Hetero/Homo-Complexes of Sucrose Transporters May Be a Subtle Mode to Regulate Sucrose Transportation in Grape Berries

The sugar distribution mechanism in fruits has been the focus of research worldwide; however, it remains unclear. In order to elucidate the relevant mechanisms in grape berries, the expression, localization, function, and regulation of three sucrose transporters were studied in three representative...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yumeng Cai, Ling Yin, Jie Wang, Wenjie Dong, Han Gao, Jinxu Xu, Zhefang Deng, Wenrui Tu, Jing Yan, Qingyong Meng, Yali Zhang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	different varieties <i>Vitis</i> sucrose transporter functional variation oligomerization functional regulation Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/31d53ab3089745dd9d20ffcbdb00e522
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:31d53ab3089745dd9d20ffcbdb00e522
record_format	dspace
spelling	oai:doaj.org-article:31d53ab3089745dd9d20ffcbdb00e5222021-11-11T17:27:32ZHetero/Homo-Complexes of Sucrose Transporters May Be a Subtle Mode to Regulate Sucrose Transportation in Grape Berries10.3390/ijms2221120621422-00671661-6596https://doaj.org/article/31d53ab3089745dd9d20ffcbdb00e5222021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/12062https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The sugar distribution mechanism in fruits has been the focus of research worldwide; however, it remains unclear. In order to elucidate the relevant mechanisms in grape berries, the expression, localization, function, and regulation of three sucrose transporters were studied in three representative <i>Vitis</i> varieties. Both <i>SUC11</i> and <i>SUC12</i> expression levels were positively correlated with sugar accumulation in grape berries, whereas <i>SUC27</i> showed a negative relationship. The alignment analysis and sucrose transport ability of isolated SUCs were determined to reflect coding region variations among <i>V. vinifera</i>, <i>V. amurensis</i> Ruper, and <i>V. riparia</i>, indicating that functional variation existed in one SUT from different varieties. Furthermore, potentially oligomerized abilities of VvSUCs colocalized in the sieve elements of the phloem as plasma membrane proteins were verified. The effects of oligomerization on transport properties were characterized in yeast. VvSUC11 and VvSUC12 are high-affinity/low-capacity types of SUTs that stimulate each other by upregulating <i>V<sub>max</sub></i> and <i>K<sub>m</sub></i>, inhibiting sucrose transport, and downregulating the <i>K<sub>m</sub></i> of VvSUC27. Thus, changes in the distribution of different SUTs in the same cell govern functional regulation. The activation and inhibition of sucrose transport could be achieved in different stages and tissues of grape development to achieve an effective distribution of sugar.Yumeng CaiLing YinJie WangWenjie DongHan GaoJinxu XuZhefang DengWenrui TuJing YanQingyong MengYali ZhangMDPI AGarticledifferent varieties<i>Vitis</i>sucrose transporterfunctional variationoligomerizationfunctional regulationBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12062, p 12062 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	different varieties <i>Vitis</i> sucrose transporter functional variation oligomerization functional regulation Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	different varieties <i>Vitis</i> sucrose transporter functional variation oligomerization functional regulation Biology (General) QH301-705.5 Chemistry QD1-999 Yumeng Cai Ling Yin Jie Wang Wenjie Dong Han Gao Jinxu Xu Zhefang Deng Wenrui Tu Jing Yan Qingyong Meng Yali Zhang Hetero/Homo-Complexes of Sucrose Transporters May Be a Subtle Mode to Regulate Sucrose Transportation in Grape Berries
