Deciphering role of technical bioprocess parameters for bioethanol production using microalgae

Microalgae biomass is considered an important feedstock for biofuels and other bioactive compounds due to its faster growth rate, high biomass production and high biomolecules accumulation over first and second-generation feedstock. This research aimed to maximize the specific growth rate of fresh w...

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Autores principales:	Farhana Bibi, Humaira Yasmin, Asif Jamal, Mohammad S. AL-Harbi, Mushtaq Ahmad, Muhammad Zafar, Bashir Ahmad, Bassem N. Samra, Atef F. Ahmed, Muhammad Ishtiaq Ali
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Microalgae Biomass productivity Specific growth rate Optimization RSM Bioethanol Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/321e009568fb453db90c9faad6ed0a8c
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spelling	oai:doaj.org-article:321e009568fb453db90c9faad6ed0a8c2021-11-20T04:57:25ZDeciphering role of technical bioprocess parameters for bioethanol production using microalgae1319-562X10.1016/j.sjbs.2021.10.011https://doaj.org/article/321e009568fb453db90c9faad6ed0a8c2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1319562X21008913https://doaj.org/toc/1319-562XMicroalgae biomass is considered an important feedstock for biofuels and other bioactive compounds due to its faster growth rate, high biomass production and high biomolecules accumulation over first and second-generation feedstock. This research aimed to maximize the specific growth rate of fresh water green microalgae Closteriopsis acicularis, a member of family Chlorellaceae under the effect of pH and phosphate concentration to attain enhanced biomass productivity. This study investigates the individual and cumulative effect of phosphate concentration and pH on specific growth characteristics of Closteriopsis acicularis in autotrophic mode of cultivation for bioethanol production. Central-Composite Design (CCD) strategy and Response Surface Methodology (RSM) was used for the optimization of microalga growth and ethanol production under laboratory conditions. The results showed that high specific growth rate and biomass productivity of 0.342 day−1 and 0.497 g L−1 day−1 respectively, were achieved at high concentration of phosphate (0.115 g L−1) and pH (9) at 21st day of cultivation. The elemental composition of optimized biomass has shown enhanced elemental accumulation of certain macro (C, O, P) and micronutrients (Na, Mg, Al, K, Ca and Fe) except for nitrogen and sulfur. The Fourier transform infrared spectroscopic analysis has revealed spectral peaks and high absorbance in spectral range of carbohydrates, lipids and proteins, in optimized biomass. The carbohydrates content of optimized biomass was observed as 58%, with 29.3 g L−1 of fermentable sugars after acid catalyzed saccharification. The bioethanol yield was estimated as 51 % g ethanol/g glucose with maximum of 14.9 g/L of bioethanol production. In conclusion, it can be inferred that high specific growth rate and biomass productivity can be achieved by varying levels of phosphate concentration and pH during cultivation of Closteriopsis acicularis for improved yield of microbial growth, biomass and bioethanol production.Farhana BibiHumaira YasminAsif JamalMohammad S. AL-HarbiMushtaq AhmadMuhammad ZafarBashir AhmadBassem N. SamraAtef F. AhmedMuhammad Ishtiaq AliElsevierarticleMicroalgaeBiomass productivitySpecific growth rateOptimizationRSMBioethanolBiology (General)QH301-705.5ENSaudi Journal of Biological Sciences, Vol 28, Iss 12, Pp 7595-7606 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microalgae Biomass productivity Specific growth rate Optimization RSM Bioethanol Biology (General) QH301-705.5
spellingShingle	Microalgae Biomass productivity Specific growth rate Optimization RSM Bioethanol Biology (General) QH301-705.5 Farhana Bibi Humaira Yasmin Asif Jamal Mohammad S. AL-Harbi Mushtaq Ahmad Muhammad Zafar Bashir Ahmad Bassem N. Samra Atef F. Ahmed Muhammad Ishtiaq Ali Deciphering role of technical bioprocess parameters for bioethanol production using microalgae
description	Microalgae biomass is considered an important feedstock for biofuels and other bioactive compounds due to its faster growth rate, high biomass production and high biomolecules accumulation over first and second-generation feedstock. This research aimed to maximize the specific growth rate of fresh water green microalgae Closteriopsis acicularis, a member of family Chlorellaceae under the effect of pH and phosphate concentration to attain enhanced biomass productivity. This study investigates the individual and cumulative effect of phosphate concentration and pH on specific growth characteristics of Closteriopsis acicularis in autotrophic mode of cultivation for bioethanol production. Central-Composite Design (CCD) strategy and Response Surface Methodology (RSM) was used for the optimization of microalga growth and ethanol production under laboratory conditions. The results showed that high specific growth rate and biomass productivity of 0.342 day−1 and 0.497 g L−1 day−1 respectively, were achieved at high concentration of phosphate (0.115 g L−1) and pH (9) at 21st day of cultivation. The elemental composition of optimized biomass has shown enhanced elemental accumulation of certain macro (C, O, P) and micronutrients (Na, Mg, Al, K, Ca and Fe) except for nitrogen and sulfur. The Fourier transform infrared spectroscopic analysis has revealed spectral peaks and high absorbance in spectral range of carbohydrates, lipids and proteins, in optimized biomass. The carbohydrates content of optimized biomass was observed as 58%, with 29.3 g L−1 of fermentable sugars after acid catalyzed saccharification. The bioethanol yield was estimated as 51 % g ethanol/g glucose with maximum of 14.9 g/L of bioethanol production. In conclusion, it can be inferred that high specific growth rate and biomass productivity can be achieved by varying levels of phosphate concentration and pH during cultivation of Closteriopsis acicularis for improved yield of microbial growth, biomass and bioethanol production.
format	article
author	Farhana Bibi Humaira Yasmin Asif Jamal Mohammad S. AL-Harbi Mushtaq Ahmad Muhammad Zafar Bashir Ahmad Bassem N. Samra Atef F. Ahmed Muhammad Ishtiaq Ali
author_facet	Farhana Bibi Humaira Yasmin Asif Jamal Mohammad S. AL-Harbi Mushtaq Ahmad Muhammad Zafar Bashir Ahmad Bassem N. Samra Atef F. Ahmed Muhammad Ishtiaq Ali
author_sort	Farhana Bibi
title	Deciphering role of technical bioprocess parameters for bioethanol production using microalgae
title_short	Deciphering role of technical bioprocess parameters for bioethanol production using microalgae
title_full	Deciphering role of technical bioprocess parameters for bioethanol production using microalgae
title_fullStr	Deciphering role of technical bioprocess parameters for bioethanol production using microalgae
title_full_unstemmed	Deciphering role of technical bioprocess parameters for bioethanol production using microalgae
title_sort	deciphering role of technical bioprocess parameters for bioethanol production using microalgae
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/321e009568fb453db90c9faad6ed0a8c
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Deciphering role of technical bioprocess parameters for bioethanol production using microalgae

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