Transcriptional host–pathogen responses of Pseudogymnoascus destructans and three species of bats with white-nose syndrome

Understanding how context (e.g., host species, environmental conditions) drives disease susceptibility is an essential goal of disease ecology. We hypothesized that in bat white-nose syndrome (WNS), species-specific host–pathogen interactions may partly explain varying disease outcomes among host sp...

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Autores principales:	Christina M. Davy, Michael E. Donaldson, Hana Bandouchova, Ana M. Breit, Nicole A.S. Dorville, Yvonne A. Dzal, Veronika Kovacova, Emma L. Kunkel, Natália Martínková, Kaleigh J.O. Norquay, James E. Paterson, Jan Zukal, Jiri Pikula, Craig K.R. Willis, Christopher J. Kyle
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	disease ecology emerging infectious diseases eptesicus fuscus host–pathogen interactions susceptibility myotis lucifugus myotis myotis pseudogymnoascus destructans virulence Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/31d6d8da25d74487a9d892a80bc6c7ff
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spelling	oai:doaj.org-article:31d6d8da25d74487a9d892a80bc6c7ff2021-11-17T14:21:58ZTranscriptional host–pathogen responses of Pseudogymnoascus destructans and three species of bats with white-nose syndrome2150-55942150-560810.1080/21505594.2020.1768018https://doaj.org/article/31d6d8da25d74487a9d892a80bc6c7ff2020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1768018https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Understanding how context (e.g., host species, environmental conditions) drives disease susceptibility is an essential goal of disease ecology. We hypothesized that in bat white-nose syndrome (WNS), species-specific host–pathogen interactions may partly explain varying disease outcomes among host species. We characterized bat and pathogen transcriptomes in paired samples of lesion-positive and lesion-negative wing tissue from bats infected with Pseudogymnoascus destructans in three parallel experiments. The first two experiments analyzed samples collected from the susceptible Nearctic Myotis lucifugus and the less-susceptible Nearctic Eptesicus fuscus, following experimental infection and hibernation in captivity under controlled conditions. The third experiment applied the same analyses to paired samples from infected, free-ranging Myotis myotis, a less susceptible, Palearctic species, following natural infection and hibernation (n = 8 sample pairs/species). Gene expression by P. destructans was similar among the three host species despite varying environmental conditions among the three experiments and was similar within each host species between saprophytic contexts (superficial growth on wings) and pathogenic contexts (growth in lesions on the same wings). In contrast, we observed qualitative variation in host response: M. lucifugus and M. myotis exhibited systemic responses to infection, while E. fuscus up-regulated a remarkably localized response. Our results suggest potential phylogenetic determinants of response to WNS and can inform further studies of context-dependent host–pathogen interactions.Christina M. DavyMichael E. DonaldsonHana BandouchovaAna M. BreitNicole A.S. DorvilleYvonne A. DzalVeronika KovacovaEmma L. KunkelNatália MartínkováKaleigh J.O. NorquayJames E. PatersonJan ZukalJiri PikulaCraig K.R. WillisChristopher J. KyleTaylor & Francis Grouparticledisease ecologyemerging infectious diseaseseptesicus fuscushost–pathogen interactionssusceptibilitymyotis lucifugusmyotis myotispseudogymnoascus destructansvirulenceInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 781-794 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	disease ecology emerging infectious diseases eptesicus fuscus host–pathogen interactions susceptibility myotis lucifugus myotis myotis pseudogymnoascus destructans virulence Infectious and parasitic diseases RC109-216
spellingShingle	disease ecology emerging infectious diseases eptesicus fuscus host–pathogen interactions susceptibility myotis lucifugus myotis myotis pseudogymnoascus destructans virulence Infectious and parasitic diseases RC109-216 Christina M. Davy Michael E. Donaldson Hana Bandouchova Ana M. Breit Nicole A.S. Dorville Yvonne A. Dzal Veronika Kovacova Emma L. Kunkel Natália Martínková Kaleigh J.O. Norquay James E. Paterson Jan Zukal Jiri Pikula Craig K.R. Willis Christopher J. Kyle Transcriptional host–pathogen responses of Pseudogymnoascus destructans and three species of bats with white-nose syndrome
description	Understanding how context (e.g., host species, environmental conditions) drives disease susceptibility is an essential goal of disease ecology. We hypothesized that in bat white-nose syndrome (WNS), species-specific host–pathogen interactions may partly explain varying disease outcomes among host species. We characterized bat and pathogen transcriptomes in paired samples of lesion-positive and lesion-negative wing tissue from bats infected with Pseudogymnoascus destructans in three parallel experiments. The first two experiments analyzed samples collected from the susceptible Nearctic Myotis lucifugus and the less-susceptible Nearctic Eptesicus fuscus, following experimental infection and hibernation in captivity under controlled conditions. The third experiment applied the same analyses to paired samples from infected, free-ranging Myotis myotis, a less susceptible, Palearctic species, following natural infection and hibernation (n = 8 sample pairs/species). Gene expression by P. destructans was similar among the three host species despite varying environmental conditions among the three experiments and was similar within each host species between saprophytic contexts (superficial growth on wings) and pathogenic contexts (growth in lesions on the same wings). In contrast, we observed qualitative variation in host response: M. lucifugus and M. myotis exhibited systemic responses to infection, while E. fuscus up-regulated a remarkably localized response. Our results suggest potential phylogenetic determinants of response to WNS and can inform further studies of context-dependent host–pathogen interactions.
format	article
author	Christina M. Davy Michael E. Donaldson Hana Bandouchova Ana M. Breit Nicole A.S. Dorville Yvonne A. Dzal Veronika Kovacova Emma L. Kunkel Natália Martínková Kaleigh J.O. Norquay James E. Paterson Jan Zukal Jiri Pikula Craig K.R. Willis Christopher J. Kyle
author_facet	Christina M. Davy Michael E. Donaldson Hana Bandouchova Ana M. Breit Nicole A.S. Dorville Yvonne A. Dzal Veronika Kovacova Emma L. Kunkel Natália Martínková Kaleigh J.O. Norquay James E. Paterson Jan Zukal Jiri Pikula Craig K.R. Willis Christopher J. Kyle
author_sort	Christina M. Davy
title	Transcriptional host–pathogen responses of Pseudogymnoascus destructans and three species of bats with white-nose syndrome
title_short	Transcriptional host–pathogen responses of Pseudogymnoascus destructans and three species of bats with white-nose syndrome
title_full	Transcriptional host–pathogen responses of Pseudogymnoascus destructans and three species of bats with white-nose syndrome
title_fullStr	Transcriptional host–pathogen responses of Pseudogymnoascus destructans and three species of bats with white-nose syndrome
title_full_unstemmed	Transcriptional host–pathogen responses of Pseudogymnoascus destructans and three species of bats with white-nose syndrome
title_sort	transcriptional host–pathogen responses of pseudogymnoascus destructans and three species of bats with white-nose syndrome
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/31d6d8da25d74487a9d892a80bc6c7ff
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Transcriptional host–pathogen responses of Pseudogymnoascus destructans and three species of bats with white-nose syndrome

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