Deoxyarbutin displays antitumour activity against melanoma in vitro and in vivo through a p38-mediated mitochondria associated apoptotic pathway

Abstract Deoxyarbutin (DeoxyArbutin, dA), a natural compound widely used in skin lighting, displayed selectively cytotoxicity in vitro. In the study, we found that dA significantly inhibited viability/proliferation of B16F10 melanoma cells, induced tumour cell arrest and apoptosis. Furthermore, dA t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Limei Ma, Yao Xu, Zeliang Wei, Guang Xin, Zhihua Xing, Hai Niu, Wen Huang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/31fb0d4e71574eacab51e39e777470da
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:31fb0d4e71574eacab51e39e777470da
record_format	dspace
spelling	oai:doaj.org-article:31fb0d4e71574eacab51e39e777470da2021-12-02T16:06:30ZDeoxyarbutin displays antitumour activity against melanoma in vitro and in vivo through a p38-mediated mitochondria associated apoptotic pathway10.1038/s41598-017-05416-82045-2322https://doaj.org/article/31fb0d4e71574eacab51e39e777470da2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05416-8https://doaj.org/toc/2045-2322Abstract Deoxyarbutin (DeoxyArbutin, dA), a natural compound widely used in skin lighting, displayed selectively cytotoxicity in vitro. In the study, we found that dA significantly inhibited viability/proliferation of B16F10 melanoma cells, induced tumour cell arrest and apoptosis. Furthermore, dA triggered its pro-apoptosis through damaging the mitochondrial function (membrane potential loss, ATP depletion and ROS overload generation etc.) and activating caspase-9, PARP, caspase-3 and the phosphorylation of p38. Treatment with p38 agonist confirmed the involvement of p38 pathway triggered by dA in B16F10 cells. The in vivo finding also revealed that administration of dA significantly decreased the tumour volume and tumour metastasis in B16F10 xenograft model by inhibiting tumour proliferation and inducing tumour apoptosis. Importantly, the results indicated that dA was specific against tumour cell lines and had no observed systemic toxicity in vivo. Taken together, our study demonstrated that dA could combate tumour in vitro and in vivo by inhibiting the proliferation and metastasis of tumour via a p38-mediated mitochondria associated apoptotic pathway.Limei MaYao XuZeliang WeiGuang XinZhihua XingHai NiuWen HuangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Limei Ma Yao Xu Zeliang Wei Guang Xin Zhihua Xing Hai Niu Wen Huang Deoxyarbutin displays antitumour activity against melanoma in vitro and in vivo through a p38-mediated mitochondria associated apoptotic pathway
description	Abstract Deoxyarbutin (DeoxyArbutin, dA), a natural compound widely used in skin lighting, displayed selectively cytotoxicity in vitro. In the study, we found that dA significantly inhibited viability/proliferation of B16F10 melanoma cells, induced tumour cell arrest and apoptosis. Furthermore, dA triggered its pro-apoptosis through damaging the mitochondrial function (membrane potential loss, ATP depletion and ROS overload generation etc.) and activating caspase-9, PARP, caspase-3 and the phosphorylation of p38. Treatment with p38 agonist confirmed the involvement of p38 pathway triggered by dA in B16F10 cells. The in vivo finding also revealed that administration of dA significantly decreased the tumour volume and tumour metastasis in B16F10 xenograft model by inhibiting tumour proliferation and inducing tumour apoptosis. Importantly, the results indicated that dA was specific against tumour cell lines and had no observed systemic toxicity in vivo. Taken together, our study demonstrated that dA could combate tumour in vitro and in vivo by inhibiting the proliferation and metastasis of tumour via a p38-mediated mitochondria associated apoptotic pathway.
format	article
author	Limei Ma Yao Xu Zeliang Wei Guang Xin Zhihua Xing Hai Niu Wen Huang
author_facet	Limei Ma Yao Xu Zeliang Wei Guang Xin Zhihua Xing Hai Niu Wen Huang
author_sort	Limei Ma
title	Deoxyarbutin displays antitumour activity against melanoma in vitro and in vivo through a p38-mediated mitochondria associated apoptotic pathway
title_short	Deoxyarbutin displays antitumour activity against melanoma in vitro and in vivo through a p38-mediated mitochondria associated apoptotic pathway
title_full	Deoxyarbutin displays antitumour activity against melanoma in vitro and in vivo through a p38-mediated mitochondria associated apoptotic pathway
title_fullStr	Deoxyarbutin displays antitumour activity against melanoma in vitro and in vivo through a p38-mediated mitochondria associated apoptotic pathway
title_full_unstemmed	Deoxyarbutin displays antitumour activity against melanoma in vitro and in vivo through a p38-mediated mitochondria associated apoptotic pathway
title_sort	deoxyarbutin displays antitumour activity against melanoma in vitro and in vivo through a p38-mediated mitochondria associated apoptotic pathway
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/31fb0d4e71574eacab51e39e777470da
work_keys_str_mv	AT limeima deoxyarbutindisplaysantitumouractivityagainstmelanomainvitroandinvivothroughap38mediatedmitochondriaassociatedapoptoticpathway AT yaoxu deoxyarbutindisplaysantitumouractivityagainstmelanomainvitroandinvivothroughap38mediatedmitochondriaassociatedapoptoticpathway AT zeliangwei deoxyarbutindisplaysantitumouractivityagainstmelanomainvitroandinvivothroughap38mediatedmitochondriaassociatedapoptoticpathway AT guangxin deoxyarbutindisplaysantitumouractivityagainstmelanomainvitroandinvivothroughap38mediatedmitochondriaassociatedapoptoticpathway AT zhihuaxing deoxyarbutindisplaysantitumouractivityagainstmelanomainvitroandinvivothroughap38mediatedmitochondriaassociatedapoptoticpathway AT hainiu deoxyarbutindisplaysantitumouractivityagainstmelanomainvitroandinvivothroughap38mediatedmitochondriaassociatedapoptoticpathway AT wenhuang deoxyarbutindisplaysantitumouractivityagainstmelanomainvitroandinvivothroughap38mediatedmitochondriaassociatedapoptoticpathway
_version_	1718384953787416576

Deoxyarbutin displays antitumour activity against melanoma in vitro and in vivo through a p38-mediated mitochondria associated apoptotic pathway

Ejemplares similares