Detection of hepatitis C virus core protein in serum by atomic force microscopy combined with mass spectrometry

Detection of hepatitis C virus core protein in serum by atomic force microscopy combined with mass spectrometry

Yuri D Ivanov,1 Anna L Kaysheva,1,2 Pavel A Frantsuzov,1 Tatyana O Pleshakova,1 Nikolay V Krohin,1 Alexander A Izotov,1 Ivan D Shumov,1 Vasiliy F Uchaikin,1 Vladimir A Konev,1 Vadim S Ziborov,1 Alexander I Archakov11Institute of Biomedical Chemistry, 2PostgenTech Ltd, Moscow, RussiaAbstract: A meth...

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Autores principales: Ivanov YD, Kaysheva AL, Frantsuzov PA, Pleshakova TO, Krohin NV, Izotov AA, Shumov ID, Uchaikin VF, Konev VA, Ziborov VS, Archakov AI
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2015
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Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/31b70a4b96e94b319fc9907cc4723f45
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spelling oai:doaj.org-article:31b70a4b96e94b319fc9907cc4723f452021-12-02T00:13:00ZDetection of hepatitis C virus core protein in serum by atomic force microscopy combined with mass spectrometry1178-2013https://doaj.org/article/31b70a4b96e94b319fc9907cc4723f452015-02-01T00:00:00Zhttp://www.dovepress.com/detection-of-hepatitis-c-virus-core-protein-in-serum-by-atomic-force-m-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Yuri D Ivanov,1 Anna L Kaysheva,1,2 Pavel A Frantsuzov,1 Tatyana O Pleshakova,1 Nikolay V Krohin,1 Alexander A Izotov,1 Ivan D Shumov,1 Vasiliy F Uchaikin,1 Vladimir A Konev,1 Vadim S Ziborov,1 Alexander I Archakov11Institute of Biomedical Chemistry, 2PostgenTech Ltd, Moscow, RussiaAbstract: A method for detection and identification of core antigen of hepatitis C virus (HCVcoreAg)-containing particles in the serum was proposed, with due account taken of the interactions of proteotypic peptides with Na+, K+, and Cl- ions. The method is based on a combination of reversible biospecific atomic force microscopy (AFM)-fishing and mass spectrometry (MS). AFM-fishing enables concentration, detection, and counting of protein complexes captured on the AFM chip surface, with their subsequent MS identification. Biospecific AFM-fishing of HCVcoreAg-containing particles from serum samples was carried out using AFM chips with immobilized antibodies against HCVcoreAg (HCVcoreAgim). Formation of complexes between anti-HCVcoreAgim and HCVcoreAg-containing particles on the AFM chip surface during the fishing process was demonstrated. These complexes were registered and counted by AFM. Further MS analysis allowed reliable identification of HCVcoreAg within the complexes formed on the AFM chip surface. It was shown that MS data processing, with account taken of the interactions between HCVcoreAg peptides and Na+, K+ cations, and Cl- anions, allows an increase in the number of peptides identified.Keywords: hepatitis C virus, molecular detector, biospecific fishingIvanov YDKaysheva ALFrantsuzov PAPleshakova TOKrohin NVIzotov AAShumov IDUchaikin VFKonev VAZiborov VSArchakov AIDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 1597-1608 (2015)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Ivanov YD
Kaysheva AL
Frantsuzov PA
Pleshakova TO
Krohin NV
Izotov AA
Shumov ID
Uchaikin VF
Konev VA
Ziborov VS
Archakov AI
Detection of hepatitis C virus core protein in serum by atomic force microscopy combined with mass spectrometry
description Yuri D Ivanov,1 Anna L Kaysheva,1,2 Pavel A Frantsuzov,1 Tatyana O Pleshakova,1 Nikolay V Krohin,1 Alexander A Izotov,1 Ivan D Shumov,1 Vasiliy F Uchaikin,1 Vladimir A Konev,1 Vadim S Ziborov,1 Alexander I Archakov11Institute of Biomedical Chemistry, 2PostgenTech Ltd, Moscow, RussiaAbstract: A method for detection and identification of core antigen of hepatitis C virus (HCVcoreAg)-containing particles in the serum was proposed, with due account taken of the interactions of proteotypic peptides with Na+, K+, and Cl- ions. The method is based on a combination of reversible biospecific atomic force microscopy (AFM)-fishing and mass spectrometry (MS). AFM-fishing enables concentration, detection, and counting of protein complexes captured on the AFM chip surface, with their subsequent MS identification. Biospecific AFM-fishing of HCVcoreAg-containing particles from serum samples was carried out using AFM chips with immobilized antibodies against HCVcoreAg (HCVcoreAgim). Formation of complexes between anti-HCVcoreAgim and HCVcoreAg-containing particles on the AFM chip surface during the fishing process was demonstrated. These complexes were registered and counted by AFM. Further MS analysis allowed reliable identification of HCVcoreAg within the complexes formed on the AFM chip surface. It was shown that MS data processing, with account taken of the interactions between HCVcoreAg peptides and Na+, K+ cations, and Cl- anions, allows an increase in the number of peptides identified.Keywords: hepatitis C virus, molecular detector, biospecific fishing
format article
author Ivanov YD
Kaysheva AL
Frantsuzov PA
Pleshakova TO
Krohin NV
Izotov AA
Shumov ID
Uchaikin VF
Konev VA
Ziborov VS
Archakov AI
author_facet Ivanov YD
Kaysheva AL
Frantsuzov PA
Pleshakova TO
Krohin NV
Izotov AA
Shumov ID
Uchaikin VF
Konev VA
Ziborov VS
Archakov AI
author_sort Ivanov YD
title Detection of hepatitis C virus core protein in serum by atomic force microscopy combined with mass spectrometry
title_short Detection of hepatitis C virus core protein in serum by atomic force microscopy combined with mass spectrometry
title_full Detection of hepatitis C virus core protein in serum by atomic force microscopy combined with mass spectrometry
title_fullStr Detection of hepatitis C virus core protein in serum by atomic force microscopy combined with mass spectrometry
title_full_unstemmed Detection of hepatitis C virus core protein in serum by atomic force microscopy combined with mass spectrometry
title_sort detection of hepatitis c virus core protein in serum by atomic force microscopy combined with mass spectrometry
publisher Dove Medical Press
publishDate 2015
url https://doaj.org/article/31b70a4b96e94b319fc9907cc4723f45
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