Graphene oxide scaffold accelerates cellular proliferative response and alveolar bone healing of tooth extraction socket

Erika Nishida,1 Hirofumi Miyaji,1 Akihito Kato,1 Hiroko Takita,2 Toshihiko Iwanaga,3 Takehito Momose,1 Kosuke Ogawa,1 Shusuke Murakami,1 Tsutomu Sugaya,1 Masamitsu Kawanami11Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan; 2Suppo...

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Autores principales:	Nishida E, Miyaji H, Kato A, Takita H, Iwanaga T, Momose T, Ogawa K, Murakami S, Sugaya T, Kawanami M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Biocompatibility biomaterial bone tissue engineering cell ingrowth collagen sponge macrophage nanocarbon Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3191d2ac1d7043bea99f565446d8b799
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spelling	oai:doaj.org-article:3191d2ac1d7043bea99f565446d8b7992021-12-02T01:50:41ZGraphene oxide scaffold accelerates cellular proliferative response and alveolar bone healing of tooth extraction socket1178-2013https://doaj.org/article/3191d2ac1d7043bea99f565446d8b7992016-05-01T00:00:00Zhttps://www.dovepress.com/graphene-oxide-scaffold-accelerates-cellular-proliferative-response-an-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Erika Nishida,1 Hirofumi Miyaji,1 Akihito Kato,1 Hiroko Takita,2 Toshihiko Iwanaga,3 Takehito Momose,1 Kosuke Ogawa,1 Shusuke Murakami,1 Tsutomu Sugaya,1 Masamitsu Kawanami11Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan; 2Support Section for Education and Research, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan; 3Laboratory of Histology and Cytology, Hokkaido University Graduate School of Medicine, Sapporo, JapanAbstract: Graphene oxide (GO) consisting of a carbon monolayer has been widely investigated for tissue engineering platforms because of its unique properties. For this study, we fabricated a GO-applied scaffold and assessed the cellular and tissue behaviors in the scaffold. A preclinical test was conducted to ascertain whether the GO scaffold promoted bone induction in dog tooth extraction sockets. For this study, GO scaffolds were prepared by coating the surface of a collagen sponge scaffold with 0.1 and 1 µg/mL GO dispersion. Scaffolds were characterized using scanning electron microscopy (SEM), physical testing, cell seeding, and rat subcutaneous implant testing. Then a GO scaffold was implanted into a dog tooth extraction socket. Histological observations were made at 2 weeks postsurgery. SEM observations show that GO attached to the surface of collagen scaffold struts. The GO scaffold exhibited an interconnected structure resembling that of control subjects. GO application improved the physical strength, enzyme resistance, and adsorption of calcium and proteins. Cytocompatibility tests showed that GO application significantly increased osteoblastic MC3T3-E1 cell proliferation. In addition, an assessment of rat subcutaneous tissue response revealed that implantation of 1 µg/mL GO scaffold stimulated cellular ingrowth behavior, suggesting that the GO scaffold exhibited good biocompatibility. The tissue ingrowth area and DNA contents of 1 µg/mL GO scaffold were, respectively, approximately 2.5-fold and 1.4-fold greater than those of the control. Particularly, the infiltration of ED2-positive (M2) macrophages and blood vessels were prominent in the GO scaffold. Dog bone-formation tests showed that 1 µg/mL GO scaffold implantation enhanced bone formation. New bone formation following GO scaffold implantation was enhanced fivefold compared to that in control subjects. These results suggest that GO was biocompatible and had high bone-formation capability for the scaffold. The GO scaffold is expected to be beneficial for bone tissue engineering therapy.Keywords: biocompatibility, biomaterial, bone tissue engineering, cell ingrowth, collagen sponge, macrophage, nanocarbonNishida EMiyaji HKato ATakita HIwanaga TMomose TOgawa KMurakami SSugaya TKawanami MDove Medical PressarticleBiocompatibilitybiomaterialbone tissue engineeringcell ingrowthcollagen spongemacrophagenanocarbonMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 2265-2277 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biocompatibility biomaterial bone tissue engineering cell ingrowth collagen sponge macrophage nanocarbon Medicine (General) R5-920
