Fighting SARS-CoV-2 with green seaweed Ulva sp. extract: extraction protocol predetermines crude ulvan extract anti-SARS-CoV-2 inhibition properties in in vitro Vero-E6 cells assay

Due to the global COVID-19 pandemic, there is a need to screen for novel compounds with antiviral activity against SARS-COV-2. Here we compared chemical composition and the in vitro anti- SARS-COV-2 activity of two different Ulva sp. crude ulvan extracts: one obtained by an HCl-based and another one...

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Autores principales:	Shai Shefer, Arthur Robin, Alexander Chemodanov, Mario Lebendiker, Robert Bostwick, Lynn Rasmussen, Michael Lishner, Michael Gozin, Alexander Golberg
Formato:	article
Lenguaje:	EN
Publicado:	PeerJ Inc. 2021
Materias:	SARS-CoV-2 Antiviral Seaweed Macroalgae Sulfated polysaccharides Ulva sp. Medicine R
Acceso en línea:	https://doaj.org/article/323939427dc7425eaeeb69f89005588d
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spelling	oai:doaj.org-article:323939427dc7425eaeeb69f89005588d2021-11-17T15:05:09ZFighting SARS-CoV-2 with green seaweed Ulva sp. extract: extraction protocol predetermines crude ulvan extract anti-SARS-CoV-2 inhibition properties in in vitro Vero-E6 cells assay10.7717/peerj.123982167-8359https://doaj.org/article/323939427dc7425eaeeb69f89005588d2021-11-01T00:00:00Zhttps://peerj.com/articles/12398.pdfhttps://peerj.com/articles/12398/https://doaj.org/toc/2167-8359Due to the global COVID-19 pandemic, there is a need to screen for novel compounds with antiviral activity against SARS-COV-2. Here we compared chemical composition and the in vitro anti- SARS-COV-2 activity of two different Ulva sp. crude ulvan extracts: one obtained by an HCl-based and another one by ammonium oxalate-based (AOx) extraction protocols. The composition of the crude extracts was analyzed and their antiviral activity was assessed in a cytopathic effect reduction assay using Vero E6 cells. We show that the extraction protocols have a significant impact on the chemical composition, anti- SARS-COV-2 activity, and cytotoxicity of these ulvan extracts. The ulvan extract based on the AOx protocol had a higher average molecular weight, higher charge, and 11.3-fold higher antiviral activity than HCl-based extract. Our results strongly suggest that further bioassay-guided investigation into bioactivity of compounds found in Ulva sp. ulvan extracts could lead to the discovery of novel anti-SARS-CoV-2 antivirals.Shai SheferArthur RobinAlexander ChemodanovMario LebendikerRobert BostwickLynn RasmussenMichael LishnerMichael GozinAlexander GolbergPeerJ Inc.articleSARS-CoV-2AntiviralSeaweedMacroalgaeSulfated polysaccharidesUlva sp.MedicineRENPeerJ, Vol 9, p e12398 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SARS-CoV-2 Antiviral Seaweed Macroalgae Sulfated polysaccharides Ulva sp. Medicine R
spellingShingle	SARS-CoV-2 Antiviral Seaweed Macroalgae Sulfated polysaccharides Ulva sp. Medicine R Shai Shefer Arthur Robin Alexander Chemodanov Mario Lebendiker Robert Bostwick Lynn Rasmussen Michael Lishner Michael Gozin Alexander Golberg Fighting SARS-CoV-2 with green seaweed Ulva sp. extract: extraction protocol predetermines crude ulvan extract anti-SARS-CoV-2 inhibition properties in in vitro Vero-E6 cells assay
description	Due to the global COVID-19 pandemic, there is a need to screen for novel compounds with antiviral activity against SARS-COV-2. Here we compared chemical composition and the in vitro anti- SARS-COV-2 activity of two different Ulva sp. crude ulvan extracts: one obtained by an HCl-based and another one by ammonium oxalate-based (AOx) extraction protocols. The composition of the crude extracts was analyzed and their antiviral activity was assessed in a cytopathic effect reduction assay using Vero E6 cells. We show that the extraction protocols have a significant impact on the chemical composition, anti- SARS-COV-2 activity, and cytotoxicity of these ulvan extracts. The ulvan extract based on the AOx protocol had a higher average molecular weight, higher charge, and 11.3-fold higher antiviral activity than HCl-based extract. Our results strongly suggest that further bioassay-guided investigation into bioactivity of compounds found in Ulva sp. ulvan extracts could lead to the discovery of novel anti-SARS-CoV-2 antivirals.
format	article
author	Shai Shefer Arthur Robin Alexander Chemodanov Mario Lebendiker Robert Bostwick Lynn Rasmussen Michael Lishner Michael Gozin Alexander Golberg
author_facet	Shai Shefer Arthur Robin Alexander Chemodanov Mario Lebendiker Robert Bostwick Lynn Rasmussen Michael Lishner Michael Gozin Alexander Golberg
author_sort	Shai Shefer
title	Fighting SARS-CoV-2 with green seaweed Ulva sp. extract: extraction protocol predetermines crude ulvan extract anti-SARS-CoV-2 inhibition properties in in vitro Vero-E6 cells assay
title_short	Fighting SARS-CoV-2 with green seaweed Ulva sp. extract: extraction protocol predetermines crude ulvan extract anti-SARS-CoV-2 inhibition properties in in vitro Vero-E6 cells assay
title_full	Fighting SARS-CoV-2 with green seaweed Ulva sp. extract: extraction protocol predetermines crude ulvan extract anti-SARS-CoV-2 inhibition properties in in vitro Vero-E6 cells assay
title_fullStr	Fighting SARS-CoV-2 with green seaweed Ulva sp. extract: extraction protocol predetermines crude ulvan extract anti-SARS-CoV-2 inhibition properties in in vitro Vero-E6 cells assay
title_full_unstemmed	Fighting SARS-CoV-2 with green seaweed Ulva sp. extract: extraction protocol predetermines crude ulvan extract anti-SARS-CoV-2 inhibition properties in in vitro Vero-E6 cells assay
title_sort	fighting sars-cov-2 with green seaweed ulva sp. extract: extraction protocol predetermines crude ulvan extract anti-sars-cov-2 inhibition properties in in vitro vero-e6 cells assay
publisher	PeerJ Inc.
publishDate	2021
url	https://doaj.org/article/323939427dc7425eaeeb69f89005588d
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Fighting SARS-CoV-2 with green seaweed Ulva sp. extract: extraction protocol predetermines crude ulvan extract anti-SARS-CoV-2 inhibition properties in in vitro Vero-E6 cells assay

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