Prokinetic effects of diatrizoate meglumine (Gastrografin®) in a zebrafish for opioid-induced constipation model

Constipation is a common disease that reduces life quality. Drugs of various mechanisms are being developed to resolve this affliction. Intestinal motility can be easily monitored in zebrafish, and so we selected this organism to develop a constipation model to measure drug-induced prokinetic effect...

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Autores principales:	Dongwoo Kim, Soonil Koun, Seung Young Kim, Young Ran Ha, Jung Wan Choe, Sung Woo Jung, Jong Jin Hyun, Young Kul Jung, Ja Seol Koo, Hyung Joon Yim, Sang Woo Lee
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	zebrafish constipation gastrografin Medicine (General) R5-920 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/32141268c19f4170883a3e75b345b2c6
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spelling	oai:doaj.org-article:32141268c19f4170883a3e75b345b2c62021-11-04T15:00:43ZProkinetic effects of diatrizoate meglumine (Gastrografin®) in a zebrafish for opioid-induced constipation model1976-83542151-248510.1080/19768354.2021.1991472https://doaj.org/article/32141268c19f4170883a3e75b345b2c62021-10-01T00:00:00Zhttp://dx.doi.org/10.1080/19768354.2021.1991472https://doaj.org/toc/1976-8354https://doaj.org/toc/2151-2485Constipation is a common disease that reduces life quality. Drugs of various mechanisms are being developed to resolve this affliction. Intestinal motility can be easily monitored in zebrafish, and so we selected this organism to develop a constipation model to measure drug-induced prokinetic effects. In this study, intestinal motility was monitored in zebrafish by tracking intestinal transit using fluorescence, after which an opioid-induced constipation model was established using loperamide. We then evaluated the prokinetic effect of diatrizoate meglumine (Gastrografin®), which has been empirically used to treat post-operative ileus or adhesive small bowel obstructions. Diatrizoate meglumine was effective in promoting bowel movements in an opioid-induced zebrafish constipation model and its prokinetic effect was associated with an increased expression of interstitial cells of Cajal (ICC) markers. Therefore, the loperamide-induced zebrafish constipation model developed herein is a promising tool to evaluate novel constipation therapies.Dongwoo KimSoonil KounSeung Young KimYoung Ran HaJung Wan ChoeSung Woo JungJong Jin HyunYoung Kul JungJa Seol KooHyung Joon YimSang Woo LeeTaylor & Francis GrouparticlezebrafishconstipationgastrografinMedicine (General)R5-920Biology (General)QH301-705.5ENAnimal Cells and Systems, Vol 0, Iss 0, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	zebrafish constipation gastrografin Medicine (General) R5-920 Biology (General) QH301-705.5
spellingShingle	zebrafish constipation gastrografin Medicine (General) R5-920 Biology (General) QH301-705.5 Dongwoo Kim Soonil Koun Seung Young Kim Young Ran Ha Jung Wan Choe Sung Woo Jung Jong Jin Hyun Young Kul Jung Ja Seol Koo Hyung Joon Yim Sang Woo Lee Prokinetic effects of diatrizoate meglumine (Gastrografin®) in a zebrafish for opioid-induced constipation model
description	Constipation is a common disease that reduces life quality. Drugs of various mechanisms are being developed to resolve this affliction. Intestinal motility can be easily monitored in zebrafish, and so we selected this organism to develop a constipation model to measure drug-induced prokinetic effects. In this study, intestinal motility was monitored in zebrafish by tracking intestinal transit using fluorescence, after which an opioid-induced constipation model was established using loperamide. We then evaluated the prokinetic effect of diatrizoate meglumine (Gastrografin®), which has been empirically used to treat post-operative ileus or adhesive small bowel obstructions. Diatrizoate meglumine was effective in promoting bowel movements in an opioid-induced zebrafish constipation model and its prokinetic effect was associated with an increased expression of interstitial cells of Cajal (ICC) markers. Therefore, the loperamide-induced zebrafish constipation model developed herein is a promising tool to evaluate novel constipation therapies.
format	article
author	Dongwoo Kim Soonil Koun Seung Young Kim Young Ran Ha Jung Wan Choe Sung Woo Jung Jong Jin Hyun Young Kul Jung Ja Seol Koo Hyung Joon Yim Sang Woo Lee
author_facet	Dongwoo Kim Soonil Koun Seung Young Kim Young Ran Ha Jung Wan Choe Sung Woo Jung Jong Jin Hyun Young Kul Jung Ja Seol Koo Hyung Joon Yim Sang Woo Lee
author_sort	Dongwoo Kim
title	Prokinetic effects of diatrizoate meglumine (Gastrografin®) in a zebrafish for opioid-induced constipation model
title_short	Prokinetic effects of diatrizoate meglumine (Gastrografin®) in a zebrafish for opioid-induced constipation model
title_full	Prokinetic effects of diatrizoate meglumine (Gastrografin®) in a zebrafish for opioid-induced constipation model
title_fullStr	Prokinetic effects of diatrizoate meglumine (Gastrografin®) in a zebrafish for opioid-induced constipation model
title_full_unstemmed	Prokinetic effects of diatrizoate meglumine (Gastrografin®) in a zebrafish for opioid-induced constipation model
title_sort	prokinetic effects of diatrizoate meglumine (gastrografin®) in a zebrafish for opioid-induced constipation model
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/32141268c19f4170883a3e75b345b2c6
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Prokinetic effects of diatrizoate meglumine (Gastrografin®) in a zebrafish for opioid-induced constipation model

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