Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever
Abstract Mayaro virus (MAYV), which causes mayaro fever, is endemic to limited regions of South America that may expand due to the possible involvement of Aedes spp. mosquitoes in its transmission. Its effective control will require the accurate identification of infected individuals, which has been...
Guardado en:
Autores principales: | , , , , , , |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2021
|
Materias: | |
Acceso en línea: | https://doaj.org/article/323ba8e1629c4591bc974113fea05550 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:doaj.org-article:323ba8e1629c4591bc974113fea05550 |
---|---|
record_format |
dspace |
spelling |
oai:doaj.org-article:323ba8e1629c4591bc974113fea055502021-12-02T16:31:48ZPerformance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever10.1038/s41598-021-94817-x2045-2322https://doaj.org/article/323ba8e1629c4591bc974113fea055502021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94817-xhttps://doaj.org/toc/2045-2322Abstract Mayaro virus (MAYV), which causes mayaro fever, is endemic to limited regions of South America that may expand due to the possible involvement of Aedes spp. mosquitoes in its transmission. Its effective control will require the accurate identification of infected individuals, which has been restricted to nucleic acid-based tests due to similarities with other emerging members of the Alphavirus genus of the Togaviridae family; both in structure and clinical symptoms. Serological tests have a more significant potential to expand testing at a reasonable cost, and their performance primarily reflects that of the antigen utilized to capture pathogen-specific antibodies. Here, we describe the assembly of a synthetic gene encoding multiple copies of antigenic determinants mapped from the nsP1, nsP2, E1, and E2 proteins of MAYV that readily expressed as a stable chimeric protein in bacteria. Its serological performance as the target in ELISAs revealed a high accuracy for detecting anti-MAYV IgM antibodies. No cross-reactivity was observed with serum from seropositive individuals for dengue, chikungunya, yellow fever, Zika, and other infectious diseases as well as healthy individuals. Our data suggest that this bioengineered antigen could be used to develop high-performance serological tests for MAYV infections.Paloma Napoleão-PêgoFlávia R. G. CarneiroAndressa M. DuransLarissa R. GomesCarlos M. MorelDavid W. ProvanceSalvatore G. De-SimoneNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Paloma Napoleão-Pêgo Flávia R. G. Carneiro Andressa M. Durans Larissa R. Gomes Carlos M. Morel David W. Provance Salvatore G. De-Simone Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever |
description |
Abstract Mayaro virus (MAYV), which causes mayaro fever, is endemic to limited regions of South America that may expand due to the possible involvement of Aedes spp. mosquitoes in its transmission. Its effective control will require the accurate identification of infected individuals, which has been restricted to nucleic acid-based tests due to similarities with other emerging members of the Alphavirus genus of the Togaviridae family; both in structure and clinical symptoms. Serological tests have a more significant potential to expand testing at a reasonable cost, and their performance primarily reflects that of the antigen utilized to capture pathogen-specific antibodies. Here, we describe the assembly of a synthetic gene encoding multiple copies of antigenic determinants mapped from the nsP1, nsP2, E1, and E2 proteins of MAYV that readily expressed as a stable chimeric protein in bacteria. Its serological performance as the target in ELISAs revealed a high accuracy for detecting anti-MAYV IgM antibodies. No cross-reactivity was observed with serum from seropositive individuals for dengue, chikungunya, yellow fever, Zika, and other infectious diseases as well as healthy individuals. Our data suggest that this bioengineered antigen could be used to develop high-performance serological tests for MAYV infections. |
format |
article |
author |
Paloma Napoleão-Pêgo Flávia R. G. Carneiro Andressa M. Durans Larissa R. Gomes Carlos M. Morel David W. Provance Salvatore G. De-Simone |
author_facet |
Paloma Napoleão-Pêgo Flávia R. G. Carneiro Andressa M. Durans Larissa R. Gomes Carlos M. Morel David W. Provance Salvatore G. De-Simone |
author_sort |
Paloma Napoleão-Pêgo |
title |
Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever |
title_short |
Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever |
title_full |
Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever |
title_fullStr |
Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever |
title_full_unstemmed |
Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever |
title_sort |
performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute mayaro fever |
publisher |
Nature Portfolio |
publishDate |
2021 |
url |
https://doaj.org/article/323ba8e1629c4591bc974113fea05550 |
work_keys_str_mv |
AT palomanapoleaopego performanceassessmentofamultiepitopechimericantigenfortheserologicaldiagnosisofacutemayarofever AT flaviargcarneiro performanceassessmentofamultiepitopechimericantigenfortheserologicaldiagnosisofacutemayarofever AT andressamdurans performanceassessmentofamultiepitopechimericantigenfortheserologicaldiagnosisofacutemayarofever AT larissargomes performanceassessmentofamultiepitopechimericantigenfortheserologicaldiagnosisofacutemayarofever AT carlosmmorel performanceassessmentofamultiepitopechimericantigenfortheserologicaldiagnosisofacutemayarofever AT davidwprovance performanceassessmentofamultiepitopechimericantigenfortheserologicaldiagnosisofacutemayarofever AT salvatoregdesimone performanceassessmentofamultiepitopechimericantigenfortheserologicaldiagnosisofacutemayarofever |
_version_ |
1718383861739552768 |