Methanolic extract of coconut (Cocos nucifera L.) haustorium mitigates pro-oxidant-mediated apoptotic cell death via Nrf-2 pathway and lipopolysaccharide-induced cytokine release in cells

Cocos nucifera L is well-known for its edible and medicinal uses; multiple products from the different parts of the plant are being used by various communities in India. A less explored product is the coconut haustorium, the solid endosperm portion, which is highly nutritious. The present study firs...

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Autores principales:	Hak-Jae Kim, Arunaksharan Narayanankutty, Anju Sasidharan, Joice Tom Job, Young Ock Kim, Sae Won Na, Rajakrishnan Rajagopal, Ahmed Alfarhan, Bander Al-Munqedhi
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Antioxidant activity Coconut haustorium Cytokine release Cytoprotective effect Anti-inflammatory activity Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/320f02c04bd24d828cf7c1d684bc1dc2
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spelling	oai:doaj.org-article:320f02c04bd24d828cf7c1d684bc1dc22021-11-28T04:28:46ZMethanolic extract of coconut (Cocos nucifera L.) haustorium mitigates pro-oxidant-mediated apoptotic cell death via Nrf-2 pathway and lipopolysaccharide-induced cytokine release in cells1018-364710.1016/j.jksus.2021.101715https://doaj.org/article/320f02c04bd24d828cf7c1d684bc1dc22022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1018364721003773https://doaj.org/toc/1018-3647Cocos nucifera L is well-known for its edible and medicinal uses; multiple products from the different parts of the plant are being used by various communities in India. A less explored product is the coconut haustorium, the solid endosperm portion, which is highly nutritious. The present study first time evaluated the antioxidant, anti-inflammatory and cytoprotective properties of methanolic extract of Coconut haustorium (CHE). The cytoprotective effect of CHE was evaluated in hydrogen peroxide/malondialdehyde-induced toxicity IEC-6 (normal intestinal cell). Initially the biologically safe concentration of CHE and IC50 value of hydrogen peroxide and malondialdehyde was estimated by MTT assay. Treatment with H2O2 and MDA led to glutathione depletion and reduction in the catalase and glutathione reductase activity; which resulted in the increased oxidative stress and thereby cell death. Pretreatment with the different concentrations (10, 20 and 40 µg/mL) of CHE offered significant and dose-dependent protection against the toxicity induced by hydrogen peroxide and malondialdehyde. It is observed that the CHE pretreatment restored the antioxidant enzyme activities and GSH levels back to normal; the improvement in antioxidant status may be through the upregulation of Nrf2/HO1 pathway as observed in CHE pretreated cells. The anti-inflammatory potential was evaluated in the lipopolysaccharide (LPS-1 µg/mL) induced model in macrophages (Raw 264.7 cells). CHE pretreatment reduced the LPS- induced release of cytokines such as IL-1β, IL-6, and tumor necrosis factor-alpha (TNF-α). The results indicated the antioxidant, anti-inflammatory and cytoprotective effect of less explored haustorium from Coconut.Hak-Jae KimArunaksharan NarayanankuttyAnju SasidharanJoice Tom JobYoung Ock KimSae Won NaRajakrishnan RajagopalAhmed AlfarhanBander Al-MunqedhiElsevierarticleAntioxidant activityCoconut haustoriumCytokine releaseCytoprotective effectAnti-inflammatory activityScience (General)Q1-390ENJournal of King Saud University: Science, Vol 34, Iss 1, Pp 101715- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Antioxidant activity Coconut haustorium Cytokine release Cytoprotective effect Anti-inflammatory activity Science (General) Q1-390
