Development of a duplex real-time PCR method for the detection of influenza C and D viruses

Abstract Influenza viruses are major respiratory pathogens known to infect human and a variety of animals and are widely prevalent worldwide. Genome structure of influenza D virus (IDV) is identical to that of influenza C virus (ICV), and phylogenetic analyses suggest that IDV and ICV share a common...

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Autores principales:	Letian Zhang, Meng Lu, Jiaxuan Lu, Ningning Wang, Zhongzhou Pan, Shuo Su
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Influenza C virus Influenza D virus Real-time PCR Multiplex detection Veterinary medicine SF600-1100 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/31b3ba3d9e71435eb6e8e66208cd188d
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spelling	oai:doaj.org-article:31b3ba3d9e71435eb6e8e66208cd188d2021-11-14T12:06:12ZDevelopment of a duplex real-time PCR method for the detection of influenza C and D viruses10.1186/s44149-021-00016-62731-0442https://doaj.org/article/31b3ba3d9e71435eb6e8e66208cd188d2021-09-01T00:00:00Zhttps://doi.org/10.1186/s44149-021-00016-6https://doaj.org/toc/2731-0442Abstract Influenza viruses are major respiratory pathogens known to infect human and a variety of animals and are widely prevalent worldwide. Genome structure of influenza D virus (IDV) is identical to that of influenza C virus (ICV), and phylogenetic analyses suggest that IDV and ICV share a common ancestry and high homology. To date, the prevalence of ICV and IDV in China is unclear, but these viruses represent a potential threat to public health due to cross-species transmission and zoonotic potential. To efficiently monitor ICV and IDV, it is necessary to establish a dual detection method to understand their prevalence and conduct in-depth research. A duplex real-time PCR method for the simultaneous detection of ICV and IDV was developed. TaqMan fluorescent probes and specific primers targeting NP gene of ICV and PB1 gene of IDV were designed. This method exhibited good specificity and sensitivity, and the detection limit reached 1 × 101 copies/μL of plasmid standards of each pathogen. Thirty-one clinical swine samples and 10 clinical cattle samples were analyzed using this method. One positive sample of IDV was detected, and the accuracy of clinical test results was verified by conventional PCR and DNA sequencing. The duplex real-time PCR detection method represents a sensitive and specific tool to detect ICV and IDV. It provides technical support for virus research and clinical diagnosis of ICV and IDV. This information will benefit animal and human health.Letian ZhangMeng LuJiaxuan LuNingning WangZhongzhou PanShuo SuBMCarticleInfluenza C virusInfluenza D virusReal-time PCRMultiplex detectionVeterinary medicineSF600-1100Public aspects of medicineRA1-1270ENAnimal Diseases, Vol 1, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Influenza C virus Influenza D virus Real-time PCR Multiplex detection Veterinary medicine SF600-1100 Public aspects of medicine RA1-1270
spellingShingle	Influenza C virus Influenza D virus Real-time PCR Multiplex detection Veterinary medicine SF600-1100 Public aspects of medicine RA1-1270 Letian Zhang Meng Lu Jiaxuan Lu Ningning Wang Zhongzhou Pan Shuo Su Development of a duplex real-time PCR method for the detection of influenza C and D viruses
description	Abstract Influenza viruses are major respiratory pathogens known to infect human and a variety of animals and are widely prevalent worldwide. Genome structure of influenza D virus (IDV) is identical to that of influenza C virus (ICV), and phylogenetic analyses suggest that IDV and ICV share a common ancestry and high homology. To date, the prevalence of ICV and IDV in China is unclear, but these viruses represent a potential threat to public health due to cross-species transmission and zoonotic potential. To efficiently monitor ICV and IDV, it is necessary to establish a dual detection method to understand their prevalence and conduct in-depth research. A duplex real-time PCR method for the simultaneous detection of ICV and IDV was developed. TaqMan fluorescent probes and specific primers targeting NP gene of ICV and PB1 gene of IDV were designed. This method exhibited good specificity and sensitivity, and the detection limit reached 1 × 101 copies/μL of plasmid standards of each pathogen. Thirty-one clinical swine samples and 10 clinical cattle samples were analyzed using this method. One positive sample of IDV was detected, and the accuracy of clinical test results was verified by conventional PCR and DNA sequencing. The duplex real-time PCR detection method represents a sensitive and specific tool to detect ICV and IDV. It provides technical support for virus research and clinical diagnosis of ICV and IDV. This information will benefit animal and human health.
format	article
author	Letian Zhang Meng Lu Jiaxuan Lu Ningning Wang Zhongzhou Pan Shuo Su
author_facet	Letian Zhang Meng Lu Jiaxuan Lu Ningning Wang Zhongzhou Pan Shuo Su
author_sort	Letian Zhang
title	Development of a duplex real-time PCR method for the detection of influenza C and D viruses
title_short	Development of a duplex real-time PCR method for the detection of influenza C and D viruses
title_full	Development of a duplex real-time PCR method for the detection of influenza C and D viruses
title_fullStr	Development of a duplex real-time PCR method for the detection of influenza C and D viruses
title_full_unstemmed	Development of a duplex real-time PCR method for the detection of influenza C and D viruses
title_sort	development of a duplex real-time pcr method for the detection of influenza c and d viruses
publisher	BMC
publishDate	2021
url	https://doaj.org/article/31b3ba3d9e71435eb6e8e66208cd188d
work_keys_str_mv	AT letianzhang developmentofaduplexrealtimepcrmethodforthedetectionofinfluenzacanddviruses AT menglu developmentofaduplexrealtimepcrmethodforthedetectionofinfluenzacanddviruses AT jiaxuanlu developmentofaduplexrealtimepcrmethodforthedetectionofinfluenzacanddviruses AT ningningwang developmentofaduplexrealtimepcrmethodforthedetectionofinfluenzacanddviruses AT zhongzhoupan developmentofaduplexrealtimepcrmethodforthedetectionofinfluenzacanddviruses AT shuosu developmentofaduplexrealtimepcrmethodforthedetectionofinfluenzacanddviruses
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Development of a duplex real-time PCR method for the detection of influenza C and D viruses

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