NAC-loaded electrospun scaffolding system with dual compartments for the osteogenesis of rBMSCs in vitro

Yuanjing Zhu,1,* Fangfang Song,1,* Yanyun Ju,2 Liyuan Huang,1 Lu Zhang,1 Chuliang Tang,1 Hongye Yang,1 Cui Huang1 1Center for Smart Materials and Devices, State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, School of Materials Science and Engineering, Wuhan Universit...

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Autores principales:	Zhu Y, Song F, Ju Y, Huang L, Zhang L, Tang C, Yang HY, Huang C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	bone tissue engineering hierarchical scaffold NAC osteogenesis electrospun mesoporous silica nanoparticles Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3243102275ae42fa9692bccb8a6fad98
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spelling	oai:doaj.org-article:3243102275ae42fa9692bccb8a6fad982021-12-02T06:32:10ZNAC-loaded electrospun scaffolding system with dual compartments for the osteogenesis of rBMSCs in vitro1178-2013https://doaj.org/article/3243102275ae42fa9692bccb8a6fad982019-01-01T00:00:00Zhttps://www.dovepress.com/nac-loaded-electrospun-scaffolding-system-with-dual-compartments-for-t-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yuanjing Zhu,1,* Fangfang Song,1,* Yanyun Ju,2 Liyuan Huang,1 Lu Zhang,1 Chuliang Tang,1 Hongye Yang,1 Cui Huang1 1Center for Smart Materials and Devices, State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, School of Materials Science and Engineering, Wuhan University of Technology, Wuhan, Hubei, China; 2Center for Smart Materials and Devices, State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, Hubei, China *These authors contributed equally to this work Purpose: In this study, we aimed to develop a unique N-acetyl cysteine (NAC)-loaded polylactic-co-glycolic acid (PLGA) electrospun system with separate compartments for the promotion of osteogenesis.Materials and methods: We first prepared solutions of NAC-loaded mesoporous silica nanoparticles (MSNs), PLGA, and NAC in N, N-dimethylformamide and tetrahydrofuran for the construction of the electrospun system. We then fed solutions to a specific injector for electrospinning. The physical and chemical properties of the scaffold were characterized through scanning electron microscopy, transmission electron microscopy, and Fourier transform infrared spectroscopy. The release of NAC and Si from different PLGA scaffolds was estimated. The cell viability, cell growth, and osteogenic potential of rat bone marrow-derived stroma cell (rBMSCs) on different PLGA scaffolds were evaluated through MTT assay, live/dead staining, phalloidin staining, and Alizarin red staining. The expression levels of osteogenic-related markers were analyzed through real-time PCR (qRT-PCR).Results: NAC was successfully loaded into MSNs. The addition of MSNs and NAC decreased the diameters of the electrospun fibers, increased the hydrophilicity and mechanical property of the PLGA scaffold. The release kinetic curve indicated that NAC was released from (PLGA + NAC)/(NAC@MSN) in a biphasic pattern, that featured an initial burst release stage and a later sustained release stage. This release pattern of NAC encapsulated on the (PLGA + NAC)/(NAC@MSN) scaffolds enabled to prolong the high concentrations of release of NAC, thus drastically affecting the osteogenic differentiation of rBMSCs.Conclusion: A PLGA electrospun scaffold was developed, and MSNs were used as separate nanocarriers for recharging NAC concentration, demonstrating the promising use of (PLGA + NAC)/(NAC@MSN) for bone tissue engineering. Keywords: bone tissue engineering, N-acetyl cysteine, osteogenesis, electrospun, mesoporous silica nanoparticles, drug compartmentZhu YSong FJu YHuang LZhang LTang CYang HYHuang CDove Medical Pressarticlebone tissue engineeringhierarchical scaffoldNACosteogenesiselectrospunmesoporous silica nanoparticlesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 787-798 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	bone tissue engineering hierarchical scaffold NAC osteogenesis electrospun mesoporous silica nanoparticles Medicine (General) R5-920
