Rapid monoisotopic cisplatin based barcoding for multiplexed mass cytometry

Abstract Mass cytometry presents an exceptional opportunity to interrogate the biology of highly heterogeneous cell populations, owing to the ability to collect highly parametric proteomic data at a single cell level. However, sample-to-sample variability, due to antibody staining and/or instrument...

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Autores principales:	Ryan L. McCarthy, Duncan H. Mak, Jared K. Burks, Michelle C. Barton
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3189a0ba7a0641888ff626cfe63e61ed
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spelling	oai:doaj.org-article:3189a0ba7a0641888ff626cfe63e61ed2021-12-02T16:06:26ZRapid monoisotopic cisplatin based barcoding for multiplexed mass cytometry10.1038/s41598-017-03610-22045-2322https://doaj.org/article/3189a0ba7a0641888ff626cfe63e61ed2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03610-2https://doaj.org/toc/2045-2322Abstract Mass cytometry presents an exceptional opportunity to interrogate the biology of highly heterogeneous cell populations, owing to the ability to collect highly parametric proteomic data at a single cell level. However, sample-to-sample variability, due to antibody staining and/or instrument sensitivity, can introduce substantial artifacts into the data, which can in turn lead to erroneous conclusions. This variability can be eliminated by sample barcoding which enables samples to be pooled, stained and run simultaneously. Existing mass cytometry barcoding approaches require time intensive labeling, reduce the number of biologically meaningful parameters and/or rely on expensive reagents. We present an approach utilizing monoisotopic cisplatin to perform cell barcoding that does not require cell permeabilization, can be completed in 10 minutes and can be utilized in combination with existing barcoding techniques to greatly increase the number of samples which can be multiplexed to improve throughput and consistency.Ryan L. McCarthyDuncan H. MakJared K. BurksMichelle C. BartonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-6 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ryan L. McCarthy Duncan H. Mak Jared K. Burks Michelle C. Barton Rapid monoisotopic cisplatin based barcoding for multiplexed mass cytometry
description	Abstract Mass cytometry presents an exceptional opportunity to interrogate the biology of highly heterogeneous cell populations, owing to the ability to collect highly parametric proteomic data at a single cell level. However, sample-to-sample variability, due to antibody staining and/or instrument sensitivity, can introduce substantial artifacts into the data, which can in turn lead to erroneous conclusions. This variability can be eliminated by sample barcoding which enables samples to be pooled, stained and run simultaneously. Existing mass cytometry barcoding approaches require time intensive labeling, reduce the number of biologically meaningful parameters and/or rely on expensive reagents. We present an approach utilizing monoisotopic cisplatin to perform cell barcoding that does not require cell permeabilization, can be completed in 10 minutes and can be utilized in combination with existing barcoding techniques to greatly increase the number of samples which can be multiplexed to improve throughput and consistency.
format	article
author	Ryan L. McCarthy Duncan H. Mak Jared K. Burks Michelle C. Barton
author_facet	Ryan L. McCarthy Duncan H. Mak Jared K. Burks Michelle C. Barton
author_sort	Ryan L. McCarthy
title	Rapid monoisotopic cisplatin based barcoding for multiplexed mass cytometry
title_short	Rapid monoisotopic cisplatin based barcoding for multiplexed mass cytometry
title_full	Rapid monoisotopic cisplatin based barcoding for multiplexed mass cytometry
title_fullStr	Rapid monoisotopic cisplatin based barcoding for multiplexed mass cytometry
title_full_unstemmed	Rapid monoisotopic cisplatin based barcoding for multiplexed mass cytometry
title_sort	rapid monoisotopic cisplatin based barcoding for multiplexed mass cytometry
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3189a0ba7a0641888ff626cfe63e61ed
work_keys_str_mv	AT ryanlmccarthy rapidmonoisotopiccisplatinbasedbarcodingformultiplexedmasscytometry AT duncanhmak rapidmonoisotopiccisplatinbasedbarcodingformultiplexedmasscytometry AT jaredkburks rapidmonoisotopiccisplatinbasedbarcodingformultiplexedmasscytometry AT michellecbarton rapidmonoisotopiccisplatinbasedbarcodingformultiplexedmasscytometry
_version_	1718384953588187136

Rapid monoisotopic cisplatin based barcoding for multiplexed mass cytometry

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