The communication factor EDF and the toxin-antitoxin module mazEF determine the mode of action of antibiotics.

It was recently reported that the production of Reactive Oxygen Species (ROS) is a common mechanism of cell death induced by bactericidal antibiotics. Here we show that triggering the Escherichia coli chromosomal toxin-antitoxin system mazEF is an additional determinant in the mode of action of some...

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Autores principales:	Ilana Kolodkin-Gal, Boaz Sat, Alex Keshet, Hanna Engelberg-Kulka
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/32235f56692046e48fef0f8af5da62f5
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spelling	oai:doaj.org-article:32235f56692046e48fef0f8af5da62f52021-11-25T05:33:53ZThe communication factor EDF and the toxin-antitoxin module mazEF determine the mode of action of antibiotics.1544-91731545-788510.1371/journal.pbio.0060319https://doaj.org/article/32235f56692046e48fef0f8af5da62f52008-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19090622/pdf/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885It was recently reported that the production of Reactive Oxygen Species (ROS) is a common mechanism of cell death induced by bactericidal antibiotics. Here we show that triggering the Escherichia coli chromosomal toxin-antitoxin system mazEF is an additional determinant in the mode of action of some antibiotics. We treated E. coli cultures by antibiotics belonging to one of two groups: (i) Inhibitors of transcription and/or translation, and (ii) DNA damaging. We found that antibiotics of both groups caused: (i) mazEF-mediated cell death, and (ii) the production of ROS through MazF action. However, only antibiotics of the first group caused mazEF-mediated cell death that is ROS-dependent, whereas those of the second group caused mazEF-mediated cell death by an ROS-independent pathway. Furthermore, our results showed that the mode of action of antibiotics was determined by the ability of E. coli cells to communicate through the signaling molecule Extracellular Death Factor (EDF) participating in mazEF induction.Ilana Kolodkin-GalBoaz SatAlex KeshetHanna Engelberg-KulkaPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 6, Iss 12, p e319 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Ilana Kolodkin-Gal Boaz Sat Alex Keshet Hanna Engelberg-Kulka The communication factor EDF and the toxin-antitoxin module mazEF determine the mode of action of antibiotics.
description	It was recently reported that the production of Reactive Oxygen Species (ROS) is a common mechanism of cell death induced by bactericidal antibiotics. Here we show that triggering the Escherichia coli chromosomal toxin-antitoxin system mazEF is an additional determinant in the mode of action of some antibiotics. We treated E. coli cultures by antibiotics belonging to one of two groups: (i) Inhibitors of transcription and/or translation, and (ii) DNA damaging. We found that antibiotics of both groups caused: (i) mazEF-mediated cell death, and (ii) the production of ROS through MazF action. However, only antibiotics of the first group caused mazEF-mediated cell death that is ROS-dependent, whereas those of the second group caused mazEF-mediated cell death by an ROS-independent pathway. Furthermore, our results showed that the mode of action of antibiotics was determined by the ability of E. coli cells to communicate through the signaling molecule Extracellular Death Factor (EDF) participating in mazEF induction.
format	article
author	Ilana Kolodkin-Gal Boaz Sat Alex Keshet Hanna Engelberg-Kulka
author_facet	Ilana Kolodkin-Gal Boaz Sat Alex Keshet Hanna Engelberg-Kulka
author_sort	Ilana Kolodkin-Gal
title	The communication factor EDF and the toxin-antitoxin module mazEF determine the mode of action of antibiotics.
title_short	The communication factor EDF and the toxin-antitoxin module mazEF determine the mode of action of antibiotics.
title_full	The communication factor EDF and the toxin-antitoxin module mazEF determine the mode of action of antibiotics.
title_fullStr	The communication factor EDF and the toxin-antitoxin module mazEF determine the mode of action of antibiotics.
title_full_unstemmed	The communication factor EDF and the toxin-antitoxin module mazEF determine the mode of action of antibiotics.
title_sort	communication factor edf and the toxin-antitoxin module mazef determine the mode of action of antibiotics.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/32235f56692046e48fef0f8af5da62f5
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The communication factor EDF and the toxin-antitoxin module mazEF determine the mode of action of antibiotics.

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