In silico drug-like properties prediction and in vivo antifungal potentials of Citrullus lanatus seed oil against Candida albicans

Citrullus lanatus seed is an important but neglected seed rich in essential fatty acids. The study sought to investigate in silico and in vivo antifungal activity of some bioactive compounds of Citrullus lanatus (watermelon) seed oil (CLSO) on oral candidiasis induced by Candida albicans in immunosu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Victor Onukwube Apeh, Obioma Uzoma Njoku, Fred Okwesili C. Nwodo, Ifeoma Felicia Chukwuma, Adegboyega Abayomi Emmanuel
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Citrullus lanatus Blastospores Candidiasis Fungal burden Renal function Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/31f0bf12ea3c4d77bfb1fc0a98b9589c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:31f0bf12ea3c4d77bfb1fc0a98b9589c
record_format	dspace
spelling	oai:doaj.org-article:31f0bf12ea3c4d77bfb1fc0a98b9589c2021-12-04T04:33:30ZIn silico drug-like properties prediction and in vivo antifungal potentials of Citrullus lanatus seed oil against Candida albicans1878-535210.1016/j.arabjc.2021.103578https://doaj.org/article/31f0bf12ea3c4d77bfb1fc0a98b9589c2022-02-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1878535221005931https://doaj.org/toc/1878-5352Citrullus lanatus seed is an important but neglected seed rich in essential fatty acids. The study sought to investigate in silico and in vivo antifungal activity of some bioactive compounds of Citrullus lanatus (watermelon) seed oil (CLSO) on oral candidiasis induced by Candida albicans in immunosuppressed female albino rats and to predict the Absorption, Distribution, Metabolism and Excretion (ADME) properties of isolated natural compound. Docking studies was performed using standard procedure, standard microbiological and histopathological techniques was employed for study of in vivo antifungal activity of the oil; as well as renal function tests at days 7 and 14 post-infection treatments. Treated groups were compared with that of the control groups. In vitro studies showed varied zone of inhibitions at different concentrations. Ligand-protein interaction showed better binding activity between Palmitic acid and SAP-5 as well as CYP51 when compared with fluconazole (reference drug). Treatment with CLSO showed that there was a significant reduction in the kidney fungal burden (cfu/ml/g) of rats treated with CLSO after 14 days post-infection treatment, compared to group 2 (untreated control) rats. Histomorphology of group 2 showed multifocal aggregation and widespread distribution of fungal blastospores when compared with CLSO-treated groups, which had minimal fungal blastospores in the renal tissues. Thus, in silico and histological data agreed with the findings in microbiology. Furthermore, within the CLSO treated group, a significant increase in the serum concentrations of creatinine was observed, while no significant difference in blood urea values was recorded after day 14 post-infection study. Linoleic and palmitic acid could be considered as a potential antifungal drug candidate with palmitic acid playing a significant role.Victor Onukwube ApehObioma Uzoma NjokuFred Okwesili C. NwodoIfeoma Felicia ChukwumaAdegboyega Abayomi EmmanuelElsevierarticleCitrullus lanatusBlastosporesCandidiasisFungal burdenRenal functionChemistryQD1-999ENArabian Journal of Chemistry, Vol 15, Iss 2, Pp 103578- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Citrullus lanatus Blastospores Candidiasis Fungal burden Renal function Chemistry QD1-999
spellingShingle	Citrullus lanatus Blastospores Candidiasis Fungal burden Renal function Chemistry QD1-999 Victor Onukwube Apeh Obioma Uzoma Njoku Fred Okwesili C. Nwodo Ifeoma Felicia Chukwuma Adegboyega Abayomi Emmanuel In silico drug-like properties prediction and in vivo antifungal potentials of Citrullus lanatus seed oil against Candida albicans
description	Citrullus lanatus seed is an important but neglected seed rich in essential fatty acids. The study sought to investigate in silico and in vivo antifungal activity of some bioactive compounds of Citrullus lanatus (watermelon) seed oil (CLSO) on oral candidiasis induced by Candida albicans in immunosuppressed female albino rats and to predict the Absorption, Distribution, Metabolism and Excretion (ADME) properties of isolated natural compound. Docking studies was performed using standard procedure, standard microbiological and histopathological techniques was employed for study of in vivo antifungal activity of the oil; as well as renal function tests at days 7 and 14 post-infection treatments. Treated groups were compared with that of the control groups. In vitro studies showed varied zone of inhibitions at different concentrations. Ligand-protein interaction showed better binding activity between Palmitic acid and SAP-5 as well as CYP51 when compared with fluconazole (reference drug). Treatment with CLSO showed that there was a significant reduction in the kidney fungal burden (cfu/ml/g) of rats treated with CLSO after 14 days post-infection treatment, compared to group 2 (untreated control) rats. Histomorphology of group 2 showed multifocal aggregation and widespread distribution of fungal blastospores when compared with CLSO-treated groups, which had minimal fungal blastospores in the renal tissues. Thus, in silico and histological data agreed with the findings in microbiology. Furthermore, within the CLSO treated group, a significant increase in the serum concentrations of creatinine was observed, while no significant difference in blood urea values was recorded after day 14 post-infection study. Linoleic and palmitic acid could be considered as a potential antifungal drug candidate with palmitic acid playing a significant role.
format	article
author	Victor Onukwube Apeh Obioma Uzoma Njoku Fred Okwesili C. Nwodo Ifeoma Felicia Chukwuma Adegboyega Abayomi Emmanuel
author_facet	Victor Onukwube Apeh Obioma Uzoma Njoku Fred Okwesili C. Nwodo Ifeoma Felicia Chukwuma Adegboyega Abayomi Emmanuel
author_sort	Victor Onukwube Apeh
title	In silico drug-like properties prediction and in vivo antifungal potentials of Citrullus lanatus seed oil against Candida albicans
title_short	In silico drug-like properties prediction and in vivo antifungal potentials of Citrullus lanatus seed oil against Candida albicans
title_full	In silico drug-like properties prediction and in vivo antifungal potentials of Citrullus lanatus seed oil against Candida albicans
title_fullStr	In silico drug-like properties prediction and in vivo antifungal potentials of Citrullus lanatus seed oil against Candida albicans
title_full_unstemmed	In silico drug-like properties prediction and in vivo antifungal potentials of Citrullus lanatus seed oil against Candida albicans
title_sort	in silico drug-like properties prediction and in vivo antifungal potentials of citrullus lanatus seed oil against candida albicans
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/31f0bf12ea3c4d77bfb1fc0a98b9589c
work_keys_str_mv	AT victoronukwubeapeh insilicodruglikepropertiespredictionandinvivoantifungalpotentialsofcitrulluslanatusseedoilagainstcandidaalbicans AT obiomauzomanjoku insilicodruglikepropertiespredictionandinvivoantifungalpotentialsofcitrulluslanatusseedoilagainstcandidaalbicans AT fredokwesilicnwodo insilicodruglikepropertiespredictionandinvivoantifungalpotentialsofcitrulluslanatusseedoilagainstcandidaalbicans AT ifeomafeliciachukwuma insilicodruglikepropertiespredictionandinvivoantifungalpotentialsofcitrulluslanatusseedoilagainstcandidaalbicans AT adegboyegaabayomiemmanuel insilicodruglikepropertiespredictionandinvivoantifungalpotentialsofcitrulluslanatusseedoilagainstcandidaalbicans
_version_	1718372960324026368

In silico drug-like properties prediction and in vivo antifungal potentials of Citrullus lanatus seed oil against Candida albicans

Ejemplares similares