Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever

Abstract Mayaro virus (MAYV), which causes mayaro fever, is endemic to limited regions of South America that may expand due to the possible involvement of Aedes spp. mosquitoes in its transmission. Its effective control will require the accurate identification of infected individuals, which has been...

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Autores principales: Paloma Napoleão-Pêgo, Flávia R. G. Carneiro, Andressa M. Durans, Larissa R. Gomes, Carlos M. Morel, David W. Provance, Salvatore G. De-Simone
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Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/323ba8e1629c4591bc974113fea05550
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spelling oai:doaj.org-article:323ba8e1629c4591bc974113fea055502021-12-02T16:31:48ZPerformance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever10.1038/s41598-021-94817-x2045-2322https://doaj.org/article/323ba8e1629c4591bc974113fea055502021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94817-xhttps://doaj.org/toc/2045-2322Abstract Mayaro virus (MAYV), which causes mayaro fever, is endemic to limited regions of South America that may expand due to the possible involvement of Aedes spp. mosquitoes in its transmission. Its effective control will require the accurate identification of infected individuals, which has been restricted to nucleic acid-based tests due to similarities with other emerging members of the Alphavirus genus of the Togaviridae family; both in structure and clinical symptoms. Serological tests have a more significant potential to expand testing at a reasonable cost, and their performance primarily reflects that of the antigen utilized to capture pathogen-specific antibodies. Here, we describe the assembly of a synthetic gene encoding multiple copies of antigenic determinants mapped from the nsP1, nsP2, E1, and E2 proteins of MAYV that readily expressed as a stable chimeric protein in bacteria. Its serological performance as the target in ELISAs revealed a high accuracy for detecting anti-MAYV IgM antibodies. No cross-reactivity was observed with serum from seropositive individuals for dengue, chikungunya, yellow fever, Zika, and other infectious diseases as well as healthy individuals. Our data suggest that this bioengineered antigen could be used to develop high-performance serological tests for MAYV infections.Paloma Napoleão-PêgoFlávia R. G. CarneiroAndressa M. DuransLarissa R. GomesCarlos M. MorelDavid W. ProvanceSalvatore G. De-SimoneNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Paloma Napoleão-Pêgo
Flávia R. G. Carneiro
Andressa M. Durans
Larissa R. Gomes
Carlos M. Morel
David W. Provance
Salvatore G. De-Simone
Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever
description Abstract Mayaro virus (MAYV), which causes mayaro fever, is endemic to limited regions of South America that may expand due to the possible involvement of Aedes spp. mosquitoes in its transmission. Its effective control will require the accurate identification of infected individuals, which has been restricted to nucleic acid-based tests due to similarities with other emerging members of the Alphavirus genus of the Togaviridae family; both in structure and clinical symptoms. Serological tests have a more significant potential to expand testing at a reasonable cost, and their performance primarily reflects that of the antigen utilized to capture pathogen-specific antibodies. Here, we describe the assembly of a synthetic gene encoding multiple copies of antigenic determinants mapped from the nsP1, nsP2, E1, and E2 proteins of MAYV that readily expressed as a stable chimeric protein in bacteria. Its serological performance as the target in ELISAs revealed a high accuracy for detecting anti-MAYV IgM antibodies. No cross-reactivity was observed with serum from seropositive individuals for dengue, chikungunya, yellow fever, Zika, and other infectious diseases as well as healthy individuals. Our data suggest that this bioengineered antigen could be used to develop high-performance serological tests for MAYV infections.
format article
author Paloma Napoleão-Pêgo
Flávia R. G. Carneiro
Andressa M. Durans
Larissa R. Gomes
Carlos M. Morel
David W. Provance
Salvatore G. De-Simone
author_facet Paloma Napoleão-Pêgo
Flávia R. G. Carneiro
Andressa M. Durans
Larissa R. Gomes
Carlos M. Morel
David W. Provance
Salvatore G. De-Simone
author_sort Paloma Napoleão-Pêgo
title Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever
title_short Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever
title_full Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever
title_fullStr Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever
title_full_unstemmed Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever
title_sort performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute mayaro fever
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/323ba8e1629c4591bc974113fea05550
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