Kinetics of antibody response in critically ill patients with Middle East respiratory syndrome and association with mortality and viral clearance
Abstract The objective of this study is to examine the IgG antibody response in critically ill patients with the Middle East respiratory syndrome (MERS) and to examine the association of early antibody response with mortality and viral clearance. We collected blood samples from 40 consecutive real-t...
Guardado en:
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/31ebb17872554cefac52f3953e1ff156 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:31ebb17872554cefac52f3953e1ff156 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:31ebb17872554cefac52f3953e1ff1562021-11-21T12:17:20ZKinetics of antibody response in critically ill patients with Middle East respiratory syndrome and association with mortality and viral clearance10.1038/s41598-021-01083-y2045-2322https://doaj.org/article/31ebb17872554cefac52f3953e1ff1562021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01083-yhttps://doaj.org/toc/2045-2322Abstract The objective of this study is to examine the IgG antibody response in critically ill patients with the Middle East respiratory syndrome (MERS) and to examine the association of early antibody response with mortality and viral clearance. We collected blood samples from 40 consecutive real-time reverse transcription-polymerase chain reaction (rRT-PCR) confirmed critically ill MERS patients on ICU days 1, 3, 7, 14 and 28. MERS-CoV antibodies were detected by enzyme-linked immunosorbent assay (ELISA), using wells coated with MERS-CoV S1 antigen. Patients were admitted to ICU after a median (Q1, Q3) of 9 (4, 13) days from onset of symptoms with an admission Sequential Organ Failure Assessment (SOFA) score of 11 (6.5, 12). Among the study cohort, 38 patients (95%) received invasive ventilation, 35 (88%) vasopressors, 21 (53%) renal replacement therapy and 17 (43%) corticosteroids. Median (Q1,Q3) ELISA optical density (OD) ratio significantly increased with time (p < 0.001) from 0.11 (0.07, 1.43) on day 1; to 0.69 (0.11, 2.08) on day 3, 2.72 (1.84, 3.54) on day 7, 2.51 (0.35, 3.35) on day 14 and 3.77 (3.70, 3.84) on day 28. Early antibody response (day 1–3) was observed in 13/39 patients (33%) and was associated with lower mortality (hazard ratio: 0.31, 95% CI 0.10, 0.96, p = 0.04) but was not associated with faster clearance of MERS-CoV RNA. In conclusion, among critically ill patients with MERS, early antibody response was associated with lower mortality but not with faster clearance of MERS-CoV RNA. These findings have important implications for understanding pathogenesis and potential immunotherapy.Yaseen M ArabiAli H. HajeerHanan BalkhySameera Al JohaniMusharaf SadatAbdulaziz Al-DawoodAlanoud Abu TalebJesna JoseEman Al QasimAbdulaziz Al AjlanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Yaseen M Arabi Ali H. Hajeer Hanan Balkhy Sameera Al Johani Musharaf Sadat Abdulaziz Al-Dawood Alanoud Abu Taleb Jesna Jose Eman Al Qasim Abdulaziz Al Ajlan Kinetics of antibody response in critically ill patients with Middle East respiratory syndrome and association with mortality and viral clearance |
| description |
Abstract The objective of this study is to examine the IgG antibody response in critically ill patients with the Middle East respiratory syndrome (MERS) and to examine the association of early antibody response with mortality and viral clearance. We collected blood samples from 40 consecutive real-time reverse transcription-polymerase chain reaction (rRT-PCR) confirmed critically ill MERS patients on ICU days 1, 3, 7, 14 and 28. MERS-CoV antibodies were detected by enzyme-linked immunosorbent assay (ELISA), using wells coated with MERS-CoV S1 antigen. Patients were admitted to ICU after a median (Q1, Q3) of 9 (4, 13) days from onset of symptoms with an admission Sequential Organ Failure Assessment (SOFA) score of 11 (6.5, 12). Among the study cohort, 38 patients (95%) received invasive ventilation, 35 (88%) vasopressors, 21 (53%) renal replacement therapy and 17 (43%) corticosteroids. Median (Q1,Q3) ELISA optical density (OD) ratio significantly increased with time (p < 0.001) from 0.11 (0.07, 1.43) on day 1; to 0.69 (0.11, 2.08) on day 3, 2.72 (1.84, 3.54) on day 7, 2.51 (0.35, 3.35) on day 14 and 3.77 (3.70, 3.84) on day 28. Early antibody response (day 1–3) was observed in 13/39 patients (33%) and was associated with lower mortality (hazard ratio: 0.31, 95% CI 0.10, 0.96, p = 0.04) but was not associated with faster clearance of MERS-CoV RNA. In conclusion, among critically ill patients with MERS, early antibody response was associated with lower mortality but not with faster clearance of MERS-CoV RNA. These findings have important implications for understanding pathogenesis and potential immunotherapy. |
| format |
article |
| author |
Yaseen M Arabi Ali H. Hajeer Hanan Balkhy Sameera Al Johani Musharaf Sadat Abdulaziz Al-Dawood Alanoud Abu Taleb Jesna Jose Eman Al Qasim Abdulaziz Al Ajlan |
| author_facet |
Yaseen M Arabi Ali H. Hajeer Hanan Balkhy Sameera Al Johani Musharaf Sadat Abdulaziz Al-Dawood Alanoud Abu Taleb Jesna Jose Eman Al Qasim Abdulaziz Al Ajlan |
| author_sort |
Yaseen M Arabi |
| title |
Kinetics of antibody response in critically ill patients with Middle East respiratory syndrome and association with mortality and viral clearance |
| title_short |
Kinetics of antibody response in critically ill patients with Middle East respiratory syndrome and association with mortality and viral clearance |
| title_full |
Kinetics of antibody response in critically ill patients with Middle East respiratory syndrome and association with mortality and viral clearance |
| title_fullStr |
Kinetics of antibody response in critically ill patients with Middle East respiratory syndrome and association with mortality and viral clearance |
| title_full_unstemmed |
Kinetics of antibody response in critically ill patients with Middle East respiratory syndrome and association with mortality and viral clearance |
| title_sort |
kinetics of antibody response in critically ill patients with middle east respiratory syndrome and association with mortality and viral clearance |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/31ebb17872554cefac52f3953e1ff156 |
| work_keys_str_mv |
AT yaseenmarabi kineticsofantibodyresponseincriticallyillpatientswithmiddleeastrespiratorysyndromeandassociationwithmortalityandviralclearance AT alihhajeer kineticsofantibodyresponseincriticallyillpatientswithmiddleeastrespiratorysyndromeandassociationwithmortalityandviralclearance AT hananbalkhy kineticsofantibodyresponseincriticallyillpatientswithmiddleeastrespiratorysyndromeandassociationwithmortalityandviralclearance AT sameeraaljohani kineticsofantibodyresponseincriticallyillpatientswithmiddleeastrespiratorysyndromeandassociationwithmortalityandviralclearance AT musharafsadat kineticsofantibodyresponseincriticallyillpatientswithmiddleeastrespiratorysyndromeandassociationwithmortalityandviralclearance AT abdulazizaldawood kineticsofantibodyresponseincriticallyillpatientswithmiddleeastrespiratorysyndromeandassociationwithmortalityandviralclearance AT alanoudabutaleb kineticsofantibodyresponseincriticallyillpatientswithmiddleeastrespiratorysyndromeandassociationwithmortalityandviralclearance AT jesnajose kineticsofantibodyresponseincriticallyillpatientswithmiddleeastrespiratorysyndromeandassociationwithmortalityandviralclearance AT emanalqasim kineticsofantibodyresponseincriticallyillpatientswithmiddleeastrespiratorysyndromeandassociationwithmortalityandviralclearance AT abdulazizalajlan kineticsofantibodyresponseincriticallyillpatientswithmiddleeastrespiratorysyndromeandassociationwithmortalityandviralclearance |
| _version_ |
1718419055039217664 |