High-throughput 16S rRNA gene sequencing of the microbial community associated with palm oil mill effluents of two oil processing systems

Abstract Palm Oil Mill Effluents (POME) are complex fermentative substrates which habour diverse native microbial contaminants. However, knowledge on the microbiota community shift caused by the anthropogenic effects of POME in the environment is up to date still to be extensively documented. In thi...

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Autores principales:	Benedicte Ella Zranseu Aka, Theodore N’dede Djeni, Simon Laurent Tiemele Amoikon, Jan Kannengiesser, Naaila Ouazzani, Marcellin Koffi Dje
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3205323695ac46949b34fa8ac0081f31
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spelling	oai:doaj.org-article:3205323695ac46949b34fa8ac0081f312021-12-02T18:02:43ZHigh-throughput 16S rRNA gene sequencing of the microbial community associated with palm oil mill effluents of two oil processing systems10.1038/s41598-021-92513-42045-2322https://doaj.org/article/3205323695ac46949b34fa8ac0081f312021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92513-4https://doaj.org/toc/2045-2322Abstract Palm Oil Mill Effluents (POME) are complex fermentative substrates which habour diverse native microbial contaminants. However, knowledge on the microbiota community shift caused by the anthropogenic effects of POME in the environment is up to date still to be extensively documented. In this study, the bacterial and archaeal communities of POME from two palm oil processing systems (artisanal and industrial) were investigated by Illumina MiSeq Platform. Despite the common characteristics of these wastewaters, we found that their microbial communities were significantly different with regard to their diversity and relative abundance of their different Amplicon Sequence Variants (ASV). Indeed, POME from industrial plants harboured as dominant phyla Firmicutes (46.24%), Bacteroidetes (34.19%), Proteobacteria (15.11%), with the particular presence of Spirochaetes, verrucomicrobia and Synergistetes, while those from artisanal production were colonized by Firmicutes (92.06%), Proteobacteria (4.21%) and Actinobacteria (2.09%). Furthermore, 43 AVSs of archaea were detected only in POME from industrial plants and assigned to Crenarchaeota, Diapherotrites, Euryarchaeota and Nanoarchaeaeota phyla, populated mainly by many methane-forming archaea. Definitively, the microbial community composition of POME from both type of processing was markedly different, showing that the history of these ecosystems and various processing conditions have a great impact on each microbial community structure and diversity. By improving knowledge about this microbiome, the results also provide insight into the potential microbial contaminants of soils and rivers receiving these wastewaters.Benedicte Ella Zranseu AkaTheodore N’dede DjeniSimon Laurent Tiemele AmoikonJan KannengiesserNaaila OuazzaniMarcellin Koffi DjeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Benedicte Ella Zranseu Aka Theodore N’dede Djeni Simon Laurent Tiemele Amoikon Jan Kannengiesser Naaila Ouazzani Marcellin Koffi Dje High-throughput 16S rRNA gene sequencing of the microbial community associated with palm oil mill effluents of two oil processing systems
description	Abstract Palm Oil Mill Effluents (POME) are complex fermentative substrates which habour diverse native microbial contaminants. However, knowledge on the microbiota community shift caused by the anthropogenic effects of POME in the environment is up to date still to be extensively documented. In this study, the bacterial and archaeal communities of POME from two palm oil processing systems (artisanal and industrial) were investigated by Illumina MiSeq Platform. Despite the common characteristics of these wastewaters, we found that their microbial communities were significantly different with regard to their diversity and relative abundance of their different Amplicon Sequence Variants (ASV). Indeed, POME from industrial plants harboured as dominant phyla Firmicutes (46.24%), Bacteroidetes (34.19%), Proteobacteria (15.11%), with the particular presence of Spirochaetes, verrucomicrobia and Synergistetes, while those from artisanal production were colonized by Firmicutes (92.06%), Proteobacteria (4.21%) and Actinobacteria (2.09%). Furthermore, 43 AVSs of archaea were detected only in POME from industrial plants and assigned to Crenarchaeota, Diapherotrites, Euryarchaeota and Nanoarchaeaeota phyla, populated mainly by many methane-forming archaea. Definitively, the microbial community composition of POME from both type of processing was markedly different, showing that the history of these ecosystems and various processing conditions have a great impact on each microbial community structure and diversity. By improving knowledge about this microbiome, the results also provide insight into the potential microbial contaminants of soils and rivers receiving these wastewaters.
format	article
author	Benedicte Ella Zranseu Aka Theodore N’dede Djeni Simon Laurent Tiemele Amoikon Jan Kannengiesser Naaila Ouazzani Marcellin Koffi Dje
author_facet	Benedicte Ella Zranseu Aka Theodore N’dede Djeni Simon Laurent Tiemele Amoikon Jan Kannengiesser Naaila Ouazzani Marcellin Koffi Dje
author_sort	Benedicte Ella Zranseu Aka
title	High-throughput 16S rRNA gene sequencing of the microbial community associated with palm oil mill effluents of two oil processing systems
title_short	High-throughput 16S rRNA gene sequencing of the microbial community associated with palm oil mill effluents of two oil processing systems
title_full	High-throughput 16S rRNA gene sequencing of the microbial community associated with palm oil mill effluents of two oil processing systems
title_fullStr	High-throughput 16S rRNA gene sequencing of the microbial community associated with palm oil mill effluents of two oil processing systems
title_full_unstemmed	High-throughput 16S rRNA gene sequencing of the microbial community associated with palm oil mill effluents of two oil processing systems
title_sort	high-throughput 16s rrna gene sequencing of the microbial community associated with palm oil mill effluents of two oil processing systems
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3205323695ac46949b34fa8ac0081f31
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High-throughput 16S rRNA gene sequencing of the microbial community associated with palm oil mill effluents of two oil processing systems

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