Genome-wide identification and expression analysis of the xyloglucan endotransglucosylase/hydrolase gene family in poplar

Abstract Background Xyloglucan endotransglucosylase/hydrolase (XTH) family plays an important role in cell wall reconstruction and stress resistance in plants. However, the detailed characteristics of XTH family genes and their expression pattern under salt stress have not been reported in poplar. R...

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Autores principales:	Zihan Cheng, Xuemei Zhang, Wenjing Yao, Yuan Gao, Kai Zhao, Qing Guo, Boru Zhou, Tingbo Jiang
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Poplar XTH Expression patterns Salt stress Biotechnology TP248.13-248.65 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/3215bfe50c894ba79e13f481c9e75d60
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spelling	oai:doaj.org-article:3215bfe50c894ba79e13f481c9e75d602021-11-14T12:25:30ZGenome-wide identification and expression analysis of the xyloglucan endotransglucosylase/hydrolase gene family in poplar10.1186/s12864-021-08134-81471-2164https://doaj.org/article/3215bfe50c894ba79e13f481c9e75d602021-11-01T00:00:00Zhttps://doi.org/10.1186/s12864-021-08134-8https://doaj.org/toc/1471-2164Abstract Background Xyloglucan endotransglucosylase/hydrolase (XTH) family plays an important role in cell wall reconstruction and stress resistance in plants. However, the detailed characteristics of XTH family genes and their expression pattern under salt stress have not been reported in poplar. Results In this study, a total of 43 PtrXTH genes were identified from Populus simonii × Populus nigra, and most of them contain two conserved structures (Glyco_hydro_16 and XET_C domain). The promoters of the PtrXTH genes contain mutiple cis-acting elements related to growth and development and stress responses. Collinearity analysis revealed that the XTH genes from poplar has an evolutionary relationship with other six species, including Eucalyptus robusta, Solanum lycopersicum, Glycine max, Arabidopsis, Zea mays and Oryza sativa. Based on RNA-Seq analysis, the PtrXTH genes have different expression patterns in the roots, stems and leaves, and many of them are highly expressed in the roots. In addition, there are11 differentially expressed PtrXTH genes in the roots, 9 in the stems, and 7 in the leaves under salt stress. In addition, the accuracy of RNA-Seq results was verified by RT-qPCR. Conclusion All the results indicated that XTH family genes may play an important role in tissue specificity and salt stress response. This study will lay a theoretical foundation for further study on molecular function of XTH genes in poplar.Zihan ChengXuemei ZhangWenjing YaoYuan GaoKai ZhaoQing GuoBoru ZhouTingbo JiangBMCarticlePoplarXTHExpression patternsSalt stressBiotechnologyTP248.13-248.65GeneticsQH426-470ENBMC Genomics, Vol 22, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Poplar XTH Expression patterns Salt stress Biotechnology TP248.13-248.65 Genetics QH426-470
spellingShingle	Poplar XTH Expression patterns Salt stress Biotechnology TP248.13-248.65 Genetics QH426-470 Zihan Cheng Xuemei Zhang Wenjing Yao Yuan Gao Kai Zhao Qing Guo Boru Zhou Tingbo Jiang Genome-wide identification and expression analysis of the xyloglucan endotransglucosylase/hydrolase gene family in poplar
description	Abstract Background Xyloglucan endotransglucosylase/hydrolase (XTH) family plays an important role in cell wall reconstruction and stress resistance in plants. However, the detailed characteristics of XTH family genes and their expression pattern under salt stress have not been reported in poplar. Results In this study, a total of 43 PtrXTH genes were identified from Populus simonii × Populus nigra, and most of them contain two conserved structures (Glyco_hydro_16 and XET_C domain). The promoters of the PtrXTH genes contain mutiple cis-acting elements related to growth and development and stress responses. Collinearity analysis revealed that the XTH genes from poplar has an evolutionary relationship with other six species, including Eucalyptus robusta, Solanum lycopersicum, Glycine max, Arabidopsis, Zea mays and Oryza sativa. Based on RNA-Seq analysis, the PtrXTH genes have different expression patterns in the roots, stems and leaves, and many of them are highly expressed in the roots. In addition, there are11 differentially expressed PtrXTH genes in the roots, 9 in the stems, and 7 in the leaves under salt stress. In addition, the accuracy of RNA-Seq results was verified by RT-qPCR. Conclusion All the results indicated that XTH family genes may play an important role in tissue specificity and salt stress response. This study will lay a theoretical foundation for further study on molecular function of XTH genes in poplar.
format	article
author	Zihan Cheng Xuemei Zhang Wenjing Yao Yuan Gao Kai Zhao Qing Guo Boru Zhou Tingbo Jiang
author_facet	Zihan Cheng Xuemei Zhang Wenjing Yao Yuan Gao Kai Zhao Qing Guo Boru Zhou Tingbo Jiang
author_sort	Zihan Cheng
title	Genome-wide identification and expression analysis of the xyloglucan endotransglucosylase/hydrolase gene family in poplar
title_short	Genome-wide identification and expression analysis of the xyloglucan endotransglucosylase/hydrolase gene family in poplar
title_full	Genome-wide identification and expression analysis of the xyloglucan endotransglucosylase/hydrolase gene family in poplar
title_fullStr	Genome-wide identification and expression analysis of the xyloglucan endotransglucosylase/hydrolase gene family in poplar
title_full_unstemmed	Genome-wide identification and expression analysis of the xyloglucan endotransglucosylase/hydrolase gene family in poplar
title_sort	genome-wide identification and expression analysis of the xyloglucan endotransglucosylase/hydrolase gene family in poplar
publisher	BMC
publishDate	2021
url	https://doaj.org/article/3215bfe50c894ba79e13f481c9e75d60
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Genome-wide identification and expression analysis of the xyloglucan endotransglucosylase/hydrolase gene family in poplar

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