The mitochondrion-like organelle of Trimastix pyriformis contains the complete glycine cleavage system.

All eukaryotic organisms contain mitochondria or organelles that evolved from the same endosymbiotic event like classical mitochondria. Organisms inhabiting low oxygen environments often contain mitochondrial derivates known as hydrogenosomes, mitosomes or neutrally as mitochondrion-like organelles....

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Autores principales:	Zuzana Zubáčová, Lukáš Novák, Jitka Bublíková, Vojtěch Vacek, Jan Fousek, Jakub Rídl, Jan Tachezy, Pavel Doležal, Cestmír Vlček, Vladimír Hampl
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/31cbff153cdf4e27abb950fe855366bd
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spelling	oai:doaj.org-article:31cbff153cdf4e27abb950fe855366bd2021-11-18T07:53:44ZThe mitochondrion-like organelle of Trimastix pyriformis contains the complete glycine cleavage system.1932-620310.1371/journal.pone.0055417https://doaj.org/article/31cbff153cdf4e27abb950fe855366bd2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23516392/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203All eukaryotic organisms contain mitochondria or organelles that evolved from the same endosymbiotic event like classical mitochondria. Organisms inhabiting low oxygen environments often contain mitochondrial derivates known as hydrogenosomes, mitosomes or neutrally as mitochondrion-like organelles. The detailed investigation has shown unexpected evolutionary plasticity in the biochemistry and protein composition of these organelles in various protists. We investigated the mitochondrion-like organelle in Trimastix pyriformis, a free-living member of one of the three lineages of anaerobic group Metamonada. Using 454 sequencing we have obtained 7 037 contigs from its transcriptome and on the basis of sequence homology and presence of N-terminal extensions we have selected contigs coding for proteins that putatively function in the organelle. Together with the results of a previous transcriptome survey, the list now consists of 23 proteins - mostly enzymes involved in amino acid metabolism, transporters and maturases of proteins and transporters of metabolites. We have no evidence of the production of ATP in the mitochondrion-like organelle of Trimastix but we have obtained experimental evidence for the presence of enzymes of the glycine cleavage system (GCS), which is part of amino acid metabolism. Using homologous antibody we have shown that H-protein of GCS localizes into vesicles in the cell of Trimastix. When overexpressed in yeast, H- and P-protein of GCS and cpn60 were transported into mitochondrion. In case of H-protein we have demonstrated that the first 16 amino acids are necessary for this transport. Glycine cleavage system is at the moment the only experimentally localized pathway in the mitochondrial derivate of Trimastix pyriformis.Zuzana ZubáčováLukáš NovákJitka BublíkováVojtěch VacekJan FousekJakub RídlJan TachezyPavel DoležalCestmír VlčekVladimír HamplPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e55417 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zuzana Zubáčová Lukáš Novák Jitka Bublíková Vojtěch Vacek Jan Fousek Jakub Rídl Jan Tachezy Pavel Doležal Cestmír Vlček Vladimír Hampl The mitochondrion-like organelle of Trimastix pyriformis contains the complete glycine cleavage system.
description	All eukaryotic organisms contain mitochondria or organelles that evolved from the same endosymbiotic event like classical mitochondria. Organisms inhabiting low oxygen environments often contain mitochondrial derivates known as hydrogenosomes, mitosomes or neutrally as mitochondrion-like organelles. The detailed investigation has shown unexpected evolutionary plasticity in the biochemistry and protein composition of these organelles in various protists. We investigated the mitochondrion-like organelle in Trimastix pyriformis, a free-living member of one of the three lineages of anaerobic group Metamonada. Using 454 sequencing we have obtained 7 037 contigs from its transcriptome and on the basis of sequence homology and presence of N-terminal extensions we have selected contigs coding for proteins that putatively function in the organelle. Together with the results of a previous transcriptome survey, the list now consists of 23 proteins - mostly enzymes involved in amino acid metabolism, transporters and maturases of proteins and transporters of metabolites. We have no evidence of the production of ATP in the mitochondrion-like organelle of Trimastix but we have obtained experimental evidence for the presence of enzymes of the glycine cleavage system (GCS), which is part of amino acid metabolism. Using homologous antibody we have shown that H-protein of GCS localizes into vesicles in the cell of Trimastix. When overexpressed in yeast, H- and P-protein of GCS and cpn60 were transported into mitochondrion. In case of H-protein we have demonstrated that the first 16 amino acids are necessary for this transport. Glycine cleavage system is at the moment the only experimentally localized pathway in the mitochondrial derivate of Trimastix pyriformis.
format	article
author	Zuzana Zubáčová Lukáš Novák Jitka Bublíková Vojtěch Vacek Jan Fousek Jakub Rídl Jan Tachezy Pavel Doležal Cestmír Vlček Vladimír Hampl
author_facet	Zuzana Zubáčová Lukáš Novák Jitka Bublíková Vojtěch Vacek Jan Fousek Jakub Rídl Jan Tachezy Pavel Doležal Cestmír Vlček Vladimír Hampl
author_sort	Zuzana Zubáčová
title	The mitochondrion-like organelle of Trimastix pyriformis contains the complete glycine cleavage system.
title_short	The mitochondrion-like organelle of Trimastix pyriformis contains the complete glycine cleavage system.
title_full	The mitochondrion-like organelle of Trimastix pyriformis contains the complete glycine cleavage system.
title_fullStr	The mitochondrion-like organelle of Trimastix pyriformis contains the complete glycine cleavage system.
title_full_unstemmed	The mitochondrion-like organelle of Trimastix pyriformis contains the complete glycine cleavage system.
title_sort	mitochondrion-like organelle of trimastix pyriformis contains the complete glycine cleavage system.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/31cbff153cdf4e27abb950fe855366bd
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The mitochondrion-like organelle of Trimastix pyriformis contains the complete glycine cleavage system.

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