Phenotypic and functional changes in blood monocytes following adherence to endothelium.

<h4>Objective</h4>Blood monocytes are known to express endothelial-like genes during co-culture with endothelium. In this study, the time-dependent change in the phenotype pattern of primary blood monocytes after adhering to endothelium is reported using a novel HLA-A2 mistyped co-cultur...

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Autores principales:	Colin Tso, Kerry-Anne Rye, Philip Barter
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/31bca63631a54fa28060358269dadb60
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spelling	oai:doaj.org-article:31bca63631a54fa28060358269dadb602021-11-18T07:18:38ZPhenotypic and functional changes in blood monocytes following adherence to endothelium.1932-620310.1371/journal.pone.0037091https://doaj.org/article/31bca63631a54fa28060358269dadb602012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22615904/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Objective</h4>Blood monocytes are known to express endothelial-like genes during co-culture with endothelium. In this study, the time-dependent change in the phenotype pattern of primary blood monocytes after adhering to endothelium is reported using a novel HLA-A2 mistyped co-culture model.<h4>Methods and results</h4>Freshly isolated human PBMCs were co-cultured with human umbilical vein endothelial cells or human coronary arterial endothelial cells of converse human leukocyte antigen A2 (HLA-A2) status. This allows the tracking of the PBMC-derived cells by HLA-A2 expression and assessment of their phenotype pattern over time. PBMCs that adhered to the endothelium at the start of the co-culture were predominantly CD11b+ blood monocytes. After 24 to 72 hours in co-culture, the endothelium-adherent monocytes acquired endothelial-like properties including the expression of endothelial nitric oxide synthase, CD105, CD144 and vascular endothelial growth factor receptor 2. The expression of monocyte/macrophage lineage antigens CD14, CD11b and CD36 were down regulated concomitantly. The adherent monocytes did not express CD115 after 1 day of co-culture. By day 6, the monocyte-derived cells expressed vascular cell adhesion molecule 1 in response to tumour necrosis factor alpha. Up to 10% of the PBMCs adhered to the endothelium. These monocyte-derived cells contributed up to 30% of the co-cultured cell layer and this was dose-dependent on the PBMC seeding density.<h4>Conclusions</h4>Human blood monocytes undergo rapid phenotype change to resemble endothelial cells after adhering to endothelium.Colin TsoKerry-Anne RyePhilip BarterPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e37091 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Colin Tso Kerry-Anne Rye Philip Barter Phenotypic and functional changes in blood monocytes following adherence to endothelium.
description	<h4>Objective</h4>Blood monocytes are known to express endothelial-like genes during co-culture with endothelium. In this study, the time-dependent change in the phenotype pattern of primary blood monocytes after adhering to endothelium is reported using a novel HLA-A2 mistyped co-culture model.<h4>Methods and results</h4>Freshly isolated human PBMCs were co-cultured with human umbilical vein endothelial cells or human coronary arterial endothelial cells of converse human leukocyte antigen A2 (HLA-A2) status. This allows the tracking of the PBMC-derived cells by HLA-A2 expression and assessment of their phenotype pattern over time. PBMCs that adhered to the endothelium at the start of the co-culture were predominantly CD11b+ blood monocytes. After 24 to 72 hours in co-culture, the endothelium-adherent monocytes acquired endothelial-like properties including the expression of endothelial nitric oxide synthase, CD105, CD144 and vascular endothelial growth factor receptor 2. The expression of monocyte/macrophage lineage antigens CD14, CD11b and CD36 were down regulated concomitantly. The adherent monocytes did not express CD115 after 1 day of co-culture. By day 6, the monocyte-derived cells expressed vascular cell adhesion molecule 1 in response to tumour necrosis factor alpha. Up to 10% of the PBMCs adhered to the endothelium. These monocyte-derived cells contributed up to 30% of the co-cultured cell layer and this was dose-dependent on the PBMC seeding density.<h4>Conclusions</h4>Human blood monocytes undergo rapid phenotype change to resemble endothelial cells after adhering to endothelium.
format	article
author	Colin Tso Kerry-Anne Rye Philip Barter
author_facet	Colin Tso Kerry-Anne Rye Philip Barter
author_sort	Colin Tso
title	Phenotypic and functional changes in blood monocytes following adherence to endothelium.
title_short	Phenotypic and functional changes in blood monocytes following adherence to endothelium.
title_full	Phenotypic and functional changes in blood monocytes following adherence to endothelium.
title_fullStr	Phenotypic and functional changes in blood monocytes following adherence to endothelium.
title_full_unstemmed	Phenotypic and functional changes in blood monocytes following adherence to endothelium.
title_sort	phenotypic and functional changes in blood monocytes following adherence to endothelium.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/31bca63631a54fa28060358269dadb60
work_keys_str_mv	AT colintso phenotypicandfunctionalchangesinbloodmonocytesfollowingadherencetoendothelium AT kerryannerye phenotypicandfunctionalchangesinbloodmonocytesfollowingadherencetoendothelium AT philipbarter phenotypicandfunctionalchangesinbloodmonocytesfollowingadherencetoendothelium
_version_	1718423654979600384

Phenotypic and functional changes in blood monocytes following adherence to endothelium.

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