Genomic diversity contributes to the neuroinvasiveness of the Yellow fever French neurotropic vaccine

Abstract Mass vaccination with the live attenuated vaccine YF-17D is the current way to prevent infection with Yellow fever virus (YFV). However, 0.000012–0.00002% of vaccinated patients develop post-vaccination neurological syndrome (YEL-AND). Understanding the factors responsible for neuroinvasion...

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Autores principales:	Florian Bakoa, Christophe Préhaud, Guillaume Beauclair, Maxime Chazal, Nathalie Mantel, Monique Lafon, Nolwenn Jouvenet
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/3250ab5afc1042ee8bd3d7363697f181
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spelling	oai:doaj.org-article:3250ab5afc1042ee8bd3d7363697f1812021-12-02T17:14:58ZGenomic diversity contributes to the neuroinvasiveness of the Yellow fever French neurotropic vaccine10.1038/s41541-021-00318-32059-0105https://doaj.org/article/3250ab5afc1042ee8bd3d7363697f1812021-04-01T00:00:00Zhttps://doi.org/10.1038/s41541-021-00318-3https://doaj.org/toc/2059-0105Abstract Mass vaccination with the live attenuated vaccine YF-17D is the current way to prevent infection with Yellow fever virus (YFV). However, 0.000012–0.00002% of vaccinated patients develop post-vaccination neurological syndrome (YEL-AND). Understanding the factors responsible for neuroinvasion, neurotropism, and neurovirulence of the vaccine is critical for improving its biosafety. The YF-FNV vaccine strain, known to be associated with a higher frequency of YEL-AND (0.3–0.4%) than YF-17D, is an excellent model to study vaccine neuroinvasiveness. We determined that neuroinvasiveness of YF-FNV occured both via infection and passage through human brain endothelial cells. Plaque purification and next generation sequencing (NGS) identified several neuroinvasive variants. Their neuroinvasiveness was not higher than that of YF-FNV. However, rebuilding the YF-FNV population diversity from a set of isolated YF-FNV-N variants restored the original neuroinvasive phenotype of YF-FNV. Therefore, we conclude that viral population diversity is a critical factor for YFV vaccine neuroinvasiveness.Florian BakoaChristophe PréhaudGuillaume BeauclairMaxime ChazalNathalie MantelMonique LafonNolwenn JouvenetNature PortfolioarticleImmunologic diseases. AllergyRC581-607Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENnpj Vaccines, Vol 6, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Florian Bakoa Christophe Préhaud Guillaume Beauclair Maxime Chazal Nathalie Mantel Monique Lafon Nolwenn Jouvenet Genomic diversity contributes to the neuroinvasiveness of the Yellow fever French neurotropic vaccine
description	Abstract Mass vaccination with the live attenuated vaccine YF-17D is the current way to prevent infection with Yellow fever virus (YFV). However, 0.000012–0.00002% of vaccinated patients develop post-vaccination neurological syndrome (YEL-AND). Understanding the factors responsible for neuroinvasion, neurotropism, and neurovirulence of the vaccine is critical for improving its biosafety. The YF-FNV vaccine strain, known to be associated with a higher frequency of YEL-AND (0.3–0.4%) than YF-17D, is an excellent model to study vaccine neuroinvasiveness. We determined that neuroinvasiveness of YF-FNV occured both via infection and passage through human brain endothelial cells. Plaque purification and next generation sequencing (NGS) identified several neuroinvasive variants. Their neuroinvasiveness was not higher than that of YF-FNV. However, rebuilding the YF-FNV population diversity from a set of isolated YF-FNV-N variants restored the original neuroinvasive phenotype of YF-FNV. Therefore, we conclude that viral population diversity is a critical factor for YFV vaccine neuroinvasiveness.
format	article
author	Florian Bakoa Christophe Préhaud Guillaume Beauclair Maxime Chazal Nathalie Mantel Monique Lafon Nolwenn Jouvenet
author_facet	Florian Bakoa Christophe Préhaud Guillaume Beauclair Maxime Chazal Nathalie Mantel Monique Lafon Nolwenn Jouvenet
author_sort	Florian Bakoa
title	Genomic diversity contributes to the neuroinvasiveness of the Yellow fever French neurotropic vaccine
title_short	Genomic diversity contributes to the neuroinvasiveness of the Yellow fever French neurotropic vaccine
title_full	Genomic diversity contributes to the neuroinvasiveness of the Yellow fever French neurotropic vaccine
title_fullStr	Genomic diversity contributes to the neuroinvasiveness of the Yellow fever French neurotropic vaccine
title_full_unstemmed	Genomic diversity contributes to the neuroinvasiveness of the Yellow fever French neurotropic vaccine
title_sort	genomic diversity contributes to the neuroinvasiveness of the yellow fever french neurotropic vaccine
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3250ab5afc1042ee8bd3d7363697f181
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Genomic diversity contributes to the neuroinvasiveness of the Yellow fever French neurotropic vaccine

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