spellingShingle	Antioxidant activity Coconut haustorium Cytokine release Cytoprotective effect Anti-inflammatory activity Science (General) Q1-390 Hak-Jae Kim Arunaksharan Narayanankutty Anju Sasidharan Joice Tom Job Young Ock Kim Sae Won Na Rajakrishnan Rajagopal Ahmed Alfarhan Bander Al-Munqedhi Methanolic extract of coconut (Cocos nucifera L.) haustorium mitigates pro-oxidant-mediated apoptotic cell death via Nrf-2 pathway and lipopolysaccharide-induced cytokine release in cells
description	Cocos nucifera L is well-known for its edible and medicinal uses; multiple products from the different parts of the plant are being used by various communities in India. A less explored product is the coconut haustorium, the solid endosperm portion, which is highly nutritious. The present study first time evaluated the antioxidant, anti-inflammatory and cytoprotective properties of methanolic extract of Coconut haustorium (CHE). The cytoprotective effect of CHE was evaluated in hydrogen peroxide/malondialdehyde-induced toxicity IEC-6 (normal intestinal cell). Initially the biologically safe concentration of CHE and IC50 value of hydrogen peroxide and malondialdehyde was estimated by MTT assay. Treatment with H2O2 and MDA led to glutathione depletion and reduction in the catalase and glutathione reductase activity; which resulted in the increased oxidative stress and thereby cell death. Pretreatment with the different concentrations (10, 20 and 40 µg/mL) of CHE offered significant and dose-dependent protection against the toxicity induced by hydrogen peroxide and malondialdehyde. It is observed that the CHE pretreatment restored the antioxidant enzyme activities and GSH levels back to normal; the improvement in antioxidant status may be through the upregulation of Nrf2/HO1 pathway as observed in CHE pretreated cells. The anti-inflammatory potential was evaluated in the lipopolysaccharide (LPS-1 µg/mL) induced model in macrophages (Raw 264.7 cells). CHE pretreatment reduced the LPS- induced release of cytokines such as IL-1β, IL-6, and tumor necrosis factor-alpha (TNF-α). The results indicated the antioxidant, anti-inflammatory and cytoprotective effect of less explored haustorium from Coconut.
format	article
author	Hak-Jae Kim Arunaksharan Narayanankutty Anju Sasidharan Joice Tom Job Young Ock Kim Sae Won Na Rajakrishnan Rajagopal Ahmed Alfarhan Bander Al-Munqedhi
author_facet	Hak-Jae Kim Arunaksharan Narayanankutty Anju Sasidharan Joice Tom Job Young Ock Kim Sae Won Na Rajakrishnan Rajagopal Ahmed Alfarhan Bander Al-Munqedhi
author_sort	Hak-Jae Kim
title	Methanolic extract of coconut (Cocos nucifera L.) haustorium mitigates pro-oxidant-mediated apoptotic cell death via Nrf-2 pathway and lipopolysaccharide-induced cytokine release in cells
title_short	Methanolic extract of coconut (Cocos nucifera L.) haustorium mitigates pro-oxidant-mediated apoptotic cell death via Nrf-2 pathway and lipopolysaccharide-induced cytokine release in cells
title_full	Methanolic extract of coconut (Cocos nucifera L.) haustorium mitigates pro-oxidant-mediated apoptotic cell death via Nrf-2 pathway and lipopolysaccharide-induced cytokine release in cells
title_fullStr	Methanolic extract of coconut (Cocos nucifera L.) haustorium mitigates pro-oxidant-mediated apoptotic cell death via Nrf-2 pathway and lipopolysaccharide-induced cytokine release in cells
title_full_unstemmed	Methanolic extract of coconut (Cocos nucifera L.) haustorium mitigates pro-oxidant-mediated apoptotic cell death via Nrf-2 pathway and lipopolysaccharide-induced cytokine release in cells
title_sort	methanolic extract of coconut (cocos nucifera l.) haustorium mitigates pro-oxidant-mediated apoptotic cell death via nrf-2 pathway and lipopolysaccharide-induced cytokine release in cells
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/320f02c04bd24d828cf7c1d684bc1dc2
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Methanolic extract of coconut (Cocos nucifera L.) haustorium mitigates pro-oxidant-mediated apoptotic cell death via Nrf-2 pathway and lipopolysaccharide-induced cytokine release in cells

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