IgG from patients with systemic sclerosis bind to DNA antitopoisomerase 1 in normal human fibroblasts extracts

Mathieu C Tamby1, Am&eacute;lie Servettaz1, Nicolas Tamas1, Joseph Reinbolt2, Fr&eacute;d&eacute;ric Caux3, Olivier Meyer4, Yannick Allanore5, Andr&eacute; Kahan5, Lo&iuml;c Guillevin6, Luc Mouthon1,61Paris-Descartes University, Faculty of Medicine, UPRES-EA 4...

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Autores principales:	Mathieu C Tamby, Am&eacute;lie Servettaz, Nicolas Tamas, Joseph Reinbolt, Fr&eacute;d&eacute;ric Caux, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2008
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/32354f6eefa74440aa561ee762c445e9
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spelling	oai:doaj.org-article:32354f6eefa74440aa561ee762c445e92021-12-02T07:42:51ZIgG from patients with systemic sclerosis bind to DNA antitopoisomerase 1 in normal human fibroblasts extracts1177-54751177-5491https://doaj.org/article/32354f6eefa74440aa561ee762c445e92008-09-01T00:00:00Zhttp://www.dovepress.com/igg-from-patients-with-systemic-sclerosis-bind-to-dna-antitopoisomeras-a2308https://doaj.org/toc/1177-5475https://doaj.org/toc/1177-5491Mathieu C Tamby1, Am&eacute;lie Servettaz1, Nicolas Tamas1, Joseph Reinbolt2, Fr&eacute;d&eacute;ric Caux3, Olivier Meyer4, Yannick Allanore5, Andr&eacute; Kahan5, Lo&iuml;c Guillevin6, Luc Mouthon1,61Paris-Descartes University, Faculty of Medicine, UPRES-EA 4058, Paris, France; 2UPR 9002, Centre National de la Recherche Scientifique, Strasbourg, France; 3UPRES EA 2436, Paris-Nord University, Bobigny, France; 4Rheumatology Department, Bichat Hospital, Assistance Publique-H&ocirc;pitaux de Paris (AP-HP), Paris, France; 5Rheumatology A Department, Cochin Hospital, AP-HP; 6Paris-Descartes University, Faculty of Medicine, Department of Internal Medicine and French Reference Center for Necrotizing Vasculitides and Systemic Sclerosis, Cochin Hospital, AP-HP, Paris, FranceAbstract: By using a semi-quantitative immunoblotting technique, we have analyzed serum immunoglobulin G (IgG) reactivities of patients with limited cutaneous systemic sclerosis and anticentromere antibodies, patients with diffuse systemic sclerosis and antitopoisomerase 1 antibodies, patients with diffuse systemic sclerosis without antitopoisomerase 1 or anticentromere antibodies and age- and gender-matched healthy controls with normal human skin fibroblasts and HEp-2 cells antigens. Serum IgG reactivities of patients with diffuse systemic sclerosis and antitopoisomerase 1 antibodies differed significantly from those of healthy controls or systemic sclerosis patients in other groups for reactivity with fibroblast proteins. IgG from patients with antitopoisomerase 1 antibodies bound to a 90 kDa fibroblast band and to a 100 kDa protein band in a HEp-2 cell protein extract. These two bands were further identified as DNA topoisomerase 1. Our results indicate that IgG from patients with diffuse systemic sclerosis bind DNA topoisomerase 1 in normal human fibroblasts extracts.Keywords: systemic sclerosis, autoantibodies, IgG, fibroblast, DNA topoisomerase 1 Mathieu C TambyAm&eacute;lie ServettazNicolas TamasJoseph ReinboltFr&eacute;d&eacute;ric Cauxet alDove Medical PressarticleMedicine (General)R5-920ENBiologics: Targets & Therapy, Vol 2008, Iss Issue 3, Pp 583-591 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Mathieu C Tamby Am&eacute;lie Servettaz Nicolas Tamas Joseph Reinbolt Fr&eacute;d&eacute;ric Caux et al IgG from patients with systemic sclerosis bind to DNA antitopoisomerase 1 in normal human fibroblasts extracts
description	Mathieu C Tamby1, Am&eacute;lie Servettaz1, Nicolas Tamas1, Joseph Reinbolt2, Fr&eacute;d&eacute;ric Caux3, Olivier Meyer4, Yannick Allanore5, Andr&eacute; Kahan5, Lo&iuml;c Guillevin6, Luc Mouthon1,61Paris-Descartes University, Faculty of Medicine, UPRES-EA 4058, Paris, France; 2UPR 9002, Centre National de la Recherche Scientifique, Strasbourg, France; 3UPRES EA 2436, Paris-Nord University, Bobigny, France; 4Rheumatology Department, Bichat Hospital, Assistance Publique-H&ocirc;pitaux de Paris (AP-HP), Paris, France; 5Rheumatology A Department, Cochin Hospital, AP-HP; 6Paris-Descartes University, Faculty of Medicine, Department of Internal Medicine and French Reference Center for Necrotizing Vasculitides and Systemic Sclerosis, Cochin Hospital, AP-HP, Paris, FranceAbstract: By using a semi-quantitative immunoblotting technique, we have analyzed serum immunoglobulin G (IgG) reactivities of patients with limited cutaneous systemic sclerosis and anticentromere antibodies, patients with diffuse systemic sclerosis and antitopoisomerase 1 antibodies, patients with diffuse systemic sclerosis without antitopoisomerase 1 or anticentromere antibodies and age- and gender-matched healthy controls with normal human skin fibroblasts and HEp-2 cells antigens. Serum IgG reactivities of patients with diffuse systemic sclerosis and antitopoisomerase 1 antibodies differed significantly from those of healthy controls or systemic sclerosis patients in other groups for reactivity with fibroblast proteins. IgG from patients with antitopoisomerase 1 antibodies bound to a 90 kDa fibroblast band and to a 100 kDa protein band in a HEp-2 cell protein extract. These two bands were further identified as DNA topoisomerase 1. Our results indicate that IgG from patients with diffuse systemic sclerosis bind DNA topoisomerase 1 in normal human fibroblasts extracts.Keywords: systemic sclerosis, autoantibodies, IgG, fibroblast, DNA topoisomerase 1
format	article
author	Mathieu C Tamby Am&eacute;lie Servettaz Nicolas Tamas Joseph Reinbolt Fr&eacute;d&eacute;ric Caux et al
author_facet	Mathieu C Tamby Am&eacute;lie Servettaz Nicolas Tamas Joseph Reinbolt Fr&eacute;d&eacute;ric Caux et al
author_sort	Mathieu C Tamby
title	IgG from patients with systemic sclerosis bind to DNA antitopoisomerase 1 in normal human fibroblasts extracts
title_short	IgG from patients with systemic sclerosis bind to DNA antitopoisomerase 1 in normal human fibroblasts extracts
title_full	IgG from patients with systemic sclerosis bind to DNA antitopoisomerase 1 in normal human fibroblasts extracts
title_fullStr	IgG from patients with systemic sclerosis bind to DNA antitopoisomerase 1 in normal human fibroblasts extracts
title_full_unstemmed	IgG from patients with systemic sclerosis bind to DNA antitopoisomerase 1 in normal human fibroblasts extracts
title_sort	igg from patients with systemic sclerosis bind to dna antitopoisomerase 1 in normal human fibroblasts extracts
publisher	Dove Medical Press
publishDate	2008
url	https://doaj.org/article/32354f6eefa74440aa561ee762c445e9
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IgG from patients with systemic sclerosis bind to DNA antitopoisomerase 1 in normal human fibroblasts extracts

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