description	The sugar distribution mechanism in fruits has been the focus of research worldwide; however, it remains unclear. In order to elucidate the relevant mechanisms in grape berries, the expression, localization, function, and regulation of three sucrose transporters were studied in three representative <i>Vitis</i> varieties. Both <i>SUC11</i> and <i>SUC12</i> expression levels were positively correlated with sugar accumulation in grape berries, whereas <i>SUC27</i> showed a negative relationship. The alignment analysis and sucrose transport ability of isolated SUCs were determined to reflect coding region variations among <i>V. vinifera</i>, <i>V. amurensis</i> Ruper, and <i>V. riparia</i>, indicating that functional variation existed in one SUT from different varieties. Furthermore, potentially oligomerized abilities of VvSUCs colocalized in the sieve elements of the phloem as plasma membrane proteins were verified. The effects of oligomerization on transport properties were characterized in yeast. VvSUC11 and VvSUC12 are high-affinity/low-capacity types of SUTs that stimulate each other by upregulating <i>V<sub>max</sub></i> and <i>K<sub>m</sub></i>, inhibiting sucrose transport, and downregulating the <i>K<sub>m</sub></i> of VvSUC27. Thus, changes in the distribution of different SUTs in the same cell govern functional regulation. The activation and inhibition of sucrose transport could be achieved in different stages and tissues of grape development to achieve an effective distribution of sugar.
format	article
author	Yumeng Cai Ling Yin Jie Wang Wenjie Dong Han Gao Jinxu Xu Zhefang Deng Wenrui Tu Jing Yan Qingyong Meng Yali Zhang
author_facet	Yumeng Cai Ling Yin Jie Wang Wenjie Dong Han Gao Jinxu Xu Zhefang Deng Wenrui Tu Jing Yan Qingyong Meng Yali Zhang
author_sort	Yumeng Cai
title	Hetero/Homo-Complexes of Sucrose Transporters May Be a Subtle Mode to Regulate Sucrose Transportation in Grape Berries
title_short	Hetero/Homo-Complexes of Sucrose Transporters May Be a Subtle Mode to Regulate Sucrose Transportation in Grape Berries
title_full	Hetero/Homo-Complexes of Sucrose Transporters May Be a Subtle Mode to Regulate Sucrose Transportation in Grape Berries
title_fullStr	Hetero/Homo-Complexes of Sucrose Transporters May Be a Subtle Mode to Regulate Sucrose Transportation in Grape Berries
title_full_unstemmed	Hetero/Homo-Complexes of Sucrose Transporters May Be a Subtle Mode to Regulate Sucrose Transportation in Grape Berries
title_sort	hetero/homo-complexes of sucrose transporters may be a subtle mode to regulate sucrose transportation in grape berries
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/31d53ab3089745dd9d20ffcbdb00e522
work_keys_str_mv	AT yumengcai heterohomocomplexesofsucrosetransportersmaybeasubtlemodetoregulatesucrosetransportationingrapeberries AT lingyin heterohomocomplexesofsucrosetransportersmaybeasubtlemodetoregulatesucrosetransportationingrapeberries AT jiewang heterohomocomplexesofsucrosetransportersmaybeasubtlemodetoregulatesucrosetransportationingrapeberries AT wenjiedong heterohomocomplexesofsucrosetransportersmaybeasubtlemodetoregulatesucrosetransportationingrapeberries AT hangao heterohomocomplexesofsucrosetransportersmaybeasubtlemodetoregulatesucrosetransportationingrapeberries AT jinxuxu heterohomocomplexesofsucrosetransportersmaybeasubtlemodetoregulatesucrosetransportationingrapeberries AT zhefangdeng heterohomocomplexesofsucrosetransportersmaybeasubtlemodetoregulatesucrosetransportationingrapeberries AT wenruitu heterohomocomplexesofsucrosetransportersmaybeasubtlemodetoregulatesucrosetransportationingrapeberries AT jingyan heterohomocomplexesofsucrosetransportersmaybeasubtlemodetoregulatesucrosetransportationingrapeberries AT qingyongmeng heterohomocomplexesofsucrosetransportersmaybeasubtlemodetoregulatesucrosetransportationingrapeberries AT yalizhang heterohomocomplexesofsucrosetransportersmaybeasubtlemodetoregulatesucrosetransportationingrapeberries
_version_	1718432054244278272

Hetero/Homo-Complexes of Sucrose Transporters May Be a Subtle Mode to Regulate Sucrose Transportation in Grape Berries

Ejemplares similares