Mechanism of MicroRNA Regulating the Progress of Atherosclerosis in ApoE-deficient Mice

MicroRNAs play important roles in atherosclerogenesis, and are important novel pharmaceutic targets in atherosclerosis management. The whole spectrum of miRNAs dysregulation is still under intense investigation. This study intends to identify more novel dysregulated microRNAs in atherosclerotic mice...

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Autores principales:	Xiaoqian Lou, Dawei Wang, Zehui Gu, Tengteng Li, Liqun Ren
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	microrna atherosclerosis apoe-deficient mouse gene regulation bioinformatic analysis Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/31f1e6936e6244b2bfde57bea98ed3e2
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spelling	oai:doaj.org-article:31f1e6936e6244b2bfde57bea98ed3e22021-11-17T14:21:59ZMechanism of MicroRNA Regulating the Progress of Atherosclerosis in ApoE-deficient Mice2165-59792165-598710.1080/21655979.2021.2004979https://doaj.org/article/31f1e6936e6244b2bfde57bea98ed3e22021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2004979https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987MicroRNAs play important roles in atherosclerogenesis, and are important novel pharmaceutic targets in atherosclerosis management. The whole spectrum of miRNAs dysregulation is still under intense investigation. This study intends to identify more novel dysregulated microRNAs in atherosclerotic mice. Half of eight-week-old male ApoE-/- mice were fed with high-fat-diet for 12 weeks as a model mice, and remaining half of ApoE-/- mice were fed with a normal-diet as a control. A serum lipid profile was performed with ELISA kits, and atherosclerotic lesions were assessed. Aortic tissues were dissected for gene expression profiling using a Multispecies miRNA 4.0 Array, and significant differentially expressed miRNAs were identified with fold change ≥ 2 and p<0.05. Real-Time quantitative PCR was used to validate microarray gene expression data on selected genes. Predicted target genes were extracted and subjected to bioinformatic analysis for molecular function and pathway enrichment analysis. Model mice showed a 15.32% atherosclerotic lesion compared to 1.52% in the control group. A total of 25 significant differentially expressed microRNAs were identified, with most of them (24/25) downregulated. Real-Time quantitative PCR confirmed the GeneChip data. Bioinformatic analysis of predicted target genes identified high involvement of the PI3K/Akt/mTOR signaling pathway. Microarray profiling of miRNAs in high-fat-fed Model mice identified 25 differentially expressed miRNAs, including some novel miRNAs, and the PI3K/Akt/mTOR signaling pathway is highly enriched in the predicted target genes. The novel identified dysregulated miRNAs suggest a broader spectrum of miRNA dysregulation in the progression of atherosclerosis and provide more research and therapeutic targets for atherosclerosis.Xiaoqian LouDawei WangZehui GuTengteng LiLiqun RenTaylor & Francis Grouparticlemicrornaatherosclerosisapoe-deficient mousegene regulationbioinformatic analysisBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microrna atherosclerosis apoe-deficient mouse gene regulation bioinformatic analysis Biotechnology TP248.13-248.65
spellingShingle	microrna atherosclerosis apoe-deficient mouse gene regulation bioinformatic analysis Biotechnology TP248.13-248.65 Xiaoqian Lou Dawei Wang Zehui Gu Tengteng Li Liqun Ren Mechanism of MicroRNA Regulating the Progress of Atherosclerosis in ApoE-deficient Mice
description	MicroRNAs play important roles in atherosclerogenesis, and are important novel pharmaceutic targets in atherosclerosis management. The whole spectrum of miRNAs dysregulation is still under intense investigation. This study intends to identify more novel dysregulated microRNAs in atherosclerotic mice. Half of eight-week-old male ApoE-/- mice were fed with high-fat-diet for 12 weeks as a model mice, and remaining half of ApoE-/- mice were fed with a normal-diet as a control. A serum lipid profile was performed with ELISA kits, and atherosclerotic lesions were assessed. Aortic tissues were dissected for gene expression profiling using a Multispecies miRNA 4.0 Array, and significant differentially expressed miRNAs were identified with fold change ≥ 2 and p<0.05. Real-Time quantitative PCR was used to validate microarray gene expression data on selected genes. Predicted target genes were extracted and subjected to bioinformatic analysis for molecular function and pathway enrichment analysis. Model mice showed a 15.32% atherosclerotic lesion compared to 1.52% in the control group. A total of 25 significant differentially expressed microRNAs were identified, with most of them (24/25) downregulated. Real-Time quantitative PCR confirmed the GeneChip data. Bioinformatic analysis of predicted target genes identified high involvement of the PI3K/Akt/mTOR signaling pathway. Microarray profiling of miRNAs in high-fat-fed Model mice identified 25 differentially expressed miRNAs, including some novel miRNAs, and the PI3K/Akt/mTOR signaling pathway is highly enriched in the predicted target genes. The novel identified dysregulated miRNAs suggest a broader spectrum of miRNA dysregulation in the progression of atherosclerosis and provide more research and therapeutic targets for atherosclerosis.
format	article
author	Xiaoqian Lou Dawei Wang Zehui Gu Tengteng Li Liqun Ren
author_facet	Xiaoqian Lou Dawei Wang Zehui Gu Tengteng Li Liqun Ren
author_sort	Xiaoqian Lou
title	Mechanism of MicroRNA Regulating the Progress of Atherosclerosis in ApoE-deficient Mice
title_short	Mechanism of MicroRNA Regulating the Progress of Atherosclerosis in ApoE-deficient Mice
title_full	Mechanism of MicroRNA Regulating the Progress of Atherosclerosis in ApoE-deficient Mice
title_fullStr	Mechanism of MicroRNA Regulating the Progress of Atherosclerosis in ApoE-deficient Mice
title_full_unstemmed	Mechanism of MicroRNA Regulating the Progress of Atherosclerosis in ApoE-deficient Mice
title_sort	mechanism of microrna regulating the progress of atherosclerosis in apoe-deficient mice
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/31f1e6936e6244b2bfde57bea98ed3e2
work_keys_str_mv	AT xiaoqianlou mechanismofmicrornaregulatingtheprogressofatherosclerosisinapoedeficientmice AT daweiwang mechanismofmicrornaregulatingtheprogressofatherosclerosisinapoedeficientmice AT zehuigu mechanismofmicrornaregulatingtheprogressofatherosclerosisinapoedeficientmice AT tengtengli mechanismofmicrornaregulatingtheprogressofatherosclerosisinapoedeficientmice AT liqunren mechanismofmicrornaregulatingtheprogressofatherosclerosisinapoedeficientmice
_version_	1718425404022194176

Mechanism of MicroRNA Regulating the Progress of Atherosclerosis in ApoE-deficient Mice

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