spellingShingle	bone tissue engineering hierarchical scaffold NAC osteogenesis electrospun mesoporous silica nanoparticles Medicine (General) R5-920 Zhu Y Song F Ju Y Huang L Zhang L Tang C Yang HY Huang C NAC-loaded electrospun scaffolding system with dual compartments for the osteogenesis of rBMSCs in vitro
description	Yuanjing Zhu,1,* Fangfang Song,1,* Yanyun Ju,2 Liyuan Huang,1 Lu Zhang,1 Chuliang Tang,1 Hongye Yang,1 Cui Huang1 1Center for Smart Materials and Devices, State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, School of Materials Science and Engineering, Wuhan University of Technology, Wuhan, Hubei, China; 2Center for Smart Materials and Devices, State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, Hubei, China *These authors contributed equally to this work Purpose: In this study, we aimed to develop a unique N-acetyl cysteine (NAC)-loaded polylactic-co-glycolic acid (PLGA) electrospun system with separate compartments for the promotion of osteogenesis.Materials and methods: We first prepared solutions of NAC-loaded mesoporous silica nanoparticles (MSNs), PLGA, and NAC in N, N-dimethylformamide and tetrahydrofuran for the construction of the electrospun system. We then fed solutions to a specific injector for electrospinning. The physical and chemical properties of the scaffold were characterized through scanning electron microscopy, transmission electron microscopy, and Fourier transform infrared spectroscopy. The release of NAC and Si from different PLGA scaffolds was estimated. The cell viability, cell growth, and osteogenic potential of rat bone marrow-derived stroma cell (rBMSCs) on different PLGA scaffolds were evaluated through MTT assay, live/dead staining, phalloidin staining, and Alizarin red staining. The expression levels of osteogenic-related markers were analyzed through real-time PCR (qRT-PCR).Results: NAC was successfully loaded into MSNs. The addition of MSNs and NAC decreased the diameters of the electrospun fibers, increased the hydrophilicity and mechanical property of the PLGA scaffold. The release kinetic curve indicated that NAC was released from (PLGA + NAC)/(NAC@MSN) in a biphasic pattern, that featured an initial burst release stage and a later sustained release stage. This release pattern of NAC encapsulated on the (PLGA + NAC)/(NAC@MSN) scaffolds enabled to prolong the high concentrations of release of NAC, thus drastically affecting the osteogenic differentiation of rBMSCs.Conclusion: A PLGA electrospun scaffold was developed, and MSNs were used as separate nanocarriers for recharging NAC concentration, demonstrating the promising use of (PLGA + NAC)/(NAC@MSN) for bone tissue engineering. Keywords: bone tissue engineering, N-acetyl cysteine, osteogenesis, electrospun, mesoporous silica nanoparticles, drug compartment
format	article
author	Zhu Y Song F Ju Y Huang L Zhang L Tang C Yang HY Huang C
author_facet	Zhu Y Song F Ju Y Huang L Zhang L Tang C Yang HY Huang C
author_sort	Zhu Y
title	NAC-loaded electrospun scaffolding system with dual compartments for the osteogenesis of rBMSCs in vitro
title_short	NAC-loaded electrospun scaffolding system with dual compartments for the osteogenesis of rBMSCs in vitro
title_full	NAC-loaded electrospun scaffolding system with dual compartments for the osteogenesis of rBMSCs in vitro
title_fullStr	NAC-loaded electrospun scaffolding system with dual compartments for the osteogenesis of rBMSCs in vitro
title_full_unstemmed	NAC-loaded electrospun scaffolding system with dual compartments for the osteogenesis of rBMSCs in vitro
title_sort	nac-loaded electrospun scaffolding system with dual compartments for the osteogenesis of rbmscs in vitro
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/3243102275ae42fa9692bccb8a6fad98
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NAC-loaded electrospun scaffolding system with dual compartments for the osteogenesis of rBMSCs in vitro

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