Assessment of oxidative damage to proteins and DNA in urine of newborn infants by a validated UPLC-MS/MS approach.

The assessment of oxidative stress is highly relevant in clinical Perinatology as it is associated to adverse outcomes in newborn infants. This study summarizes results from the validation of an Ultra Performance Liquid Chromatography-tandem Mass Spectrometry (UPLC-MS/MS) method for the simultaneous...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Julia Kuligowski, Isabel Torres-Cuevas, Guillermo Quintás, Denise Rook, Johannes B van Goudoever, Elena Cubells, Miguel Asensi, Isabel Lliso, Antonio Nuñez, Máximo Vento, Javier Escobar
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3248e1d73df7451caaad624d4d6f007f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3248e1d73df7451caaad624d4d6f007f
record_format	dspace
spelling	oai:doaj.org-article:3248e1d73df7451caaad624d4d6f007f2021-11-18T08:25:18ZAssessment of oxidative damage to proteins and DNA in urine of newborn infants by a validated UPLC-MS/MS approach.1932-620310.1371/journal.pone.0093703https://doaj.org/article/3248e1d73df7451caaad624d4d6f007f2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24695409/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The assessment of oxidative stress is highly relevant in clinical Perinatology as it is associated to adverse outcomes in newborn infants. This study summarizes results from the validation of an Ultra Performance Liquid Chromatography-tandem Mass Spectrometry (UPLC-MS/MS) method for the simultaneous quantification of the urinary concentrations of a set of endogenous biomarkers, capable to provide a valid snapshot of the oxidative stress status applicable in human clinical trials, especially in the field of Perinatology. The set of analytes included are phenylalanine (Phe), para-tyrosine (p-Tyr), ortho-tyrosine (o-Tyr), meta-tyrosine (m-Tyr), 3-NO2-tyrosine (3NO2-Tyr), 3-Cl-tyrosine (3Cl-Tyr), 2'-deoxyguanosine (2dG) and 8-hydroxy-2'-deoxyguanosine (8OHdG). Following the FDA-based guidelines, appropriate levels of accuracy and precision, as well as adequate levels of sensitivity with limits of detection (LODs) in the low nanomolar (nmol/L) range were confirmed after method validation. The validity of the proposed UPLC-MS/MS method was assessed by analysing urine samples from a clinical trial in extremely low birth weight (ELBW) infants randomized to be resuscitated with two different initial inspiratory fractions of oxygen.Julia KuligowskiIsabel Torres-CuevasGuillermo QuintásDenise RookJohannes B van GoudoeverElena CubellsMiguel AsensiIsabel LlisoAntonio NuñezMáximo VentoJavier EscobarPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 4, p e93703 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Julia Kuligowski Isabel Torres-Cuevas Guillermo Quintás Denise Rook Johannes B van Goudoever Elena Cubells Miguel Asensi Isabel Lliso Antonio Nuñez Máximo Vento Javier Escobar Assessment of oxidative damage to proteins and DNA in urine of newborn infants by a validated UPLC-MS/MS approach.
description	The assessment of oxidative stress is highly relevant in clinical Perinatology as it is associated to adverse outcomes in newborn infants. This study summarizes results from the validation of an Ultra Performance Liquid Chromatography-tandem Mass Spectrometry (UPLC-MS/MS) method for the simultaneous quantification of the urinary concentrations of a set of endogenous biomarkers, capable to provide a valid snapshot of the oxidative stress status applicable in human clinical trials, especially in the field of Perinatology. The set of analytes included are phenylalanine (Phe), para-tyrosine (p-Tyr), ortho-tyrosine (o-Tyr), meta-tyrosine (m-Tyr), 3-NO2-tyrosine (3NO2-Tyr), 3-Cl-tyrosine (3Cl-Tyr), 2'-deoxyguanosine (2dG) and 8-hydroxy-2'-deoxyguanosine (8OHdG). Following the FDA-based guidelines, appropriate levels of accuracy and precision, as well as adequate levels of sensitivity with limits of detection (LODs) in the low nanomolar (nmol/L) range were confirmed after method validation. The validity of the proposed UPLC-MS/MS method was assessed by analysing urine samples from a clinical trial in extremely low birth weight (ELBW) infants randomized to be resuscitated with two different initial inspiratory fractions of oxygen.
format	article
author	Julia Kuligowski Isabel Torres-Cuevas Guillermo Quintás Denise Rook Johannes B van Goudoever Elena Cubells Miguel Asensi Isabel Lliso Antonio Nuñez Máximo Vento Javier Escobar
author_facet	Julia Kuligowski Isabel Torres-Cuevas Guillermo Quintás Denise Rook Johannes B van Goudoever Elena Cubells Miguel Asensi Isabel Lliso Antonio Nuñez Máximo Vento Javier Escobar
author_sort	Julia Kuligowski
title	Assessment of oxidative damage to proteins and DNA in urine of newborn infants by a validated UPLC-MS/MS approach.
title_short	Assessment of oxidative damage to proteins and DNA in urine of newborn infants by a validated UPLC-MS/MS approach.
title_full	Assessment of oxidative damage to proteins and DNA in urine of newborn infants by a validated UPLC-MS/MS approach.
title_fullStr	Assessment of oxidative damage to proteins and DNA in urine of newborn infants by a validated UPLC-MS/MS approach.
title_full_unstemmed	Assessment of oxidative damage to proteins and DNA in urine of newborn infants by a validated UPLC-MS/MS approach.
title_sort	assessment of oxidative damage to proteins and dna in urine of newborn infants by a validated uplc-ms/ms approach.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/3248e1d73df7451caaad624d4d6f007f
work_keys_str_mv	AT juliakuligowski assessmentofoxidativedamagetoproteinsanddnainurineofnewborninfantsbyavalidateduplcmsmsapproach AT isabeltorrescuevas assessmentofoxidativedamagetoproteinsanddnainurineofnewborninfantsbyavalidateduplcmsmsapproach AT guillermoquintas assessmentofoxidativedamagetoproteinsanddnainurineofnewborninfantsbyavalidateduplcmsmsapproach AT deniserook assessmentofoxidativedamagetoproteinsanddnainurineofnewborninfantsbyavalidateduplcmsmsapproach AT johannesbvangoudoever assessmentofoxidativedamagetoproteinsanddnainurineofnewborninfantsbyavalidateduplcmsmsapproach AT elenacubells assessmentofoxidativedamagetoproteinsanddnainurineofnewborninfantsbyavalidateduplcmsmsapproach AT miguelasensi assessmentofoxidativedamagetoproteinsanddnainurineofnewborninfantsbyavalidateduplcmsmsapproach AT isabellliso assessmentofoxidativedamagetoproteinsanddnainurineofnewborninfantsbyavalidateduplcmsmsapproach AT antonionunez assessmentofoxidativedamagetoproteinsanddnainurineofnewborninfantsbyavalidateduplcmsmsapproach AT maximovento assessmentofoxidativedamagetoproteinsanddnainurineofnewborninfantsbyavalidateduplcmsmsapproach AT javierescobar assessmentofoxidativedamagetoproteinsanddnainurineofnewborninfantsbyavalidateduplcmsmsapproach
_version_	1718421794126299136

Assessment of oxidative damage to proteins and DNA in urine of newborn infants by a validated UPLC-MS/MS approach.

Ejemplares similares