spellingShingle	Biocompatibility biomaterial bone tissue engineering cell ingrowth collagen sponge macrophage nanocarbon Medicine (General) R5-920 Nishida E Miyaji H Kato A Takita H Iwanaga T Momose T Ogawa K Murakami S Sugaya T Kawanami M Graphene oxide scaffold accelerates cellular proliferative response and alveolar bone healing of tooth extraction socket
description	Erika Nishida,1 Hirofumi Miyaji,1 Akihito Kato,1 Hiroko Takita,2 Toshihiko Iwanaga,3 Takehito Momose,1 Kosuke Ogawa,1 Shusuke Murakami,1 Tsutomu Sugaya,1 Masamitsu Kawanami11Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan; 2Support Section for Education and Research, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan; 3Laboratory of Histology and Cytology, Hokkaido University Graduate School of Medicine, Sapporo, JapanAbstract: Graphene oxide (GO) consisting of a carbon monolayer has been widely investigated for tissue engineering platforms because of its unique properties. For this study, we fabricated a GO-applied scaffold and assessed the cellular and tissue behaviors in the scaffold. A preclinical test was conducted to ascertain whether the GO scaffold promoted bone induction in dog tooth extraction sockets. For this study, GO scaffolds were prepared by coating the surface of a collagen sponge scaffold with 0.1 and 1 µg/mL GO dispersion. Scaffolds were characterized using scanning electron microscopy (SEM), physical testing, cell seeding, and rat subcutaneous implant testing. Then a GO scaffold was implanted into a dog tooth extraction socket. Histological observations were made at 2 weeks postsurgery. SEM observations show that GO attached to the surface of collagen scaffold struts. The GO scaffold exhibited an interconnected structure resembling that of control subjects. GO application improved the physical strength, enzyme resistance, and adsorption of calcium and proteins. Cytocompatibility tests showed that GO application significantly increased osteoblastic MC3T3-E1 cell proliferation. In addition, an assessment of rat subcutaneous tissue response revealed that implantation of 1 µg/mL GO scaffold stimulated cellular ingrowth behavior, suggesting that the GO scaffold exhibited good biocompatibility. The tissue ingrowth area and DNA contents of 1 µg/mL GO scaffold were, respectively, approximately 2.5-fold and 1.4-fold greater than those of the control. Particularly, the infiltration of ED2-positive (M2) macrophages and blood vessels were prominent in the GO scaffold. Dog bone-formation tests showed that 1 µg/mL GO scaffold implantation enhanced bone formation. New bone formation following GO scaffold implantation was enhanced fivefold compared to that in control subjects. These results suggest that GO was biocompatible and had high bone-formation capability for the scaffold. The GO scaffold is expected to be beneficial for bone tissue engineering therapy.Keywords: biocompatibility, biomaterial, bone tissue engineering, cell ingrowth, collagen sponge, macrophage, nanocarbon
format	article
author	Nishida E Miyaji H Kato A Takita H Iwanaga T Momose T Ogawa K Murakami S Sugaya T Kawanami M
author_facet	Nishida E Miyaji H Kato A Takita H Iwanaga T Momose T Ogawa K Murakami S Sugaya T Kawanami M
author_sort	Nishida E
title	Graphene oxide scaffold accelerates cellular proliferative response and alveolar bone healing of tooth extraction socket
title_short	Graphene oxide scaffold accelerates cellular proliferative response and alveolar bone healing of tooth extraction socket
title_full	Graphene oxide scaffold accelerates cellular proliferative response and alveolar bone healing of tooth extraction socket
title_fullStr	Graphene oxide scaffold accelerates cellular proliferative response and alveolar bone healing of tooth extraction socket
title_full_unstemmed	Graphene oxide scaffold accelerates cellular proliferative response and alveolar bone healing of tooth extraction socket
title_sort	graphene oxide scaffold accelerates cellular proliferative response and alveolar bone healing of tooth extraction socket
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/3191d2ac1d7043bea99f565446d8b799
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Graphene oxide scaffold accelerates cellular proliferative response and alveolar bone healing of tooth extraction socket

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