The Ameliorative Effects of Saikosaponin in Thioacetamide-Induced Liver Injury and Non-Alcoholic Fatty Liver Disease in Mice

Liver disorders are a major health concern. Saikosaponin-d (SSd) is an effective active ingredient extracted from <i>Bupleurum falcatum</i>, a traditional Chinese medicinal plant, with anti-inflammatory and antioxidant properties. However, its hepatoprotective properties and underlying m...

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Autores principales:	Geng-Ruei Chang, Wei-Li Lin, Tzu-Chun Lin, Huei-Jyuan Liao, Yu-Wen Lu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	fatty liver disease inflammation liver injury saikosaponin-d thioacetamide Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/3238c32aba834798a99f7bf39ccb123a
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spelling	oai:doaj.org-article:3238c32aba834798a99f7bf39ccb123a2021-11-11T16:51:23ZThe Ameliorative Effects of Saikosaponin in Thioacetamide-Induced Liver Injury and Non-Alcoholic Fatty Liver Disease in Mice10.3390/ijms2221113831422-00671661-6596https://doaj.org/article/3238c32aba834798a99f7bf39ccb123a2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11383https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Liver disorders are a major health concern. Saikosaponin-d (SSd) is an effective active ingredient extracted from <i>Bupleurum falcatum</i>, a traditional Chinese medicinal plant, with anti-inflammatory and antioxidant properties. However, its hepatoprotective properties and underlying mechanisms are unknown. We investigated the effects and underlying mechanisms of SSd treatment for thioacetamide (TAA)-induced liver injury and high-fat-diet (HFD)-induced non-alcoholic fatty liver disease (NAFLD) in male C57BL/6 mice. The SSd group showed significantly higher food intake, body weight, and hepatic antioxidative enzymes (catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD)) and lower hepatic cyclooxygenase-2 (COX-2), serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and fibroblast growth factor-21 (FGF21) compared with controls, as well as reduced expression of inflammation-related genes (nuclear factor kappa B (<i>NF-κB</i>) and inducible nitric oxide synthase (<i>iNOS</i>)) messenger RNA (mRNA). In NAFLD mice, SSd reduced serum ALT, AST, triglycerides, fatty acid–binding protein 4 (<i>FABP4</i>) and sterol regulatory element–binding protein 1 (<i>SREBP1</i>) mRNA, and endoplasmic reticulum (ER)-stress-related proteins (phosphorylated eukaryotic initiation factor 2α subunit (p-eIF2α), activating transcription factor 4 (ATF4), and C/EBP homologous protein (CHOP). SSd has a hepatoprotective effect in liver injury by suppressing inflammatory responses and acting as an antioxidant.Geng-Ruei ChangWei-Li LinTzu-Chun LinHuei-Jyuan LiaoYu-Wen LuMDPI AGarticlefatty liver diseaseinflammationliver injurysaikosaponin-dthioacetamideBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11383, p 11383 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	fatty liver disease inflammation liver injury saikosaponin-d thioacetamide Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	fatty liver disease inflammation liver injury saikosaponin-d thioacetamide Biology (General) QH301-705.5 Chemistry QD1-999 Geng-Ruei Chang Wei-Li Lin Tzu-Chun Lin Huei-Jyuan Liao Yu-Wen Lu The Ameliorative Effects of Saikosaponin in Thioacetamide-Induced Liver Injury and Non-Alcoholic Fatty Liver Disease in Mice
description	Liver disorders are a major health concern. Saikosaponin-d (SSd) is an effective active ingredient extracted from <i>Bupleurum falcatum</i>, a traditional Chinese medicinal plant, with anti-inflammatory and antioxidant properties. However, its hepatoprotective properties and underlying mechanisms are unknown. We investigated the effects and underlying mechanisms of SSd treatment for thioacetamide (TAA)-induced liver injury and high-fat-diet (HFD)-induced non-alcoholic fatty liver disease (NAFLD) in male C57BL/6 mice. The SSd group showed significantly higher food intake, body weight, and hepatic antioxidative enzymes (catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD)) and lower hepatic cyclooxygenase-2 (COX-2), serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and fibroblast growth factor-21 (FGF21) compared with controls, as well as reduced expression of inflammation-related genes (nuclear factor kappa B (<i>NF-κB</i>) and inducible nitric oxide synthase (<i>iNOS</i>)) messenger RNA (mRNA). In NAFLD mice, SSd reduced serum ALT, AST, triglycerides, fatty acid–binding protein 4 (<i>FABP4</i>) and sterol regulatory element–binding protein 1 (<i>SREBP1</i>) mRNA, and endoplasmic reticulum (ER)-stress-related proteins (phosphorylated eukaryotic initiation factor 2α subunit (p-eIF2α), activating transcription factor 4 (ATF4), and C/EBP homologous protein (CHOP). SSd has a hepatoprotective effect in liver injury by suppressing inflammatory responses and acting as an antioxidant.
format	article
author	Geng-Ruei Chang Wei-Li Lin Tzu-Chun Lin Huei-Jyuan Liao Yu-Wen Lu
author_facet	Geng-Ruei Chang Wei-Li Lin Tzu-Chun Lin Huei-Jyuan Liao Yu-Wen Lu
author_sort	Geng-Ruei Chang
title	The Ameliorative Effects of Saikosaponin in Thioacetamide-Induced Liver Injury and Non-Alcoholic Fatty Liver Disease in Mice
title_short	The Ameliorative Effects of Saikosaponin in Thioacetamide-Induced Liver Injury and Non-Alcoholic Fatty Liver Disease in Mice
title_full	The Ameliorative Effects of Saikosaponin in Thioacetamide-Induced Liver Injury and Non-Alcoholic Fatty Liver Disease in Mice
title_fullStr	The Ameliorative Effects of Saikosaponin in Thioacetamide-Induced Liver Injury and Non-Alcoholic Fatty Liver Disease in Mice
title_full_unstemmed	The Ameliorative Effects of Saikosaponin in Thioacetamide-Induced Liver Injury and Non-Alcoholic Fatty Liver Disease in Mice
title_sort	ameliorative effects of saikosaponin in thioacetamide-induced liver injury and non-alcoholic fatty liver disease in mice
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3238c32aba834798a99f7bf39ccb123a
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The Ameliorative Effects of Saikosaponin in Thioacetamide-Induced Liver Injury and Non-Alcoholic Fatty Liver Disease in Mice

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