Running worms: C. elegans self-sorting by electrotaxis.

The nematode C. elegans displays complex dynamical behaviors that are commonly used to identify relevant phenotypes. Although its maintenance is straightforward, sorting large populations of worms when looking for a behavioral phenotype is difficult, time consuming and hardly quantitative when done...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Xavier Manière, Félix Lebois, Ivan Matic, Benoit Ladoux, Jean-Marc Di Meglio, Pascal Hersen
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2011
Materias:
R
Q
Acceso en línea:https://doaj.org/article/31ce581c898b4910823407221ed77496
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:31ce581c898b4910823407221ed77496
record_format dspace
spelling oai:doaj.org-article:31ce581c898b4910823407221ed774962021-11-18T06:59:14ZRunning worms: C. elegans self-sorting by electrotaxis.1932-620310.1371/journal.pone.0016637https://doaj.org/article/31ce581c898b4910823407221ed774962011-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21326598/?tool=EBIhttps://doaj.org/toc/1932-6203The nematode C. elegans displays complex dynamical behaviors that are commonly used to identify relevant phenotypes. Although its maintenance is straightforward, sorting large populations of worms when looking for a behavioral phenotype is difficult, time consuming and hardly quantitative when done manually. Interestingly, when submitted to a moderate electric field, worms move steadily along straight trajectories. Here, we report an inexpensive method to measure worms crawling velocities and sort them within a few minutes by taking advantage of their electrotactic skills. This method allows to quantitatively measure the effect of mutations and aging on worm's crawling velocity. We also show that worms with different locomotory phenotypes can be spatially sorted, fast worms traveling away from slow ones. Group of nematodes with comparable locomotory fitness could then be isolated for further analysis. C. elegans is a growing model for neurodegenerative diseases and using electrotaxis for self-sorting can improve the high-throughput search of therapeutic bio-molecules.Xavier ManièreFélix LeboisIvan MaticBenoit LadouxJean-Marc Di MeglioPascal HersenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 2, p e16637 (2011)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Xavier Manière
Félix Lebois
Ivan Matic
Benoit Ladoux
Jean-Marc Di Meglio
Pascal Hersen
Running worms: C. elegans self-sorting by electrotaxis.
description The nematode C. elegans displays complex dynamical behaviors that are commonly used to identify relevant phenotypes. Although its maintenance is straightforward, sorting large populations of worms when looking for a behavioral phenotype is difficult, time consuming and hardly quantitative when done manually. Interestingly, when submitted to a moderate electric field, worms move steadily along straight trajectories. Here, we report an inexpensive method to measure worms crawling velocities and sort them within a few minutes by taking advantage of their electrotactic skills. This method allows to quantitatively measure the effect of mutations and aging on worm's crawling velocity. We also show that worms with different locomotory phenotypes can be spatially sorted, fast worms traveling away from slow ones. Group of nematodes with comparable locomotory fitness could then be isolated for further analysis. C. elegans is a growing model for neurodegenerative diseases and using electrotaxis for self-sorting can improve the high-throughput search of therapeutic bio-molecules.
format article
author Xavier Manière
Félix Lebois
Ivan Matic
Benoit Ladoux
Jean-Marc Di Meglio
Pascal Hersen
author_facet Xavier Manière
Félix Lebois
Ivan Matic
Benoit Ladoux
Jean-Marc Di Meglio
Pascal Hersen
author_sort Xavier Manière
title Running worms: C. elegans self-sorting by electrotaxis.
title_short Running worms: C. elegans self-sorting by electrotaxis.
title_full Running worms: C. elegans self-sorting by electrotaxis.
title_fullStr Running worms: C. elegans self-sorting by electrotaxis.
title_full_unstemmed Running worms: C. elegans self-sorting by electrotaxis.
title_sort running worms: c. elegans self-sorting by electrotaxis.
publisher Public Library of Science (PLoS)
publishDate 2011
url https://doaj.org/article/31ce581c898b4910823407221ed77496
work_keys_str_mv AT xaviermaniere runningwormscelegansselfsortingbyelectrotaxis
AT felixlebois runningwormscelegansselfsortingbyelectrotaxis
AT ivanmatic runningwormscelegansselfsortingbyelectrotaxis
AT benoitladoux runningwormscelegansselfsortingbyelectrotaxis
AT jeanmarcdimeglio runningwormscelegansselfsortingbyelectrotaxis
AT pascalhersen runningwormscelegansselfsortingbyelectrotaxis
_version_ 1718424092958261248