Complexes containing cationic and anionic pH-sensitive liposomes: comparative study of factors influencing plasmid DNA gene delivery to tumors

Yan Chen,* Ji Sun,* Ying Lu, Chun Tao, Jingbin Huang, He Zhang, Yuan Yu, Hao Zou, Jing Gao, Yanqiang Zhong Department of Pharmaceutical Science, School of Pharmacy, The Second Military Medical University, Shanghai, People’s Republic of China *These authors contributed equally to this work...

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Autores principales:	Chen Y, Sun J, Lu Y, Tao C, Huang JB, Zhang H, Yu Y, Zou H, Gao J, Zhong YQ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/325324956ada4f1bb94ff6f4bc686b2e
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spelling	oai:doaj.org-article:325324956ada4f1bb94ff6f4bc686b2e2021-12-02T02:36:41ZComplexes containing cationic and anionic pH-sensitive liposomes: comparative study of factors influencing plasmid DNA gene delivery to tumors1176-91141178-2013https://doaj.org/article/325324956ada4f1bb94ff6f4bc686b2e2013-04-01T00:00:00Zhttp://www.dovepress.com/complexes-containing-cationic-and-anionic-ph-sensitive-liposomes-compa-a12850https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Yan Chen,* Ji Sun,* Ying Lu, Chun Tao, Jingbin Huang, He Zhang, Yuan Yu, Hao Zou, Jing Gao, Yanqiang Zhong Department of Pharmaceutical Science, School of Pharmacy, The Second Military Medical University, Shanghai, People’s Republic of China *These authors contributed equally to this work Abstract: pH-sensitive liposomes represent an effective gene vector in cancer therapy. However, their use is greatly hampered by their relatively low transfection efficiency. To improve the transfection efficiency of pH-sensitive liposomes, we prepared complexes containing 3β-[N-(N',N'-dimethylaminoethane) carbamoyl] cholesterol (DC-Chol) and dioleoylphosphatidyl ethanolamine (DOPE) liposomes and pH-sensitive liposomes composed of cholesteryl hemisuccinate (CHEMS) and DOPE, and evaluated the influence of various factors on plasmid DNA (pDNA) transfection efficiency. All DC-Chol/DOPE liposome/pDNA and pH-sensitive liposome complexes showed similarly potent pH sensitivity. In the presence of serum-containing medium, two optimized complexes of DC-Chol/DOPE liposomes/pDNA and pH-sensitive PEGylated liposomes showed high transfection efficiency of 22.94% and 20.07%, respectively. Notably, DC-Chol/DOPE (2:3) liposomes/pH-sensitive PEGylated (1%) liposome complexes with a charge ratio of 1:1 (m/m [+/-]) showed enhanced accumulation in tumors in vivo. Our results show the influence of various factors on pDNA transfection efficiency in complexes of DC-Chol/DOPE liposomes and pH-sensitive PEGylated liposomes. Understanding of such mechanisms will lead to better design of complexes of DC-Chol/DOPE liposomes and pH-sensitive liposomes for gene therapy. Keywords: cationic liposomes, pH-sensitive liposomes, pDNA, transfection, PEGylatedChen YSun JLu YTao CHuang JBZhang HYu YZou HGao JZhong YQDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 1573-1593 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Chen Y Sun J Lu Y Tao C Huang JB Zhang H Yu Y Zou H Gao J Zhong YQ Complexes containing cationic and anionic pH-sensitive liposomes: comparative study of factors influencing plasmid DNA gene delivery to tumors
description	Yan Chen,* Ji Sun,* Ying Lu, Chun Tao, Jingbin Huang, He Zhang, Yuan Yu, Hao Zou, Jing Gao, Yanqiang Zhong Department of Pharmaceutical Science, School of Pharmacy, The Second Military Medical University, Shanghai, People’s Republic of China *These authors contributed equally to this work Abstract: pH-sensitive liposomes represent an effective gene vector in cancer therapy. However, their use is greatly hampered by their relatively low transfection efficiency. To improve the transfection efficiency of pH-sensitive liposomes, we prepared complexes containing 3β-[N-(N',N'-dimethylaminoethane) carbamoyl] cholesterol (DC-Chol) and dioleoylphosphatidyl ethanolamine (DOPE) liposomes and pH-sensitive liposomes composed of cholesteryl hemisuccinate (CHEMS) and DOPE, and evaluated the influence of various factors on plasmid DNA (pDNA) transfection efficiency. All DC-Chol/DOPE liposome/pDNA and pH-sensitive liposome complexes showed similarly potent pH sensitivity. In the presence of serum-containing medium, two optimized complexes of DC-Chol/DOPE liposomes/pDNA and pH-sensitive PEGylated liposomes showed high transfection efficiency of 22.94% and 20.07%, respectively. Notably, DC-Chol/DOPE (2:3) liposomes/pH-sensitive PEGylated (1%) liposome complexes with a charge ratio of 1:1 (m/m [+/-]) showed enhanced accumulation in tumors in vivo. Our results show the influence of various factors on pDNA transfection efficiency in complexes of DC-Chol/DOPE liposomes and pH-sensitive PEGylated liposomes. Understanding of such mechanisms will lead to better design of complexes of DC-Chol/DOPE liposomes and pH-sensitive liposomes for gene therapy. Keywords: cationic liposomes, pH-sensitive liposomes, pDNA, transfection, PEGylated
format	article
author	Chen Y Sun J Lu Y Tao C Huang JB Zhang H Yu Y Zou H Gao J Zhong YQ
author_facet	Chen Y Sun J Lu Y Tao C Huang JB Zhang H Yu Y Zou H Gao J Zhong YQ
author_sort	Chen Y
title	Complexes containing cationic and anionic pH-sensitive liposomes: comparative study of factors influencing plasmid DNA gene delivery to tumors
title_short	Complexes containing cationic and anionic pH-sensitive liposomes: comparative study of factors influencing plasmid DNA gene delivery to tumors
title_full	Complexes containing cationic and anionic pH-sensitive liposomes: comparative study of factors influencing plasmid DNA gene delivery to tumors
title_fullStr	Complexes containing cationic and anionic pH-sensitive liposomes: comparative study of factors influencing plasmid DNA gene delivery to tumors
title_full_unstemmed	Complexes containing cationic and anionic pH-sensitive liposomes: comparative study of factors influencing plasmid DNA gene delivery to tumors
title_sort	complexes containing cationic and anionic ph-sensitive liposomes: comparative study of factors influencing plasmid dna gene delivery to tumors
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/325324956ada4f1bb94ff6f4bc686b2e
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Complexes containing cationic and anionic pH-sensitive liposomes: comparative study of factors influencing plasmid DNA gene delivery to tumors

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