI AGarticletype 1 autoimmune pancreatitisIgG4N-glycosylationmass spectrometryBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11527, p 11527 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	type 1 autoimmune pancreatitis IgG4 N-glycosylation mass spectrometry Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	type 1 autoimmune pancreatitis IgG4 N-glycosylation mass spectrometry Biology (General) QH301-705.5 Chemistry QD1-999 Michael X. Chen Ho-Hsuan Su Ching-Ya Shiao Yu-Ting Chang Ming-Chu Chang Chih-Chin Kao San-Yuan Wang Hsi-Chang Shih I-Lin Tsai Affinity Purification Coupled to Stable Isotope Dilution LC-MS/MS Analysis to Discover IgG4 Glycosylation Profiles for Autoimmune Pancreatitis
description	Type 1 autoimmune pancreatitis (AIP) is categorized as an IgG4-related disease (IgG4-RD), where a high concentration of plasma IgG4 is one of the common biomarkers among patients. IgG Fc-glycosylation has been reported to be potential biosignatures for diseases. However, human IgG3 and IgG4 Fc-glycopeptides from populations in Asia were found to be isobaric ions when using LC-MS/MS as an analytical tool. In this study, an analytical workflow that coupled affinity purification and stable isotope dilution LC-MS/MS was developed to dissect IgG4 glycosylation profiles for autoimmune pancreatitis. Comparing the IgG4 and glycosylation profiles among healthy controls, patients with pancreatic ductal adenocarcinoma (PDAC), and AIP, the IgG4 glycosylations from the AIP group were found to have more digalactosylation (compared to PDAC) and less monogalactosylation (compared to HC). In addition, higher fucosylation and sialylation profiles were also discovered for the AIP group. The workflow is efficient and selective for IgG4 glycopeptides, and can be used for clinical biosignature discovery.
format	article
author	Michael X. Chen Ho-Hsuan Su Ching-Ya Shiao Yu-Ting Chang Ming-Chu Chang Chih-Chin Kao San-Yuan Wang Hsi-Chang Shih I-Lin Tsai
author_facet	Michael X. Chen Ho-Hsuan Su Ching-Ya Shiao Yu-Ting Chang Ming-Chu Chang Chih-Chin Kao San-Yuan Wang Hsi-Chang Shih I-Lin Tsai
author_sort	Michael X. Chen
title	Affinity Purification Coupled to Stable Isotope Dilution LC-MS/MS Analysis to Discover IgG4 Glycosylation Profiles for Autoimmune Pancreatitis
title_short	Affinity Purification Coupled to Stable Isotope Dilution LC-MS/MS Analysis to Discover IgG4 Glycosylation Profiles for Autoimmune Pancreatitis
title_full	Affinity Purification Coupled to Stable Isotope Dilution LC-MS/MS Analysis to Discover IgG4 Glycosylation Profiles for Autoimmune Pancreatitis
title_fullStr	Affinity Purification Coupled to Stable Isotope Dilution LC-MS/MS Analysis to Discover IgG4 Glycosylation Profiles for Autoimmune Pancreatitis
title_full_unstemmed	Affinity Purification Coupled to Stable Isotope Dilution LC-MS/MS Analysis to Discover IgG4 Glycosylation Profiles for Autoimmune Pancreatitis
title_sort	affinity purification coupled to stable isotope dilution lc-ms/ms analysis to discover igg4 glycosylation profiles for autoimmune pancreatitis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3187c85526884c67925b6259ef5b0d60
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Affinity Purification Coupled to Stable Isotope Dilution LC-MS/MS Analysis to Discover IgG4 Glycosylation Profiles for Autoimmune Pancreatitis

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